CaptureSelect™ Biotin Anti-IgA Conjugate

CaptureSelect Biotin Anti-IgA Conjugate consists of a 14 kDa recombinant single domain antibody fragment (V_HH affinity ligand) that specifically binds to the α-chain of all human IgA antibodies (Fc domain). The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

The CaptureSelect Biotin Anti-IgA Conjugate allows you to:

• Detect, quantitate, and characterize all human IgA antibodies, including monomeric IgA-1 and IgA-2, dimeric IgA (secretory and non-secretory), and recombinant IgA monoclonal antibodies, as well as total IgA in human plasma and/or serum samples
• Avoid cross-binding with other isotypes, such as IgG, IgM, IgD, and IgE, free light chains, or IgA from bovine sources such as FCS

Applications for CaptureSelect Biotin Anti-IgA Conjugate include ELISA, Western blot, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Animal origin statement

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (V_HH) created by a proprietary technology. The V_HH affinity ligand is a 12–15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).