Vitronectin (VTN-N) Recombinant Human Protein, Truncated
Vitronectin (VTN-N) Recombinant Human Protein, Truncated
Citas y referencias (1)
Gibco™

Vitronectin (VTN-N) Recombinant Human Protein, Truncated

Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs).
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Número de catálogoCantidad
A3180410 mL = 5 mg
A147001 mL = 0,5 mg
Número de catálogo A31804
Precio (CLP)
831.684
Each
Añadir al carro de la compra
Cantidad:
10 mL = 5 mg
Precio (CLP)
831.684
Each
Añadir al carro de la compra

Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8 Medium (Cat. No. A1517001), VTN-N has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines.

  • Proven to maintain pluripotency and normal growth characteristics in multiple PSC lines when used with Essential 8 Medium
  • Supports PSC growth for >50 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages
  • Supports human pluripotent stem cell attachment and survival better than wild-type vitronectin when used in conjunction with Essential 8 Medium
  • Optimized—designed in the laboratory of James Thomson for use with Essential 8 Medium
  • Defined—for reduced variability in your PSC cultures
  • Supports human pluripotent stem cell attachment and survival better than wild type vitronectin
  • Optimal environment for feeder-free pluripotent stem cell culture
  • Defined, recombinant human protein that reduces variability in your PSC cultures compared to human-plasma derived vitronectin and standard basement membrane extracts (BMEs)
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Concentración500 μg/mL
Sistema de expresiónE. coli
Forma de proteínaRecombinant
Subtipo de proteínaVitronectin
Grado de pureza o calidadCell Culture Grade, Endotoxin-Screened
Cantidad10 mL = 5 mg
Categoría de investigaciónStem Cell Research
Condiciones de envíoDry Ice
Volumen (métrico)10 mL
Para utilizar con (aplicación)Stem Cell Culture
FormularioLiquid
RecombinanteRecombinant
EspecieHuman
Unit SizeEach
Contenido y almacenamiento
Store at -80°C and protect from light.

Preguntas frecuentes

What substrate should I use with the PSC Definitive Endoderm Induction Kit?

We recommend using Vitronectin (VTN-N) Recombinant Human Protein, Truncated (Cat. No. A14700), but Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301 or Cat. No. A1413302) can also be used.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can pluripotent stem cells (PSCs) cryopreserved in a different culture condition be thawed and subsequently cultured in Gibco Essential 8 Medium and on vitronectin (VTN-N)?

Yes. PSCs cryopreserved from cultures of mTeSR Medium and BD Matrigel Basement Membrane Matrix may be thawed into Gibco Essential 8 Medium and plated on VTN-N. Certain lines may benefit from thawing into the medium and substrate they were growing in at the time of cryopreservation. Then at the next passage, use EDTA to passage the cells into Gibco Essential 8 Medium and VTN-N.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use enzymes such as dispase and collagenase for passaging cells cultured in Gibco Essential 8 Medium and on VTN-N?

Enzymes such as dispase and collagenase do not work well with cells cultured in Gibco Essential 8 Medium on VTN-N. Use of these enzymes for passaging cells results in compromised viability and attachment.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the recommended passaging method to use with Gibco Essential 8 Medium and on Gibco Vitronectin (VTN-N)?

Cells cultured in Gibco Essential 8 Medium and VTN-N need to be passaged with EDTA.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What will my cells look like growing in Gibco Essential 8 Medium and on Gibco Vitronectin (VTN-N)?

You should expect to see normal pluripotent stem cell (PSC) morphology. The expected morphology of PSCs is demonstrated specifically by tightly packed colonies with defined borders and a high nucleus-to-cytoplasm ratio.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Vitronectin (VTN-N) Recombinant Human Protein, Truncated FAQs

Citations & References (1)

Citations & References
Abstract
A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids.
Authors:Yang L, Han Y, Nilsson-Payant BE, Gupta V, Wang P, Duan X, Tang X, Zhu J, Zhao Z, Jaffré F, Zhang T, Kim TW, Harschnitz O, Redmond D, Houghton S, Liu C, Naji A, Ciceri G, Guttikonda S, Bram Y, Nguyen DT, Cioffi M, Chandar V, Hoagland DA, Huang Y, Xiang J, Wang H, Lyden D, Borczuk A, Chen HJ, Studer L, Pan FC, Ho DD, tenOever BR, Evans T, Schwartz RE, Chen S
Journal:Cell Stem Cell
PubMed ID:32579880
SARS-CoV-2 has caused the COVID-19 pandemic. There is an urgent need for physiological models to study SARS-CoV-2 infection using human disease-relevant cells. COVID-19 pathophysiology includes respiratory failure but involves other organ systems including gut, liver, heart, and pancreas. We present an experimental platform comprised of cell and organoid derivatives from ... More