Alexa Fluor™ 555-aha-dUTP, 1 mM in TE buffer
Alexa Fluor™ 555-aha-dUTP, 1 mM in TE buffer
Citas y referencias (5)
Invitrogen™

Alexa Fluor™ 555-aha-dUTP, 1 mM in TE buffer

Los nucleótidos 5-aminohexilacrilamido-dUTP (aha-dUTP) etiquetados se modifican en la posición C-5 de la uridina con un ligador de hexilacrilamida único,Más información
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Número de catálogoCantidad
A3276250 μl
Número de catálogo A32762
Precio (CLP)
1.015.555
Each
Añadir al carro de la compra
Cantidad:
50 μl
Precio (CLP)
1.015.555
Each
Añadir al carro de la compra
Los nucleótidos 5-aminohexilacrilamido-dUTP (aha-dUTP) etiquetados se modifican en la posición C-5 de la uridina con un ligador de hexilacrilamida único, que sirve como espaciador entre el nucleótido y el colorante. Este espaciador reduce las interacciones entre el nucleótido y el tinte, lo que da como resultado conjugados más brillantes y mayor accesibilidad al hapteno para los reactivos de detección secundarios. Los nucleótidos marcados pueden usarse para generar sondas de hibridación de ácidos nucleicos etiquetados para muchas aplicaciones de biología molecular y citogenética molecular, incluyendo técnicas multicolores.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración1 mM
Para utilizar con (aplicación)Biología molecular
Etiqueta o tinteColorante Alexa Fluor
ModificaciónAHA (5-aminohexilacrilamido)
Línea de productosAlexa Fluor
Tipo de productoNucleótido etiquetado
Cantidad50 μl
Condiciones de envíoHielo húmedo
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.

Preguntas frecuentes

Can dU-containing DNA produced by amplification with dUTP be cut by restriction enzymes?

You will need to confirm with the manufacturer of the restriction enzyme you are using to find out whether or not it can recognize dUTP-containing DNA.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Citations & References (5)

Citations & References
Abstract
Transcriptome for photobiological hydrogen production induced by sulfur deprivation in the green alga Chlamydomonas reinhardtii.
Authors:Nguyen AV, Thomas-Hall SR, Malnoë A, Timmins M, Mussgnug JH, Rupprecht J, Kruse O, Hankamer B, Schenk PM,
Journal:Eukaryot Cell
PubMed ID:18708561
'Photobiological hydrogen production using microalgae is being developed into a promising clean fuel stream for the future. In this study, microarray analyses were used to obtain global expression profiles of mRNA abundance in the green alga Chlamydomonas reinhardtii at different time points before the onset and during the course of ... More
Effects of nitroglycerin or pentaerithrityl tetranitrate treatment on the gene expression in rat hearts: evidence for cardiotoxic and cardioprotective effects.
Authors:Pautz A, Rauschkolb P, Schmidt N, Art J, Oelze M, Wenzel P, Förstermann U, Daiber A, Kleinert H,
Journal:Physiol Genomics
PubMed ID:19417013
Nitroglycerin (NTG) and pentaerithrityl tetranitrate (PETN) are organic nitrates used in the treatment of angina pectoris, myocardial infarction, and congestive heart failure. Recent data show marked differences in the effects of NTG and PETN on the generation of reactive oxygen species. These differences are attributed to different effects of NTG ... More
Transcriptomic signatures of alterations in a myoblast cell line infected with four distinct strains of Trypanosoma cruzi.
Authors:Adesse D, Iacobas DA, Iacobas S, Garzoni LR, Meirelles Mde N, Tanowitz HB, Spray DC,
Journal:Am J Trop Med Hyg
PubMed ID:20439965
We examined the extent to which different Trypanosoma cruzi strains induce transcriptomic changes in cultured L(6)E(9) myoblasts 72 hours after infection with Brazil (TC I), Y (TC II), CL (TC II), and Tulahuen (TC II) strains. Expression of 6,289 distinct, fully annotated unigenes was quantified with 27,000 rat oligonucleotide arrays ... More
Abnormal distribution of inositol 1,4,5-trisphosphate receptors in human muscle can be related to altered calcium signals and gene expression in Duchenne dystrophy-derived cells.
Authors:Cárdenas C, Juretic N, Bevilacqua JA, García IE, Figueroa R, Hartley R, Taratuto AL, Gejman R, Riveros N, Molgó J, Jaimovich E,
Journal:FASEB J
PubMed ID:20395455
Inositol 1,4,5-trisphosphate (IP(3)) receptors (IP(3)Rs) drive calcium signals involved in skeletal muscle excitation-transcription coupling and plasticity; IP(3)R subtype distribution and downstream events evoked by their activation have not been studied in human muscle nor has their possible alteration in Duchenne muscular dystrophy (DMD). We studied the expression and localization of ... More
A novel multiple FISH array for the detection of genetic aberrations in cancer.
Authors:Li R, Liu Z, Fan T, Jiang F
Journal:Lab Invest
PubMed ID:16518404
Interphase multicolor fluorescence in situ hybridization (IM-FISH) has great promise for improving cancer diagnosis because it can directly visualize multiple changes in chromosomes and gene copy number on a cell-to-cell basis. However, no more than four targets can be detected simultaneously by current commercially available IM-FISH protocols, and the DNA ... More