Genexus™ Barcodes 33-64 AS

The Ion Torrent Genexus Barcodes 33-64 AS kit provides one plate filled with 32 dried-down, single-use barcodes and covered withMás información

Número de catálogo	Cantidad
A40259	32 Reactions

Número de catálogo A40259

Precio (CLP)

Cantidad:

32 Reactions

The Ion Torrent Genexus Barcodes 33-64 AS kit provides one plate filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted.When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, this kit supports flexible, fully automated preparation and normalization of up to 32 Ion AmpliSeq libraries. Choose from other available barcode kits: Ion Torrent Genexus Barcodes 1-32 AS, 65-96 AS, and 1-96 AS. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 33-64 AS kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Para utilizar con (aplicación)Secuenciación

Para utilizar con (equipo)Genexus™ Integrated Sequencer

BibliotecasTargeted Sequencing Library

N.º de reacciones32

Línea de productosGenexus

Tipo de productoBarcode

Cantidad32 Reactions

Tipo de muestraDNA, RNA

Condiciones de envíoTemperatura ambiente

Paso del flujo de trabajoLibrary Generation

Sequencing TypeGenome, DNA, and RNA Sequencing

Unit SizeEach

Contenido y almacenamiento

Almacenar a temperatura ambiente.

Preguntas frecuentes

What is the level of detection for somatic variants with the Oncomine Myeloid Research Assay GX?

Detection of somatic variants with the Oncomine Myeloid Research Assay GX has been verified down to 5% allele frequency.

Which extraction kits are compatible with Oncomine Myeloid Research Assay GX?

Here is a list of kits that are compatible with Oncomine Myeloid Research Assay GX:

Nucleic acid isolation - RNA samples
- MagMAX mirVana Total RNA Isolation Kit, Cat. No. A27828
- PureLink Total RNA Blood Kit, Cat. No. K156001
- RNaseZap RNase Decontamination Solution, Cat. No. AM9780, AM9782, AM9784

Nucleic acid isolation - DNA samples
- MagMAX DNA Multi-Sample Ultra 2.0 Kit, Cat. No. A36570
- PureLink Genomic DNA Mini Kit, Cat. No. K1820-01

What are the sample input requirements for Oncomine Myeloid Research Assay GX?

The recommended minimum sample input is 27.75 ng of DNA (1.11 ng/µL minimum concentration) and 14.25 ng of RNA (0.95 ng/µL minimum concentration).

What sample controls should I use with the Oncomine Myeloid Research Assay GX?

The recommended controls are:

DNA Controls:
- Seraseq® Myeloid Mutation DNA Mix, Cat. No. 0710-0408 (SeraCare®)
- Myeloid DNA Reference Standard, Cat. No. HD829 (Horizon™)
- AcroMetrix™ Oncology Hotspot Control, Cat. No. 969056

RNA Controls:
- Universal Human Reference RNA, Cat. No. 740000 (Agilent™)
- Seraseq® Myeloid Fusion RNA Mix, Cat. No. 0710-0407 (SeraCare®)

How many Oncomine Myeloid Research Assay GX samples can be multiplexed on a GX5 Chip?

Multiplex sequencing of up to 8 Oncomine Myeloid Research Assay GX samples (8 DNA+ 8 RNA) per lane on a GX5 Chip can be performed in a single run. There are 4 lanes on the GX5 Chip, so 32 total samples (32 DNA+ 32 RNA) can be run on one GX5 Chip (8 samples per run).

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