NPE-caged ATP (Adenosine 5'-Triphosphate, P3-(1-(2-Nitrophenyl)Ethyl) Ester, Disodium Salt)
NPE-caged ATP (Adenosine 5'-Triphosphate, <i>P</i><sup>3</sup>-(1-(2-Nitrophenyl)Ethyl) Ester, Disodium Salt)
Citas y referencias (132)
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NPE-caged ATP (Adenosine 5'-Triphosphate, P3-(1-(2-Nitrophenyl)Ethyl) Ester, Disodium Salt)

El ATP enjaulado con NPE es un análogo de nucleótido en el que el fosfato terminal se esterifica con unMás información
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Número de catálogoCantidad
A1048
también denominado A-1048
5 mg
Número de catálogo A1048
también denominado A-1048
Precio (CLP)
-
Cantidad:
5 mg
El ATP enjaulado con NPE es un análogo de nucleótido en el que el fosfato terminal se esterifica con un grupo de bloqueo, lo que hace que la molécula sea biológicamente inactiva. La fotólisis flash del grupo enjaulado con iluminación UV a 360 nm provoca una liberación rápida y altamente localizada del nucleótido libre en el sitio de iluminación.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Etiqueta o tinteNPE (éster P3-(1-(2-nitrofenil)etil))
Tipo de productoATP enjaulado con NPE
Cantidad5 mg
Condiciones de envíoHielo húmedo
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Citations & References (132)

Citations & References
Abstract
Authors:
Journal:
PubMed ID:8980790
Sliding distance per ATP molecule hydrolyzed by myosin heads during isotonic shortening of skinned muscle fibers.
Authors:Higuchi H, Goldman YE
Journal:Biophys J
PubMed ID:8534820
'We measured isotonic sliding distance of single skinned fibers from rabbit psoas muscle when known and limited amounts of ATP were made available to the contractile apparatus. The fibers were immersed in paraffin oil at 20 degrees C, and laser pulse photolysis of caged ATP within the fiber initiated the ... More
Rotational dynamics of actin-bound intermediates in the myosin ATPase cycle.
Authors:Berger CL, Thomas DD
Journal:Biochemistry
PubMed ID:1657157
'We have used saturation-transfer electron paramagnetic resonance (ST-EPR) to detect the microsecond rotational motions of spin-labeled myosin subfragment one (MSL-S1) bound to actin in the presence of the ATP analogues AMPPNP (5''-adenylylimido diphosphate) and ATP gamma S [adenosine 5''-O-(3-thiotriphosphate)], which are believed to trap myosin in strongly and weakly bound ... More
Sodium pump-mediated ATP:ADP exchange. The sided effects of sodium and potassium ions.
Authors:Kaplan JH
Journal:J Gen Physiol
PubMed ID:6294224
'Resealed human red cell ghosts containing caged ATP (Kaplan et al., 1978) and [3H]ADP were irradiated at 340 nm. The photochemical release of free ATP initiated a rapid transphosphorylation reaction (ATP:ADP exchange), a component of which is inhibited by ouabain. The reaction rate was measured by following the rate of ... More
Na+ movement in a single turnover of the Na pump.
Authors:Forbush B
Journal:Proc Natl Acad Sci U S A
PubMed ID:6089192
'Ouabain-sensitive 22Na efflux from right-side-out membrane vesicles prepared from dog kidney has been examined with a time resolution of 30 msec. The vesicles are preloaded with 22Na and caged ATP [P3-1-(2-nitro)phenylethyl adenosine triphosphate], so that transport by the Na pump can be initiated by light. After a brief illumination, which ... More
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