Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™
Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™
Citas y referencias (6)
Thermo Scientific™

Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™

El cóctel inhibidor de proteasas y fosfatasas Thermo Scientific Halt sin EDTA (100X) proporciona la comodidad de una solución únicaMás información
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Número de catálogoCantidad
7844710 ml
784411 ml
7844324 × 100 μl
784455 x 1 ml
Número de catálogo 78447
Precio (CLP)
-
Cantidad:
10 ml
Pedido a granel o personalizado
El cóctel inhibidor de proteasas y fosfatasas Thermo Scientific Halt sin EDTA (100X) proporciona la comodidad de una solución única con la protección completa de las muestras de proteínas para lisados de células y tejidos.

Características del cóctel de inhibidores de proteasas y fosfatasas Halt sin EDTA (100X):

• Protección completa: cóctel todo en uno que contiene inhibidores de proteasas y fosfatasas.
• Envases de varios tamaños: seleccione uno de los cuatro prácticos tamaños de envase que se ajustan a prácticamente todas las necesidades.
• Práctico: la formulación del líquido 100X es mucho más sencilla y eficaz que los inhibidores individuales y otros formatos.
• Fácil almacenamiento: el formato con base acuosa permite un práctico almacenamiento en sala fría sin congelación, a diferencia de los cócteles basados en DMSO
• Compatible: se puede usar en espectrometría de masas (MS) porque no contiene AEBSF, lo cual puede provocar que los picos se desplacen.
• Validado: totalmente compatible con los tampones de lisis celular Thermo Scientific Pierce.

El cóctel de inhibidores de proteasas y fosfatasas Halt está específicamente optimizado para proteger las proteínas de la degradación durante la extracción y purificación. El cóctel contiene inhibidores contra las principales clases de proteasas y fosfatasas, por lo que se ocupa de aminopeptidasas, proteasas de cisteína y serina, y fosfatasas de serina/treonina y proteína tirosina.

Productos relacionados
Cóctel de inhibidores de proteasa y fosfatasa Halt™ (100X)

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
TampónTampón de lisis
DescripciónCóctel inhibidor de proteasa y fosfatasa Halt, sin EDTA
InhibidoresInhibidor de la proteasa, inhibidor de la fosfatasa
Cantidad10 ml
Tipo de reactivoInhibidor de la proteasa, inhibidor de la fosfatasa
FormularioLíquido
Línea de productosHalt
Tipo de productoCóctel inhibidor
Unit SizeEach
Contenido y almacenamiento
Tras su recepción a 4 °C. Producto enviado con un paquete de hielo. No congele el producto.

Preguntas frecuentes

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™ FAQs

Citations & References (6)

Citations & References
Abstract
2-Aminopyridine-Based Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) Inhibitors: Assessment of Mechanism-Based Safety.
Authors:Dow RL,Ammirati M,Bagley SW,Bhattacharya SK,Buckbinder L,Cortes C,El-Kattan AF,Ford K,Freeman GB,Guimarães CRW,Liu S,Niosi M,Skoura A,Tess D
Journal:Journal of medicinal chemistry
PubMed ID:29570292
Studies have linked the serine-threonine kinase MAP4K4 to the regulation of a number of biological processes and/or diseases, including diabetes, cancer, inflammation, and angiogenesis. With a majority of the members of our lead series (e.g., 1) suffering from time-dependent inhibition (TDI) of CYP3A4, we sought design avenues that would eliminate ... More
Deficiency of endothelial sirtuin1 in mice stimulates skeletal muscle insulin sensitivity by modifying the secretome.
Authors:Li Q,Zhang Q,Kim YR,Gaddam RR,Jacobs JS,Bachschmid MM,Younis T,Zhu Z,Zingman L,London B,Rauckhorst AJ,Taylor EB,Norris AW,Vikram A,Irani K
Journal:Nature communications
PubMed ID:37696839
Downregulation of endothelial Sirtuin1 (Sirt1) in insulin resistant states contributes to vascular dysfunction. Furthermore, Sirt1 deficiency in skeletal myocytes promotes insulin resistance. Here, we show that deletion of endothelial Sirt1, while impairing endothelial function, paradoxically improves skeletal muscle insulin sensitivity. Compared to wild-type mice, male mice lacking endothelial Sirt1 (E-Sirt1-KO) ... More
Mito-Mendelian interactions alter in vivo glucose metabolism and insulin sensitivity in healthy mice.
Authors:Sammy MJ,Connelly AW,Brown JA,Holleman C,Habegger KM,Ballinger SW
Journal:American journal of physiology. Endocrinology and metabolism
PubMed ID:34370595
The regulation of euglycemia is essential for human health with both chronic hypoglycemia and hyperglycemia having detrimental effects. It is well documented that the incidence of type 2 diabetes increases with age and exhibits racial disparity. Interestingly, mitochondrial DNA (mtDNA) damage also accumulates with age and its sequence varies with ... More
Deciphering the interactome of Ataxin-2 and TDP-43 in iPSC-derived neurons for potential ALS targets.
Authors:Tian Y,Heinsinger N,Hu Y,Lim UM,Wang Y,Fernandis AZ,Parmentier-Batteur S,Klein B,Uslaner JM,Smith SM
Journal:PloS one
PubMed ID:39739690
Ataxin-2 is a protein containing a polyQ extension and intermediate length of polyQ extensions increases the risk of Amyotrophic Lateral Sclerosis (ALS). Down-regulation of Ataxin-2 has been shown to mitigate TDP-43 proteinopathy in ALS models. To identify alternative therapeutic targets that can mitigate TDP-43 toxicity, we examined the interaction between ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
View all Cóctel inhibidor de proteasas y fosfatasas Halt sin EDTA (100X) Halt™ citations