BacLight™ Green Bacterial Stain
<i>Bac</i>Light&trade; Green Bacterial Stain
Citas y referencias (4)
Invitrogen™

BacLight™ Green Bacterial Stain

Las tinciones bacterianas BacLight Green (n.º de cat. B-35000) y BacLight Red (n.º de cat. B-35001) son reactivos de etiquetadoMás información
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Número de catálogoCantidad
B3500020 x 50 μg
Número de catálogo B35000
Precio (CLP)
474.217
Each
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Cantidad:
20 x 50 μg
Precio (CLP)
474.217
Each
Añadir al carro de la compra
Las tinciones bacterianas BacLight Green (n.º de cat. B-35000) y BacLight Red (n.º de cat. B-35001) son reactivos de etiquetado fluorescente para la detección y el control de bacterias. Estos dos colorantes no son tinciones de ácidos nucleicos. Las bacterias teñidas con tinciones bacterianas BacLight Green y BacLight Red presentan fluorescencia verde y roja brillante (absorción/emisión ∼ 480/516 y ∼ 581/644 nm, respectivamente), y pueden resolverse utilizando los canales citométricos de flujo o filtros de microscopía de fluorescencia adecuados. Las tinciones bacterianas BacLight son compatibles con los métodos de fijación con formaldehído o alcohol.

Consulte información adicional sobre todos los productos de análisis microbiológico.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaBacterias
Método de detecciónFluorescente
Tipo de coloranteOtras etiquetas o colorantes
FormularioSólido
FormatoTubos, portaobjetos
Cantidad20 x 50 μg
Condiciones de envíoTemperatura ambiente
SolubilidadDMSO (dimetilsulfóxido)
Emission480⁄ 561
Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo
Línea de productosBacLight
Tipo de productoTinción bacteriana
Unit SizeEach
Contenido y almacenamiento
Contiene 20 viales de tinción bacteriana BacLight™ Green (50 ug por vial). Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.

Preguntas frecuentes

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What optical filter do your recommend with the BacLight Green Bacterial Stain?

We recommend filters suitable for fluorescein/FITC with the BacLight Green kit.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (4)

Citations & References
Abstract
Identification of broadly protective human antibodies to Pseudomonas aeruginosa exopolysaccharide Psl by phenotypic screening.
Authors:DiGiandomenico A, Warrener P, Hamilton M, Guillard S, Ravn P, Minter R, Camara MM, Venkatraman V, Macgill RS, Lin J, Wang Q, Keller AE, Bonnell JC, Tomich M, Jermutus L, McCarthy MP, Melnick DA, Suzich JA, Stover CK,
Journal:J Exp Med
PubMed ID:22734046
Pseudomonas aeruginosa is a leading cause of hospital-associated infections in the seriously ill, and the primary agent of chronic lung infections in cystic fibrosis patients. A major obstacle to effective control of P. aeruginosa infections is its intrinsic resistance to most antibiotic classes, which results from chromosomally encoded drug-efflux systems ... More
Analysis of adherence, biofilm formation and cytotoxicity suggests a greater virulence potential of Gardnerella vaginalis relative to other bacterial-vaginosis-associated anaerobes.
Authors:Patterson JL, Stull-Lane A, Girerd PH, Jefferson KK,
Journal:Microbiology
PubMed ID:19910411
'Worldwide, bacterial vaginosis (BV) is the most common vaginal disorder in women of childbearing age. BV is characterized by a dramatic shift in the vaginal microflora, involving a relative decrease in lactobacilli, and a proliferation of anaerobes. In most cases of BV, the predominant bacterial species found is Gardnerella vaginalis. ... More
Teleost skin, an ancient mucosal surface that elicits gut-like immune responses.
Authors:Xu Z, Parra D, Gómez D, Salinas I, Zhang YA, von Gersdorff Jørgensen L, Heinecke RD, Buchmann K, LaPatra S, Sunyer JO,
Journal:
PubMed ID:23884653
'Skin homeostasis is critical to preserve animal integrity. Although the skin of most vertebrates is known to contain a skin-associated lymphoid tissue (SALT), very little is known about skin B-cell responses as well as their evolutionary origins. Teleost fish represent the most ancient bony vertebrates containing a SALT. Due to ... More
SadA, a trimeric autotransporter from Salmonella enterica serovar Typhimurium, can promote biofilm formation and provides limited protection against infection.
Authors:Raghunathan D, Wells TJ, Morris FC, Shaw RK, Bobat S, Peters SE, Paterson GK, Jensen KT, Leyton DL, Blair JM, Browning DF, Pravin J, Flores-Langarica A, Hitchcock JR, Moraes CT, Piazza RM, Maskell DJ, Webber MA, May RC, MacLennan CA, Piddock LJ, Cunningham AF, Henderson IR,
Journal:Infect Immun
PubMed ID:21859856
Salmonella enterica is a major cause of morbidity worldwide and mortality in children and immunocompromised individuals in sub-Saharan Africa. Outer membrane proteins of Salmonella are of significance because they are at the interface between the pathogen and the host, they can contribute to adherence, colonization, and virulence, and they are ... More