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Citas y referencias (17)
Invitrogen™
Fluorescein biotin, 5-((N-(5-(N-(6-(biotinoyl)amino)hexanoyl)amino)pentyl)thioureidyl)fluorescein
La biotina de fluoresceína se desarrolló como alternativa a la biotina radioactiva para detectar y cuantificar los sitios de uniónMás información
| Número de catálogo | Cantidad |
|---|---|
| B1370 | 5 mg |
Número de catálogo B1370
Precio (CLP)
-
Cantidad:
5 mg
La biotina de fluoresceína se desarrolló como alternativa a la biotina radioactiva para detectar y cuantificar los sitios de unión a biotina mediante fluorescencia o absorbancia. Al unirse a la avidina o a la estreptavidina, se apaga la fluorescencia o absorbancia de esta molécula.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Etiqueta o tinteFITC (fluoresceína)
Tipo de productoSondas moleculares de fluoresceína y biotina
Cantidad5 mg
Condiciones de envíoTemperatura ambiente
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.
Citations & References (17)
Citations & References
Abstract
Functional consequences of insertions and deletions in the complementarity-determining regions of human antibodies.
Journal:J Biol Chem
PubMed ID:12237318
'Insertions and deletions of nucleotides in the genes encoding the variable domains of antibodies are natural components of the hypermutation process, which may expand the available repertoire of hypervariable loop lengths and conformations. Although insertion of amino acids has also been utilized in antibody engineering, little is known about the
Continuous-flow, on-line monitoring of biospecific interactions using electrospray mass spectrometry.
Journal:Anal Chem
PubMed ID:11534702
'A continuous-flow analytical screening system is presented using electrospray mass spectrometry to measure the interaction of biologically active compounds with soluble affinity proteins. The biochemical detection system is based on a solution-phase, homogeneous assay. In a first step, compounds to be screened (e.g., biotinylated compounds, concentration range 10-1,000 nmol/L) are
Randomly ordered addressable high-density optical sensor arrays.
Journal:Anal Chem
PubMed ID:9553489
'Array-based sensors provide an architecture for multianalyte sensing. In this paper, we report a new approach for array fabrication. Sensors are made by immobilizing different reactive chemistries on the surfaces of microspheres. Sensor arrays are prepared by randomly distributing a mixture of microsphere sensors on an optical substrate containing thousands
Using affinity capillary electrophoresis to determine binding stoichiometries of protein-ligand interactions.
Journal:Biochemistry
PubMed ID:8075061
'We have developed a new method utilizing affinity capillary electrophoresis (ACE) for the determination of binding stoichiometries in biochemical systems. Using the same concentration of a ligand in the sample and the electrophoresis buffer, the appearance of an inverted peak corresponding to the free ligand in the resulting electropherogram provides
Peptides, antibodies, and FRET on beads in flow cytometry: A model system using fluoresceinated and biotinylated beta-endorphin.
Journal:Cytometry
PubMed ID:10451503
'BACKGROUND: Particulate surfaces such as beads are routinely used as platforms for molecular assembly for fundamental and practical applications in flow cytometry. Molecular assembly is transduced as the direct analysis of fluorescence, or as a result of fluorescence resonance energy transfer. Binding of fluorescent ligands to beads sometimes alters their