Phalloidin Labeling Probes
Phalloidin Labeling Probes
Citas y referencias (85)
Invitrogen™

Phalloidin Labeling Probes

Achieve precise and reliable F-actin staining with fluorescent and biotinylated phalloidins. Phalloidin conjugates are widely used in imaging applications to selectively label F-actin in a variety of sample types including fixed and permeabilized cells, tissue sections, and cell-free experiments.
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Número de catálogoColorIntervalo de longitud de onda de excitaciónTipo de colorante
A12380Rojo anaranjado578⁄600Alexa Fluor™ 568
A22281Azul346⁄442Alexa Fluor™ 350
A30104Violeta405/450Alexa Fluor™ Plus 405
A12379Verde495⁄518Alexa Fluor™ 488
O7466Verde496⁄520Oregon Green™ 488
F432Verde496⁄516FITC (fluoresceína)
A22282Amarillo531⁄554Alexa Fluor™ 532
R415Rojo anaranjado540⁄565TRITC (isotiocianato de tetrametilrodamina)
A22283Naranja556⁄570Alexa Fluor™ 546
A34055Naranja555⁄565Alexa Fluor™ 555
A30106Naranja555/565 nmAlexa Fluor Plus 555
B3475Rojo558⁄569BODIPY™
A12381Rojo581⁄609Alexa Fluor™ 594
T7471Rojo591⁄608Texas Red™
A22284Rojo lejano632⁄647Alexa Fluor™ 633
A34054Rojo lejano633⁄647Alexa Fluor™ 635
A22287Rojo lejano650⁄668Alexa Fluor™ 647
A30107Rojo lejano650/668 nmAlexa Fluor Plus 647
A22285Infrarrojo cercano663⁄690Alexa Fluor™ 660
A22286Infrarrojo cercano679⁄702Alexa Fluor™ 680
A30105Infrarrojo cercano758/784Alexa Fluor™ Plus 750
B7474NingunoNingunoBiotin-XX
P3457NingunoNingunoFaloidina (sin marcar)
Número de catálogo A12380
Precio (CLP)
607.921
Each
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Color:
Rojo anaranjado
Intervalo de longitud de onda de excitación:
578⁄600
Tipo de colorante:
Alexa Fluor™ 568
Precio (CLP)
607.921
Each
Añadir al carro de la compra
Fluorescent and biotinylated phalloidins are water soluble and bind to filamentous actin (F-actin) with nanomolar affinity, making them convenient probes for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections, fixed and permeabilized cells, and cell-free experiments. Phalloidin conjugates bind similarly to actin from various species, including plants and animals, enabling staining of the cytoskeleton in a wide range of samples.

A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.

Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.

Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining

Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.

Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.

Features of phalloidin probes

  • High specificity—binds selectively to F-actin, which allows for precise labeling of actin filaments in fixed cells and cryopreserved tissues
  • Strong affinity—nanomolar binding affinity for F-actin, which ensures stable and reliable actin staining
  • Extensive fluorescent conjugate options—over twenty conjugated varieties of phalloidin
  • Compatibility with fixed samples—typically used with fixed cells and tissues, making them suitable for actin staining in detailed structural studies, immunofluorescence staining, and IHC applications
  • Multiplexing capability—the wide availability of phalloidin conjugates enables their use in combination with other fluorescent probes and antibodies for multiplex imaging. Biotinylated phalloidin can be made use of in downstream streptavidin steps.
  • Quantitative analysis—can be used for quantitative analysis of F-actin distribution and density within cells, aiding in the study of cytoskeletal dynamics. The unlabeled phalloidin can be titrated as a control.
  • Ease of use—staining is straightforward and quick
  • Excellent stability—exhibit good photostability, which is essential for prolonged imaging sessions and time-lapse studies
  • Wide applicability—used for a range of applications, including studying cell morphology, motility, and the effects of drugs on the actin cytoskeleton

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
ColorRojo anaranjado
Tipo de coloranteAlexa Fluor™ 568
Intervalo de longitud de onda de excitación578⁄600
Para utilizar con (equipo)Fluorescence Microscope, Flow Cytometer, Confocal Microscope, Compatible with Texas Red filter set
Línea de productosAlexa Fluor
Cantidad300 Units
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaColorantes Alexa Fluor
Tipo de productoFaloidina
SubCellular LocalizationActina, citoesqueleto, Cytoskeleton
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.

Preguntas frecuentes

Can I combine Click-iT or Click-iT Plus reactions with phalloidin conjugates used for actin staining?

We do not recommend using phalloidin conjugates for staining actin in combination with traditional Click-iT or Click-iT Plus reactions since phalloidin is extremely sensitive to the presence of copper.

For staining actin in combination with traditional Click-iT or Click-iT Plus reactions, we recommend using anti-α-actin antibodies for staining actin in the cytoskeleton. You can find a list of our actin antibodies here.

Another option would be to use the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Cat. Nos. C10641, C10642, C10643). These Click-iT Plus toolkits provide Copper and Copper protectant separately which makes it easier to titrate the copper concentration to obtain optimal labeling with minimal copper-mediated damage. You may need to optimize the click reaction with the lowest possible concentration of copper and then perform the phalloidin staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to label my paraffin sections for F-actin with a phalloidin conjugate, but I'm not seeing any signal. Why?

When cells and tissues are treated with solvents such as xylene or acetone (for example during deparaffinization of tissue sections), it affects the F-actin in a way that prevents phalloidins from binding. Phalloidin may be used with cryosections, which are not typically washed with organic solvents, or anti-actin antibodies may be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (85)

Citations & References
Abstract
Control of integrin alphaIIb beta3 outside-in signaling and platelet adhesion by sensing the physical properties of fibrin(ogen) substrates.
Authors:Podolnikova NP, Yermolenko IS, Fuhrmann A, Lishko VK, Magonov S, Bowen B, Enderlein J, Podolnikov AV, Ros R, Ugarova TP,
Journal:Biochemistry
PubMed ID:19929007
The physical properties of substrates are known to control cell adhesion via integrin-mediated signaling. Fibrin and fibrinogen, the principal components of hemostatic and pathological thrombi, may represent biologically relevant substrates whose variable physical properties control adhesion of leukocytes and platelets. In our previous work, we have shown that binding of ... More
Phosphocaveolin-1 is a mechanotransducer that induces caveola biogenesis via Egr1 transcriptional regulation.
Authors:Joshi B, Bastiani M, Strugnell SS, Boscher C, Parton RG, Nabi IR,
Journal:J Cell Biol
PubMed ID:23091071
'Caveolin-1 (Cav1) is an essential component of caveolae whose Src kinase-dependent phosphorylation on tyrosine 14 (Y14) is associated with regulation of focal adhesion dynamics. However, the relationship between these disparate functions remains to be elucidated. Caveola biogenesis requires expression of both Cav1 and cavin-1, but Cav1Y14 phosphorylation is dispensable. In ... More
Micron-scale spatially patterned, covalently immobilized vascular endothelial growth factor on hydrogels accelerates endothelial tubulogenesis and increases cellular angiogenic responses.
Authors:Leslie-Barbick JE, Shen C, Chen C, West JL,
Journal:Tissue Eng Part A
PubMed ID:20712418
'Spontaneous formation of endothelial tubules was restricted to patterned micron-scale regions presenting cell adhesion ligands and angiogenic signaling protein on poly(ethylene glycol) hydrogels. Arginine-glycine-aspartic acid-serine (RGDS), an integrin ligand, and vascular endothelial growth factor (VEGF), a rate-limiting signaling protein involved in angiogenesis, were covalently bound through photopolymerization via laser scanning ... More
Phosphorylation of spinophilin by ERK and cyclin-dependent PK 5 (Cdk5).
Authors:Futter M, Uematsu K, Bullock SA, Kim Y, Hemmings HC, Nishi A, Greengard P, Nairn AC
Journal:Proc Natl Acad Sci U S A
PubMed ID:15728359
'Spinophilin is a protein that binds to protein phosphatase-1 and actin and modulates excitatory synaptic transmission and dendritic spine morphology. We have identified three sites phosphorylated by ERK2 (Ser-15 and Ser-205) and cyclin-dependent PK 5 (Cdk5) (Ser-17), within the actin-binding domain of spinophilin. Cdk5 and ERK2 both phosphorylated spinophilin in ... More
A high-throughput, cell-based screening method for siRNA and small molecule inhibitors of mTORC1 signaling using the In Cell Western technique.
Authors:Hoffman GR, Moerke NJ, Hsia M, Shamu CE, Blenis J,
Journal:Assay Drug Dev Technol
PubMed ID:20085456
'The mTORC1 pathway is a central regulator of cell growth, and defective mTORC1 regulation plays a causative role in a variety of human diseases, including cancer, tumor syndromes such as the tuberous sclerosis complex (TSC) and lymphangioleiomyomatosis (LAM), and metabolic diseases such as diabetes and obesity. Given the importance of ... More
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