Biocytin (ε-Biotinoyl-L-Lysine)
Biocytin (ε-Biotinoyl-L-Lysine)
Citas y referencias (174)
Invitrogen™

Biocytin (ε-Biotinoyl-L-Lysine)

La biocitina se puede utilizar como trazadores anterógrados y transneuronales microinyectables que se pueden fijar con fijadores basados en aldehído.Más información
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Número de catálogoCantidad
B1592100 mg
Número de catálogo B1592
Precio (CLP)
283.659
Each
Añadir al carro de la compra
Cantidad:
100 mg
Precio (CLP)
283.659
Each
Añadir al carro de la compra
La biocitina se puede utilizar como trazadores anterógrados y transneuronales microinyectables que se pueden fijar con fijadores basados en aldehído. Además, la amina primaria de la lisina puede acoplarse de forma reversible a aldehídos y cetonas para formar una base de Schiff, que puede reducirse a un derivado de amina estable generado por el borohidruro de sodio (NaBH4) o cianoborohidruro de sodio (NaCNH3). Los ácidos carboxílicos de las proteínas y otros biopolímeros solubles en agua pueden acoplarse a esta molécula en una solución acuosa usando carbodiimidas solubles en agua como EDAC (E2247).
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de etiquetaBiotina y otros haptenos
Tipo de productoBiocitina
Cantidad100 mg
Tipo de reactivoBiocitinas
Condiciones de envíoTemperatura ambiente
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente.

Citations & References (174)

Citations & References
Abstract
Authors:
Journal:
PubMed ID:7905516
Human serum biotinidase. cDNA cloning, sequence, and characterization.
Authors:Cole H, Reynolds TR, Lockyer JM, Buck GA, Denson T, Spence JE, Hymes J, Wolf B
Journal:J Biol Chem
PubMed ID:7509806
Biotinidase (EC 3.5.1.12) catalyzes the hydrolysis of biocytin, the product of biotin-dependent carboxylase degradation, to biotin and lysine. Biotinidase deficiency is an inherited metabolic disorder of biotin recycling that is characterized by neurological and cutaneous abnormalities, and can be successfully treated with biotin supplementation. Sequences of tryptic peptides of the ... More
Purification of biotinidase from human plasma and its activity on biotinyl peptides.
Authors:Craft DV, Goss NH, Chandramouli N, Wood HG
Journal:Biochemistry
PubMed ID:3925986
Biotinidase catalyzes the hydrolysis of N epsilon-biotinyllysine (biocytin) to form biotin and free lysine. The enzyme has been purified 4800-fold from outdated human plasma and was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to have a molecular weight of (76 +/- 2) X 10(3). The same molecular weight was found ... More
Deformation of network connectivity in the inferior olive of connexin 36-deficient mice is compensated by morphological and electrophysiological changes at the single neuron level.
Authors:De Zeeuw CI, Chorev E, Devor A, Manor Y, Van Der Giessen RS, De Jeu MT, Hoogenraad CC, Bijman J, Ruigrok TJ, French P, Jaarsma D, Kistler WM, Meier C, Petrasch-Parwez E, Dermietzel R, Sohl G, Gueldenagel M, Willecke K, Yarom Y
Journal:J Neurosci
PubMed ID:12805309
Compensatory mechanisms after genetic manipulations have been documented extensively for the nervous system. In many cases, these mechanisms involve genetic regulation at the transcription or expression level of existing isoforms. We report a novel mechanism by which single neurons compensate for changes in network connectivity by retuning their intrinsic electrical ... More
Structure and function of retinal ganglion cells innervating the cat's geniculate wing: an in vitro study.
Authors:Pu M, Berson DM, Pan T
Journal:J Neurosci
PubMed ID:8027783
'We have examined in vitro the morphology and visual response properties of retinal ganglion cells innervating a component of the cat's lateral geniculate nucleus known as the geniculate wing (or retinorecipient zone of the pulvinar). Ganglion cells were first labeled in situ by retrograde transport of fluorescent microspheres from the ... More
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