Nitrocellulose Membranes, 0.2 μm

Name: Nitrocellulose Membranes, 0.2 μm
SKU: 77012

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Número de catálogo	Dimensions (LxW)	Suficiente para
77012	8 x 12 cm	25 transferencias de minigel
88013	7,9 cm x 10,5 cm	15 transferencias de minigel
88024	8 x 8 cm	15 transferencias de minigel

3 opciones

Número de catálogo 77012

Precio (CLP)

Dimensions (LxW):

8 x 12 cm

Suficiente para:

25 transferencias de minigel

Pedido a granel o personalizado

Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods). The 0.2-μm pore size is well-suited for western blotting, and this smaller pore size is ideal for the transfer of low molecular weight proteins or peptides and nucleic acids (<300 bp).

Features include:
100% pure nitrocellulose membrane
• Convenient—save time with pre-cut sheets
• Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• High-binding affinity—provides excellent protein binding, blocks easily, and does not require pre-wetting with alcohol
• Low background—produce high-quality data with low background signal in both chemiluminescent and fluorescent western blotting

For Research Use Only. Not for use in diagnostic procedures.

Especificaciones

DescripciónMembrana de nitrocelulosa Pierce, 0,2 um, 8cm x 12cm

Cantidad25 Pre-cut Membranes

Condiciones de envíoTemperatura ambiente

Dimensions (LxW)8 x 12 cm

FormatoHoja

Longitud (métrico)12 cm

MaterialNitrocelulosa

Tamaño de poro0,2 μm

Suficiente para25 transferencias de minigel

Anchura (métrico)8 cm

Unit SizeEach

Contenido y almacenamiento

Almacene las membranas a temperatura ambiente, lejos de vapores químicos. Algunos vapores de disolventes pueden disolver de manera parcial las membranas, lo que interrumpirá la estructura de los poros. Mantenga las membranas alejadas de la luz solar directa. La luz solar puede hacer que la nitrocelulosa se seque y se vuelva quebradiza. Nota: Los filtros de membrana de nitrocelulosa son muy inflamables. Mantenga el producto alejado del calor, las chispas y el fuego. El punto de inflamación es de aproximadamente 2 °C. Las membranas pueden esterilizarse mediante autoclave a vapor a 121 °C.

Preguntas frecuentes

How can I store, strip, and reuse my western blot?

For nitrocellulose or PVDF membrane following Western blot detection using a chemiluminescent or fluorescent substrate system: Following transfer, air dry the membrane and place in an envelope, preferably on top of a supported surface to keep the membrane flat. The blot can be stored indefinitely at -80 degrees C. When ready to reprobe, prewet the PVDF blot with alcohol for a few seconds, followed by a few rinses with pure water to reduce the alcohol concentration. Then proceed as normal with blocking step.

FOR STRIPPING/REPROBING OF MEMBRANES: Harsh protocol (see NOTE below for modifications)

1) Submerge the membrane in stripping buffer (100 mM BME, 2% SDS, 62.5 mM Tris-HCl, pH 6.7) and incubate at 50 degrees C for 30 min with occasional agitation. If more stringent conditions necessary, incubate at 70 degrees C.

2) Wash 2 x 10 min in TBS-T/PBS-T at room temperature.

3) Block the membrane by immersing in 5% blocking reagent TBS-T or PBS-T for 1 hr at room temperature.

4) Immunodetection

NOTE: Often you don't need such harsh conditions to remove antibodies from their proteins. The stringency of one or several of the variables can be decreased: lower the temperature, decrease the time, less BME, less SDS, etc. An especially mild but still often effective stripping protocol is lower pH incubation. Example: pH 2.0 Tris 50-100 mM, 30-60 min incubation (you may do two incubations if you wish). Then rinse and block as usual. If you do not wish to re-use the membrane immediately after stripping, you can store the membrane in plastic wrap (wet, you do not want it to dry out). Another simple, mild stripping buffer is 0.1 M glycine•HCl (pH 2.5-3.0), incubation 30 min to 2 hrs room temperature or 37 degrees C, depending on the antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are your PVDF and nitrocellulose membranes compatible with the Li-COR instrument?

Yes, both our PVDF and nitrocellulose membranes are compatible with the Li-COR instrument.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.