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Citas y referencias (106)
Invitrogen™
Nonyl Acridine Orange (Acridine Orange 10-Nonyl Bromide)
La naranja de acridina está bien retenida en las mitocondrias, lo que la convierte en una sonda útil para seguirMás información
| Número de catálogo | Cantidad |
|---|---|
| A1372 | 100 mg |
Número de catálogo A1372
Precio (CLP)
153.466
Each
Cantidad:
100 mg
Precio (CLP)
153.466
Each
La naranja de acridina está bien retenida en las mitocondrias, lo que la convierte en una sonda útil para seguir a las mitocondrias durante el aislamiento y después de la fusión celular. Se informa que la captación mitocondrial no depende del potencial de membrana, a diferencia de muchas otras sondas mitocondriales. Es tóxico en altas concentraciones y aparentemente se une a la cardiopilina en todas las mitocondrias, independientemente de su estado energético. Este derivado se ha utilizado para analizar las mitocondrias mediante citometría de flujo, caracterizar la resistencia multifarmacológica y medir los cambios en la masa mitocondrial durante la apoptosis.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorNaranja
Método de detecciónFluorescente
Para utilizar con (equipo)Microscopio de fluorescencia
Cantidad100 mg
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaFluorescent Dye
Tipo de productoNaranja de nonil acridina
SubCellular LocalizationMitocondrias
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.
Citations & References (106)
Citations & References
Abstract
Analysis of mitochondria isolated from single cells.
Journal:Analytical and bioanalytical chemistry
PubMed ID:16937092
Human epidermal cells progressively lose their cardiolipins during ageing without change in mitochondrial transmembrane potential.
Journal:Mech Ageing Dev
PubMed ID:7745994
Mitochondria dysfunction is considered to be a major cause of the modifications that occur during cell ageing. For this reason, cardiolipin, a suitable marker of the chondriome, as well as the mitochondrial transmembrane potential were examined in keratinocytes obtained from 9- to 75-year-old women. The study was carried out by
Tamoxifen but not 4-hydroxytamoxifen initiates apoptosis in p53(-) normal human mammary epithelial cells by inducing mitochondrial depolarization.
Journal:J Biol Chem
PubMed ID:11094056
'Despite the widespread clinical use of tamoxifen as a breast cancer prevention agent, the molecular mechanism of tamoxifen chemoprevention is poorly understood. Abnormal expression of p53 is felt to be an early event in mammary carcinogenesis. We developed an in vitro model of early breast cancer prevention to investigate how
Sortase A localizes to distinct foci on the Streptococcus pyogenes membrane.
Journal:Proc Natl Acad Sci U S A
PubMed ID:19017791
'Cell wall peptidoglycan-anchored surface proteins are essential virulence factors in many gram-positive bacteria. The attachment of these proteins to the peptidoglycan is achieved through a transpeptidation reaction, whereby sortase cleaves a conserved C-terminal LPXTG motif and covalently attaches the protein to the peptidoglycan precursor lipid II. It is unclear how
Methods for assessing autophagy and autophagic cell death.
Journal:Methods Mol Biol
PubMed ID:18425442
'Autophagic (or type 2) cell death is characterized by the massive accumulation of autophagic vacuoles (autophagosomes) in the cytoplasm of cells that lack signs of apoptosis (type 1 cell death). Here we detail and critically assess a series of methods to promote and inhibit autophagy via pharmacological and genetic manipulations.