Reactivo Amplex™ Red
Reactivo Amplex™ Red
Citas y referencias (224)
Invitrogen™

Reactivo Amplex™ Red

El reactivo Amplex Red, una sonda altamente sensible y estable para el H2O2, es nuestro mejor sustrato fluorogénico para laMás información
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Número de catálogoCantidadTipo de colorante
A2217710 x 10 mgReactivo Amplex Red
A122225 mgReactivo Amplex Red
A360065 x 1 mgReactivo Amplex UltraRed
Número de catálogo A22177
Precio (CLP)
3.379.085
Each
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Cantidad:
10 x 10 mg
Tipo de colorante:
Reactivo Amplex Red
Precio (CLP)
3.379.085
Each
Añadir al carro de la compra
El reactivo Amplex Red, una sonda altamente sensible y estable para el H2O2, es nuestro mejor sustrato fluorogénico para la peroxidasa. En presencia de peroxidasa de rábano (HRP), el reactivo Amplex Red reacciona en una estequiometría de 1:1 con H2O2 para producir resorufina altamente fluorescente. Dado que el H2O2 se produce en muchas reacciones enzimáticas diferentes, el reactivo Amplex Red puede usarse para detectar la actividad de muchas enzimas diferentes.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Método de detecciónFluorescente
Tipo de coloranteReactivo Amplex Red
FormularioSólido
Cantidad10 x 10 mg
Condiciones de envíoTemperatura ambiente
SustratoHRP (Horseradish Peroxidase) Substrate
Tipo de sustratoChemical substrate
Aplicación validadaCell Analysis
Para utilizar con (aplicación)Hydrogen Peroxidase Assay
Para utilizar con (equipo)Fluorometer, Microplate Reader
Línea de productosAmplex
Tipo de productoRed Hydrogen Peroxide
Unit SizeEach
Contenido y almacenamiento
Almacenar en congelador (de - 5 a - 30 °C).

Preguntas frecuentes

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have ordered and tested your Amplex Red Reagent (Cat. Nos. A12222, A22177), but it is not reacting correctly. What could be the cause?

Either the horseradish peroxidase or other enzymes in the assay may not be active, the H2O2 may have degraded, or the sample could have some component that is consuming H2O2 or inactivating the enzymes required for the assay. We recommend that you check when the reagent was purchased and how it was stored prior to use; the reagent should not be used past the warranty period.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you recommend using Amplex Red Reagent (Cat. Nos. A12222, A22177) in live cells?

Amplex Red is cell impermeant, but also its conversion to resorufin requires direct contact with the reaction of peroxidase with H2O2 (in solution, at the surface, or within cells). Within cells, peroxidases and H2O2 may not be sufficiently localized or active to provide enough signal.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use Amplex Red Reagent (Cat. Nos. A12222, A22177) in the presence of catalases?

Unless you are specifically analyzing catalase activity, as with the Amplex Red Catalase Assay Kit (Cat. No. A22180), the assay should be devoid of catalases. Catalases compete with the horseradish peroxidase for H2O2; the conversion of Amplex Red to resorufin requires the reaction of horseradish peroxidase with H2O2.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I store Amplex Red Reagent (Cat. Nos. A12222, A22177)?

We recommend storing Amplex Red Reagent under vacuum (anaerobic conditions) and dehydrated. Do not store Amplex Red Reagent in solution.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Reactivo Amplex™ Red FAQs

Citations & References (224)

Citations & References
Abstract
Roles for the human ATP-dependent Lon protease in mitochondrial DNA maintenance.
Authors:Lu B,Yadav S,Shah PG,Liu T,Tian B,Pukszta S,Villaluna N,Kutejová E,Newlon CS,Santos JH,Suzuki CK
Journal:The Journal of biological chemistry
PubMed ID:17420247
Pressure overload-induced mild cardiac hypertrophy reduces left ventricular transmural differences in mitochondrial respiratory chain activity and increases oxidative stress.
Authors:Kindo M, Gerelli S, Bouitbir J, Charles AL, Zoll J, Hoang Minh T, Monassier L, Favret F, Piquard F, Geny B,
Journal:Front Physiol
PubMed ID:22934079
Objective: Increased mechanical stress and contractility characterizes normal left ventricular (LV) subendocardium (Endo) but whether Endo mitochondrial respiratory chain complex activities is reduced as compared to subepicardium (Epi) and whether pressure overload-induced LV hypertrophy (LVH) might modulate transmural gradients through increased reactive oxygen species (ROS) production is unknown. Methods: LVH ... More
Overexpression of nicastrin increases Abeta production.
Authors:Murphy MP, Das P, Nyborg AC, Rochette MJ, Dodson MW, Loosbrock NM, Souder TM, McLendon C, Merit SL, Piper SC, Jansen KR, Golde TE
Journal:FASEB J
PubMed ID:12692078
Gamma-secretase cleavage is the final proteolytic step that releases the amyloid beta-peptide (Abeta) from the amyloid beta-protein precursor (APP). Significant evidence indicates that the presenilins (PS) are catalytic components of a high molecular weight gamma-secretase complex. The glycoprotein nicastrin was recently identified as a functional unit of this complex based ... More
Association of excitatory amino acid transporters, especially EAAT2, with cholesterol-rich lipid raft microdomains: importance for excitatory amino acid transporter localization and function.
Authors:Butchbach ME, Tian G, Guo H, Lin CL
Journal:J Biol Chem
PubMed ID:15187084
In the present study, we investigated the role of membrane cholesterol in the function of glutamate transporters. Depletion of membrane cholesterol by methyl-beta-cyclodextrin resulted in reduced Na(+)-dependent glutamate uptake in primary cortical cultures. Glial glutamate transporter EAAT2-mediated uptake was more sensitive to this effect. Cell surface biotinylation and immunostaining experiments ... More
The characterization of mitochondrial permeability transition in clonal pancreatic beta-cells. Multiple modes and regulation.
Authors:Koshkin V, Bikopoulos G, Chan CB, Wheeler MB
Journal:J Biol Chem
PubMed ID:15231823
Mitochondrial permeability transition (MPT), which contributes substantially to the regulation of normal mitochondrial metabolism, also plays a crucial role in the initiation of cell death. It is known that MPT is regulated in a tissue-specific manner. The importance of MPT in the pancreatic beta-cell is heightened by the fact that ... More
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