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Citas y referencias (53)
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4-acetamido-4'-maleimidstilbeno-2,2'-ácido disulfónico, sal de disodio
Nuestro 4-acetamido-4'-maleimidstilbeno-2,2'- ácido disulfónico es un reactivo al tiol soluble en agua con alta polaridad e impermeabilidad de la membrana.Más información
| Número de catálogo | Cantidad |
|---|---|
| A485 | 25 mg |
Número de catálogo A485
Precio (CLP)
461.617
Each
Cantidad:
25 mg
Precio (CLP)
461.617
Each
Nuestro 4-acetamido-4'-maleimidstilbeno-2,2'- ácido disulfónico es un reactivo al tiol soluble en agua con alta polaridad e impermeabilidad de la membrana. Este colorante polisulfonado es útil para determinar si las proteínas que contienen tiol y las cadenas de polipéptidos están expuestas en la superficie de la membrana extracelular o citoplasmática.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Reactividad químicaTiol
Etiqueta o tinteStilbeno
Tipo de producto4-acetamido-4'-maleimidstilbeno-2,2'-ácido disulfónico
Cantidad25 mg
Fracción reactivaMaleimida
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaColorantes clásicos
Unit SizeEach
Contenido y almacenamiento
Almacenar en congelador (de -5 a -30 °C).
Citations & References (53)
Citations & References
Abstract
AppA is a blue light photoreceptor that antirepresses photosynthesis gene expression in Rhodobacter sphaeroides.
Journal:Cell
PubMed ID:12230978
Photosynthetic bacteria regulate photosystem synthesis in response to alterations in oxygen tension and light intensity. In this study we show that the PpsR repressor from Rhodobacter sphaeroides binds to DNA in a redox-dependent manner through the formation/breakage of an intramolecular disulfide bond. We also demonstrate that PpsR is antagonized by
Protein-protein interactions between UDP-glucuronosyltransferase isozymes in rat hepatic microsomes.
Journal:Biochemistry
PubMed ID:9188715
'The interactions between UDP-glucuronosyltransferase (UGT) isozymes, UGT1s and UGT2B1, in rat hepatic microsomes were investigated using an immunopurification technique with anti-peptide antibodies and a chemical cross-linking strategy. A 50 kDa protein coimmunopurified with UGT1s was identified as UGT2B1 by amino-terminal sequencing and immunodetection with anti-peptide antibody against UGT2B1. Evidence for
Extracellular DsbA-insensitive folding of Escherichia coli heat-stable enterotoxin STa in vitro.
Journal:J Biol Chem
PubMed ID:10744753
'To study the folding of human Escherichia coli heat-stable enterotoxin STh, we used the major protein subunit of CS31A fimbriae (ClpG) as a marker of STh secretion and a provider of a signal peptide. We established that STh genetically fused to the N or C terminus of ClpG was able
Investigation of the extracellular accessibility of the connecting loop between membrane domains I and II of the bradykinin B2 receptor.
Journal:J Biol Chem
PubMed ID:10329674
'In analogy to the structure of rhodopsin, the seven hydrophobic segments of G-protein-coupled receptors are supposed to form seven membrane-spanning alpha-helices. To analyze the topology of the bradykinin B2 receptor, we raised site-directed antibodies to peptides corresponding to the loop regions and the amino and carboxyl terminus of this receptor.
Cysteine-scanning mutagenesis reveals a highly amphipathic, pore-lining membrane-spanning helix in the glutamate transporter GltT.
Journal:J Biol Chem
PubMed ID:11148213
'The carboxyl-terminal membrane-spanning segment 8 of the glutamate transporter GltT of Bacillus stearothermophilus was studied by cysteine-scanning mutagenesis. 21 single cysteine mutants were constructed in a stretch ranging from Gly-374 to Gln-404. Two mutants were not expressed, four were inactive, and two showed severely reduced glutamate transport activity. Cysteine mutations