Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™
Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™
Citas y referencias (5)
Thermo Scientific™

Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™

El cóctel inhibidor de proteasas y fosfatasas Thermo Scientific Halt sin EDTA (100X) proporciona la comodidad de una solución únicaMás información
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Número de catálogoCantidad
784411 ml
7844324 × 100 μl
784455 x 1 ml
7844710 ml
Número de catálogo 78441
Precio (CLP)
300.000
Each
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Cantidad:
1 ml
Pedido a granel o personalizado
Precio (CLP)
300.000
Each
Añadir al carro de la compra
El cóctel inhibidor de proteasas y fosfatasas Thermo Scientific Halt sin EDTA (100X) proporciona la comodidad de una solución única con la protección completa de las muestras de proteínas para lisados de células y tejidos.

Características del cóctel de inhibidores de proteasas y fosfatasas Halt sin EDTA (100X):

• Protección completa: cóctel todo en uno que contiene inhibidores de proteasas y fosfatasas.
• Envases de varios tamaños: seleccione uno de los cuatro prácticos tamaños de envase que se ajustan a prácticamente todas las necesidades.
• Práctico: la formulación del líquido 100X es mucho más sencilla y eficaz que los inhibidores individuales y otros formatos.
• Fácil almacenamiento: el formato con base acuosa permite un práctico almacenamiento en sala fría sin congelación, a diferencia de los cócteles basados en DMSO
• Compatible: se puede usar en espectrometría de masas (MS) porque no contiene AEBSF, lo cual puede provocar que los picos se desplacen.
• Validado: totalmente compatible con los tampones de lisis celular Thermo Scientific Pierce.

El cóctel de inhibidores de proteasas y fosfatasas Halt está específicamente optimizado para proteger las proteínas de la degradación durante la extracción y purificación. El cóctel contiene inhibidores contra las principales clases de proteasas y fosfatasas, por lo que se ocupa de aminopeptidasas, proteasas de cisteína y serina, y fosfatasas de serina/treonina y proteína tirosina.

Productos relacionados
Cóctel de inhibidores de proteasa y fosfatasa Halt™ (100X)

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
TampónTampón de lisis
DescripciónCóctel inhibidor de proteasa y fosfatasa Halt, sin EDTA
InhibidoresInhibidor de la proteasa, inhibidor de la fosfatasa
Cantidad1 ml
Tipo de reactivoInhibidor de la proteasa, inhibidor de la fosfatasa
FormularioLíquido
Línea de productosHalt
Tipo de productoCóctel inhibidor
Unit SizeEach
Contenido y almacenamiento
Tras su recepción a 4 °C. Producto enviado con un paquete de hielo. No congele el producto.

Preguntas frecuentes

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™ FAQs

Citations & References (5)

Citations & References
Abstract
Olaratumab Exerts Antitumor Activity in Preclinical Models of Pediatric Bone and Soft Tissue Tumors through Inhibition of Platelet-Derived Growth Factor Receptor α.
Authors:Lowery CD,Blosser W,Dowless M,Knoche S,Stephens J,Li H,Surguladze D,Loizos N,Luffer-Atlas D,Oakley GJ 3rd,Guo Q,Iyer S,Rubin BP,Stancato L
Journal:Clinical cancer research : an official journal of the American Association for Cancer Research
PubMed ID:29191969
Purpose: Platelet-derived growth factor receptor α (PDGFRα) is implicated in several adult and pediatric malignancies, where activated signaling in tumor cells and/or cells within the microenvironment drive tumorigenesis and disease progression. Olaratumab (LY3012207/IMC-3G3) is a human mAb that exclusively binds to PDGFRα and recently received accelerated FDA approval and conditional ... More
Reverse phase protein microarray technology in traumatic brain injury.
Authors:Gyorgy AB,Walker J,Wingo D,Eidelman O,Pollard HB,Molnar A,Agoston DV
Journal:Journal of neuroscience methods
PubMed ID:20674607
Antibody based, high throughput proteomics technology represents an exciting new approach in understanding the pathobiologies of complex disorders such as cancer, stroke and traumatic brain injury. Reverse phase protein microarray (RPPA) can complement the classical methods based on mass spectrometry as a high throughput validation and quantification method. RPPA technology ... More
Distinct roles for Notch1 and Notch3 in human adipose-derived stem/stromal cell adipogenesis.
Authors:Liu MC,Logan H,Newman JJ
Journal:Molecular biology reports
PubMed ID:33021719
The role of the Notch signaling pathway in adipogenesis has long been controversial as the action of individual Notch receptors appears to vary with experimental conditions. In this study, we offer some explanation for the observed contradictions by comparing the role of both Notch1 and Notch3 in regulating the expression ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
The Src inhibitor dasatinib accelerates the differentiation of human bone marrow-derived mesenchymal stromal cells into osteoblasts.
Authors:Id Boufker H, Lagneaux L, Najar M, Piccart M, Ghanem G, Body JJ, Journé F
Journal:BMC Cancer
PubMed ID:20565769
The proto-oncogene Src is an important non-receptor protein tyrosine kinase involved in signaling pathways that control cell adhesion, growth, migration and differentiation. It negatively regulates osteoblast activity, and, as such, its inhibition is a potential means to prevent bone loss. Dasatinib is a new dual Src/Bcr-Abl tyrosine kinase inhibitor initially ... More