Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™
Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™
Citas y referencias (6)
Thermo Scientific™

Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™

El cóctel inhibidor de proteasas y fosfatasas Thermo Scientific Halt sin EDTA (100X) proporciona la comodidad de una solución únicaMás información
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Número de catálogoCantidad
784455 x 1 ml
784411 ml
7844324 × 100 μl
7844710 ml
Número de catálogo 78445
Precio (CLP)
1.005.708
Each
Añadir al carro de la compra
Cantidad:
5 x 1 ml
Pedido a granel o personalizado
Precio (CLP)
1.005.708
Each
Añadir al carro de la compra
El cóctel inhibidor de proteasas y fosfatasas Thermo Scientific Halt sin EDTA (100X) proporciona la comodidad de una solución única con la protección completa de las muestras de proteínas para lisados de células y tejidos.

Características del cóctel de inhibidores de proteasas y fosfatasas Halt sin EDTA (100X):

• Protección completa: cóctel todo en uno que contiene inhibidores de proteasas y fosfatasas.
• Envases de varios tamaños: seleccione uno de los cuatro prácticos tamaños de envase que se ajustan a prácticamente todas las necesidades.
• Práctico: la formulación del líquido 100X es mucho más sencilla y eficaz que los inhibidores individuales y otros formatos.
• Fácil almacenamiento: el formato con base acuosa permite un práctico almacenamiento en sala fría sin congelación, a diferencia de los cócteles basados en DMSO
• Compatible: se puede usar en espectrometría de masas (MS) porque no contiene AEBSF, lo cual puede provocar que los picos se desplacen.
• Validado: totalmente compatible con los tampones de lisis celular Thermo Scientific Pierce.

El cóctel de inhibidores de proteasas y fosfatasas Halt está específicamente optimizado para proteger las proteínas de la degradación durante la extracción y purificación. El cóctel contiene inhibidores contra las principales clases de proteasas y fosfatasas, por lo que se ocupa de aminopeptidasas, proteasas de cisteína y serina, y fosfatasas de serina/treonina y proteína tirosina.

Productos relacionados
Cóctel de inhibidores de proteasa y fosfatasa Halt™ (100X)

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
TampónTampón de lisis
DescripciónCóctel inhibidor de proteasa y fosfatasa Halt, sin EDTA
InhibidoresInhibidor de la proteasa, inhibidor de la fosfatasa
Cantidad5 x 1 ml
Tipo de reactivoInhibidor de la proteasa, inhibidor de la fosfatasa
FormularioLíquido
Línea de productosHalt
Tipo de productoCóctel inhibidor
Unit SizeEach
Contenido y almacenamiento
Tras su recepción a 4 °C. Producto enviado con un paquete de hielo. No congele el producto.

Preguntas frecuentes

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™ FAQs

Citations & References (6)

Citations & References
Abstract
Impact of carbamazepine on SMARCA4 (BRG1) expression in colorectal cancer: modulation by KRAS mutation status.
Authors:Shaykevich A,Chae D,Silverman I,Bassali J,Louloueian N,Siegman A,Bandyopadhyaya G,Goel S,Maitra R
Journal:Investigational new drugs
PubMed ID:38446332
SMARCA4 is a gene traditionally considered a tumor suppressor. Recent research has however found that SMARCA4 likely promotes cancer growth and is a good target for cancer treatment. The drug carbamazepine, an autophagy inducer, was used on colorectal cancer cell lines, HCT1116 and Hke3 (KRAS mutant and wildtype). Our study ... More
(3'R)-hydroxytabernaelegantine C: A bisindole alkaloid with potent apoptosis inducing activity in colon (HCT116, SW620) and liver (HepG2) cancer cells.
Authors:Paterna A,Gomes SE,Borralho PM,Mulhovo S,Rodrigues CM,Ferreira MU
Journal:Journal of ethnopharmacology
PubMed ID:27616029
ETHNOPHARMACOLOGICAL RELEVANCE: Tabernaemontana elegans Stapf. (Apocynaceae) is a medicinal plant traditionally used in African countries to treat cancer. AIMS OF THE STUDY: To discover new apoptosis inducing lead compounds from T. elegans and provide scientific validation of the ethnopharmacological use of this plant. MATERIALS AND METHODS: Through fractionation, (3'R)-hydroxytaberanelegantine C ... More
Authors:Mansoor TA, Borralho PM, Luo X, Mulhovo S, Rodrigues CM, Ferreira MJ.
Journal:J Nat Prod
PubMed ID:25066282
6-Acetonyldihydrochelerythrine (1), a benzophenanthridine alkaloid, isolated from the methanol extract of Zanthoxylum capense, displayed potent cytotoxic activity in human HCT116 and SW620 colon carcinoma cells, to a higher extent than 5-fluorouracil (5-FU), the cornerstone chemotherapeutic agent in colon cancer. Cytotoxicity of 1 was evaluated by MTS, lactate dehydrogenase (LDH), and ... More
CDK4/6 activity is required during G(2) arrest to prevent stress-induced endoreplication.
Authors:McKenney C,Lendner Y,Guerrero Zuniga A,Sinha N,Veresko B,Aikin TJ,Regot S
Journal:Science (New York, N.Y.)
PubMed ID:38696576
Cell cycle events are coordinated by cyclin-dependent kinases (CDKs) to ensure robust cell division. CDK4/6 and CDK2 regulate the growth 1 (G(1)) to synthesis (S) phase transition of the cell cycle by responding to mitogen signaling, promoting E2F transcription and inhibition of the anaphase-promoting complex. We found that this mechanism ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
View all Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™ citations