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Citas y referencias (16)
Invitrogen™
BODIPY™ FL Maleimide (BODIPY™ FL N-(2-Aminoethyl))Maleimide)
La maleimida BODIPY™ FL reactiva al tiol produce conjugados de colorante electrónicamente neutros que son espectralmente similares al colorante deMás información
| Número de catálogo | Cantidad |
|---|---|
| B10250 | 5 mg |
Número de catálogo B10250
Precio (CLP)
458.181
Each
Cantidad:
5 mg
Precio (CLP)
458.181
Each
La maleimida BODIPY™ FL reactiva al tiol produce conjugados de colorante electrónicamente neutros que son espectralmente similares al colorante de fluoresceína con carga negativa. La falta de carga iónica de este colorante produce efectos mínimos en los puntos isoeléctricos de las proteínas estándar conjugadas con este fluoróforo. El colorante BODIPY™ FL de tamaño pequeño y vida útil de estado de excitación relativamente larga ha demostrado ser útil para estudiar la interacción ligando-receptor por polarización de fluoresencia. Además, el colorante BODIPY™ FL tiene poca o ninguna superposición espectral con colorantes de longitud de onda más larga como el colorante Texas Red™ y la tetrametilrodamina, lo que lo convierte en un fluoróforo verde útil para aplicaciones multicolores.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Reactividad químicaTiol
Etiqueta o tinteBODIPY™ FL
Tipo de productoMaleimida
Cantidad5 mg
Fracción reactivaMaleimida
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaColorantes BODIPY
Línea de productosBODIPY
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Citations & References (16)
Citations & References
Abstract
Small vertical movement of a K+ channel voltage sensor measured with luminescence energy transfer.
Journal:Nature
PubMed ID:16094368
'Voltage-gated ion channels open and close in response to voltage changes across electrically excitable cell membranes. Voltage-gated potassium (Kv) channels are homotetramers with each subunit constructed from six transmembrane segments, S1-S6 (ref. 2). The voltage-sensing domain (segments S1-S4) contains charged arginine residues on S4 that move across the membrane electric
Quantitation of microparticles released from coated-platelets.
Journal:J Thromb Haemost
PubMed ID:16102115
'Dual agonist stimulation of platelets with thrombin and convulxin results in generation of coated-platelets, a sub-population of cells known formerly as COAT-platelets (collagen and thrombin). Coated-platelets retain several procoagulant proteins on their surface and express phosphatidylserine (PS). In this report, we utilize a new methodology to demonstrate that coated-platelets also
Evaluation of disulfide reduction during receptor-mediated endocytosis by using FRET imaging.
Journal:Proc Natl Acad Sci U S A
PubMed ID:16950881
'Despite functional evidence for disulfide bond-reducing activity in endosomal compartments, the mechanistic details pertaining to such process (e.g., kinetics and sites of disulfide reduction) remain largely controversial. To address these questions directly, we have synthesized a previously uncharacterized fluorescent folate conjugate, folate-(BODIPY FL)-SS-rhodamine (folate-FRET), that changes fluorescence from red to
An in vitro fluorescence screen to identify antivirals that disrupt hepatitis B virus capsid assembly.
Journal:Nat Biotechnol
PubMed ID:16474383
'Virus assembly has not been routinely targeted in the development of antiviral drugs, in part because of the lack of tractable methods for screening in vitro. We have developed an in vitro assay of hepatitis B virus (HBV) capsid assembly, based on fluorescence quenching of dye-labeled capsid protein, for testing
The achondroplasia mutation does not alter the dimerization energetics of the fibroblast growth factor receptor 3 transmembrane domain.
Journal:Biochemistry
PubMed ID:16634636
The Gly380 --> Arg mutation in the TM domain of fibroblast growth factor receptor 3 (FGFR3) of the RTK family is linked to achondroplasia, the most common form of human dwarfism. The molecular mechanism of pathology induction is under debate, and two different mechanisms have been proposed to contribute to