DMEM/F-12 CTS™ KnockOut™ GIBCO

Citas y referencias (1)

Gibco™

DMEM/F-12 CTS™ KnockOut™ GIBCO

DMEM/F-12 CTS™ KnockOut™ Cell Therapy Systems es un medio sin suero, de baja osmolalidad sin L-glutamina o tampón HEPES optimizadoMás información

Número de catálogo	Cantidad
A1370801	500 mL

Número de catálogo A1370801

Precio (CLP)

78.177

Añadir al carro de la compra

Cantidad:

500 mL

Precio (CLP)

78.177

Añadir al carro de la compra

DMEM/F-12 CTS™ KnockOut™ Cell Therapy Systems es un medio sin suero, de baja osmolalidad sin L-glutamina o tampón HEPES optimizado para el crecimiento de células madre embrionarias (ESC) y células madre pluripotentes (iPSC) inducidas humanas. El medio DMEM/F-12 CTS™ KnockOut™ está diseñado para su uso con los suplementos KnockOut™ SR y KnockOut™ SR sin xenógenos CTS™.

Diseñado para ofrecer un rendimiento fiable y uniforme.

• Realice cultivos de ESC/iPSC con poca o ninguna diferenciación espontánea.
• Prepárese para la investigación clínica con esta fórmula fabricada de conformidad con las prácticas recomendadas de fabricación actuales y con documentación de apoyo.

Resultados uniformes sin suero bovino fetal.
El medio DMEM/F-12 CTS™ KnockOut™ Cell Therapy Systems forma parte de la línea de medios KnockOut™ que han proporcionado resultados fiables para el cultivo de ESC y ahora iPSC durante años. Estos medios sin suero bovino fetal dan lugar a poca o ninguna diferenciación espontánea y evita que tenga que llevar a cabo cribajes repetidos.

Morfología equivalente
Las células madre cultivadas en medios DMEM/F-12 CTS™ KnockOut™ muestran una morfología y un cariotipo normales en comparación con las células madre de control. La morfología es equivalente a las hESC pasadas manualmente, incluso después del pasaje enzimático.

Simplifique su transición a la investigación clínica
La marca Gibco™ Cell Therapy Systems (CTS™) ofrece una amplia gama de productos para emplear en aplicaciones de terapia celular, incluidos medios, reactivos, factores de crecimiento, enzimas, reactivos de selección/expansión de células y dispositivos. Los productos Gibco™ Cell Therapy Systems (CTS™) ofrecen:
• Menor carga de cualificar los reactivos.
• Certificados adecuados de análisis y de origen.
• Acceso a nuestro archivo principal sobre fármacos (DMF).

Para su uso en investigación o fabricación de productos basados en células, genes o tejidos. PRECAUCIÓN: no diseñado para la administración directa en seres humanos o animales.

Especificaciones

Tipo de célulaCélulas madre (embrionarias), células madre (iPS, pluripotentes inducidas)

Para utilizar con (aplicación)Cell and Gene Therapy Research, Development, and Manufacturing

FormatoFrasco

Calidad de fabricaciónBuenas prácticas de fabricación actuales para dispositivos médicos, 21 CFR Parte 820 e ISO 13485

Tipo de productoDMEM/F-12 CTS KnockOut

Cantidad500 mL

Duración de almacenamiento18 months from date of manufacture

Condiciones de envíoAmbiente

ClasificaciónLibre de material de origen animal

FormularioLíquido

EsterilidadEstéril con filtro

Unit SizeEach

Contenido y almacenamiento

Almacenar entre 2 C y 8 °C y proteger de la luz.

Preguntas frecuentes

What is CTS?

The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer DMEM/F12 medium without folic acid?

We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all DMEM/F-12 CTS™ KnockOut™ GIBCO FAQs

Citations & References (1)

Citations & References

Abstract

cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness.

Authors:Wiley LA, Burnight ER, DeLuca AP, Anfinson KR, Cranston CM, Kaalberg EE, Penticoff JA, Affatigato LM, Mullins RF, Stone EM, Tucker BA,

Journal:Sci Rep

PubMed ID:27471043

'Immunologically-matched, induced pluripotent stem cell (iPSC)-derived photoreceptor precursor cells have the potential to restore vision to patients with retinal degenerative diseases like retinitis pigmentosa. The purpose of this study was to develop clinically-compatible methods for manufacturing photoreceptor precursor cells from adult skin in a non-profit cGMP environment. Biopsies were obtained ... More