TaqMan™ hPSC Scorecard™ Panel, 384-well
TaqMan™ hPSC Scorecard™ Panel, 384-well
Citas y referencias (20)
Applied Biosystems™

TaqMan™ hPSC Scorecard™ Panel, 384-well

El panel Scorecard™ de hPSC TaqMan™ Applied Biosystems™ de 384 w permite la verificación de pluripotencia y la determinación delMás información
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Número de catálogoCantidad
A158701 x 384-well plate
Número de catálogo A15870
Precio (CLP)
-
Cantidad:
1 x 384-well plate
El panel Scorecard™ de hPSC TaqMan™ Applied Biosystems™ de 384 w permite la verificación de pluripotencia y la determinación del sesgo de linaje para las líneas celulares humanas ES e IPS. La placa de 384 pocillos contiene cuatro conjuntos de 94 ensayos predefinidos de expresión génica TaqMan™ (incluidos los controles endógenos) secos en los pocillos. La mezcla maestra no se incluye con este panel. Para ver un kit que combina el panel con la mezcla maestra, consulte Kit Scorecard™ de HPSC TaqMan™ de 384 w. Este producto también está disponible en un formato rápido de 2 x 96 w.

• Evalúe la firma génica de cuatro muestras en un solo experimento
• Compare los resultados con un estándar de referencia con el software de análisis adjunto Scorecard™ de hPSC
• Identifique líneas con potencial de diferenciación de trilinaje
• Evalúe la diferenciación inductiva especificada en una capa germinal
• Acceda a contenido validado para aumentar la confianza en sus resultados

Evalúe toda una firma génica en un experimento
El panel Scorecard™de hPSC TaqMan™ ayuda a ahorrar tiempo al ofrecer ensayos preplacados en un formato seco estable y conveniente. Solo tiene que añadir la mezcla maestra TaqMan™ a su ADNc de interés y transferirlo a la placa de 384 pocillos con una pipeta multicanal.

Compare los resultados con un estándar de referencia
El panel Scorecard™ de hPSC TaqMan™ incluye acceso a software de análisis basado en la nube que le permite ver y exportar gráficos y tablas de resultados, así como generar informes. El software es compatible con siete sistemas qRT-PCR Applied Biosystems™ y está disponible sin coste adicional.

Identifique líneas con potencial de diferenciación trilineal
Incluya muestras de cuerpos embrioides (EB) diferenciadas de manera aleatoria (diferenciadas durante al menos 7 días utilizando métodos estándar de EB) en su análisis y determine si sus líneas están sesgadas hacia una o más de las tres capas germinales (endodermo, mesodermo, ectodermo).

Evalúe la diferenciación inductiva especificada en una capa germinal
El panel incluye más de veinte marcadores para cada una de las tres capas de germen que han demostrado responder como se esperaba a la diferenciación dirigida en el cultivo de monocapa. Evalúe rápidamente los datos de los ciclos de tiempo, las condiciones de cultivo y las formulaciones de medios para su capa germinal de interés con este panel único.

Acceso a contenido validado
Los contenidos del panel se basan en trabajos publicados (Bock et al., Cell 144, 439–452, 2011) y fueron validados según múltiples líneas de ES e IPS humanas.

Lo que se obtiene
El panel Scorecard™ de hPSC TaqMan™ de 384w incluye lo siguiente:

• Una placa de 384 pocillos de 94 ensayos de expresión génica TaqMan™ (4 juegos de genes por placa)
• Una cubierta de placa óptica
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Para utilizar con (equipo)QuantStudio™ 12k Flex, Sistema ViiA™ 7
FormatoPlaca de 384 pocillos
N.º de reacciones384 reacciones
Línea de productosTaqMan
Cantidad1 x 384-well plate
Categoría de investigaciónInvestigación con células madre
EspecieHumano
Método de detecciónSonda de cebado
Para utilizar con (aplicación)Análisis de células madre
FormularioSecado
Velocidad de reacciónEstándar
Unit SizeEach
Contenido y almacenamiento
Contenido:Una placa de 384 pocillos con cuatro repeticiones del panel de 96 ensayos y una cubierta óptica

Almacenar a temperatura ambiente.

Preguntas frecuentes

Will I need to update my TaqMan hPSC Scorecard Analysis Software?

No software update is required. Because the hPSC Scorecard Analysis Software is cloud based, you will always have access to the most up-to-date version of the software every time you log in.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center

How do I export Ct values in the TaqMan hPSC Scorecard Analysis Software?

The only way to view the Ct values in the current version is through the expression plot. We expect the software's Excel export to include the gene names and Ct values in a future version of the software.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center

I'm using the TaqMan hPSC Scorecard Analysis Software. How are the scores calculated?

A proprietary algorithm compares the Ct values for each marker set to the values in the reference database and calculates the score based on how well the expression correlates. In general, scores close to 0 indicate comparable expression to that of the reference standard using undifferentiated cells. Scores higher than 1 indicates up regulation relative to undifferentiated cells and less than -1 indicate down regulation relative to undifferentiated cells.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center

I'm using the TaqMan hPSC Scorecard Analysis Software. What lines were used to generate the reference standard?

Thirteen pluripotent stem cell lines were used to generate the reference standard including:

  • H9 ESC P28
  • iPS BS3C P35
  • iPS18C P29
  • HUES9 P28
  • HUES13 P56
  • HUES28 P28
  • HUES44 P25
  • HUES48 P21
  • HUES49 P21
  • HUES53 P24
  • HUES63 P46
  • HUES64 P30
  • HUES65 P29


Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center

I'm using the TaqMan hPSC Scorecard Analysis Software. My sample has a red flag on it, or is not showing any results. What does that mean?

The hPSC Scorecard Analysis Software performs a set of quality control checks upon data import to ensure the quality of the results.
You may see one of the following red flag warnings:

  • Internal positive control: The expression levels of the housekeeping genes were low, signifying that there was an issue with the qRT-PCR. The sample is excluded from analysis and must be repeated.
  • Bad Rox: The ROX dye is used as a passive reference during the qPCR. If the ROX dye is not detected, it is suggestive that some of the assay wells may not have received the proper amount of Master Mix. This may explain why a particular assay is not showing the anticipated gene expression level.
  • Sendai Virus Detected (SEV): The hPSC Scorecard Panel includes a control to look for Sendai virus expression. A flag will appear if a Ct value of greater than 30 is observed for this control. On some rare occasions, the SEV oligonucleotides will cross react with other genes resulting in a false SEV flag in differentiated cells. If you see this flag, and did not anticipate Sendai virus detection, you may want to repeat the sample or look at the amplification plot (In the 96 well plate: well B9; in the 384 well plate: wells D3, D9, D15, and D21) to see if the automated Ct value call is real, or just an error.
  • Insufficient Data: The analysis software requires a minimum number of genes to be amplified in each the four categories (i.e., self-renewal (“pluri”), ectoderm, mesoderm, and endoderm) to accurately analyze your data. If the minimum number of genes in each category does not amplify, or if data has been omitted by the user, the Insufficient Data flag will appear and the sample is excluded from analysis. The sample should be repeated.


Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center

View all TaqMan™ hPSC Scorecard™ Panel, 384-well FAQs

Citations & References (20)

Citations & References
Abstract
Characterizing Pluripotent Stem Cells Using the TaqMan(®) hPSC Scorecard (TM) Panel.
Authors:Fergus J, Quintanilla R, Lakshmipathy U,
Journal:
PubMed ID:25138722
'Rapid technological developments for the efficient generation of footprint-free induced pluripotent stem cells (iPSC) enabled the creation of patient-specific iPSC for downstream applications in drug discovery and regenerative medicine. However, the large number of iPSCs, generated from diverse genetic backgrounds using various methods and culture conditions, created a steep challenge ... More
Generation of iPSCs as a Pooled Culture Using Magnetic Activated Cell Sorting of Newly Reprogrammed Cells.
Authors:Yang W, Liu Y, Slovik KJ, Wu JC, Duncan SA, Rader DJ, Morrisey EE,
Journal:
PubMed ID:26281015
'Although significant advancement has been made in the induced pluripotent stem cell (iPSC) field, current methods for iPSC derivation are labor intensive and costly. These methods involve manual selection, expansion, and characterization of multiple clones for each reprogrammed cell sample and therefore significantly hampers the feasibility of studies where a ... More
Efficient Generation of Induced Pluripotent Stem and Neural Progenitor Cells From Acutely Harvested Dura Mater Obtained During Ventriculoperitoneal Shunt Surgery.
Authors:Cary WA, Hori CN, Pham MT, Nacey CA, McGee JL, Hamou M, Berman RF, Bauer G, Nolta JA, Waldau B,
Journal:
PubMed ID:26074438
'The dura mater can be easily biopsied during most cranial neurosurgical operations. We describe a protocol that allows for robust generation of induced pluripotent stem cells (iPSCs) and neural progenitors from acutely harvested dura mater. To generate iPSCs and neural progenitor cells from dura mater obtained during ventriculoperitoneal shunt surgery. ... More
Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of Induced Pluripotency.
Authors:Cacchiarelli D, Trapnell C, Ziller MJ, Soumillon M, Cesana M, Karnik R, Donaghey J, Smith ZD, Ratanasirintrawoot S, Zhang X, Ho Sui SJ, Wu Z, Akopian V, Gifford CA, Doench J, Rinn JL, Daley GQ, Meissner A, Lander ES, Mikkelsen TS,
Journal:
PubMed ID:26186193
'Induced pluripotency is a promising avenue for disease modeling and therapy, but the molecular principles underlying this process, particularly in human cells, remain poorly understood due to donor-to-donor variability and intercellular heterogeneity. Here, we constructed and characterized a clonal, inducible human reprogramming system that provides a reliable source of cells ... More
Transcription factor binding dynamics during human ES cell differentiation.
Authors:Tsankov AM, Gu H, Akopian V, Ziller MJ, Donaghey J, Amit I, Gnirke A, Meissner A,
Journal:
PubMed ID:25693565
'Pluripotent stem cells provide a powerful system to dissect the underlying molecular dynamics that regulate cell fate changes during mammalian development. Here we report the integrative analysis of genome-wide binding data for 38 transcription factors with extensive epigenome and transcriptional data across the differentiation of human embryonic stem cells to ... More
View all TaqMan™ hPSC Scorecard™ Panel, 384-well citations