Kit de preparación de muestras NativePAGE™
Kit de preparación de muestras NativePAGE™
Invitrogen™

Kit de preparación de muestras NativePAGE™

El kit de preparación de muestras NativePAGE™ incluye reactivos de preparación de muestras para la electroforesis en gel nativa. ElMás información
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Número de catálogoCantidad
BN20081 Kit
Número de catálogo BN2008
Precio (CLP)
370.062
Each
Añadir al carro de la compra
Cantidad:
1 Kit
Precio (CLP)
370.062
Each
Añadir al carro de la compra
El kit de preparación de muestras NativePAGE™ incluye reactivos de preparación de muestras para la electroforesis en gel nativa. El kit incluye dos soluciones detergentes listas para usar, DDM (n-dodecil-β-D-maltósido) al 10 % y digitonina al 5 %, que mejoran la solubilidad de las proteínas hidrófobas y de membrana durante la preparación de muestras.

Las muestras preparadas con un 10 % de DDM y 5 % de digitonina, o el kit de preparación de muestras NativePAGE™ son compatibles con los geles Bis-Tris NativePAGE™ Novex para la electroforesis nativa y muestran una mayor resolución y menos estrías.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
TampónTampones de carga de muestras
Compatibilidad del gelGeles NativePAGE™
Cantidad1 Kit
Condiciones de envíoHielo húmedo
Tipo de gelGel Native
Línea de productosNativePAGE
Tipo de productoKit de preparación de muestras PAGE
Unit SizeEach
Contenido y almacenamiento
Contiene:
• 1 ml de DDM al 10 %
• 1 ml de digitonina al 5 %
• 10 ml de tampón de muestra NativePAGE™ 4X
• 0,5 ml de aditivo para muestras NativePAGE™ 5 % G-250.

Almacenar el kit de 2 °C a 8 °C.

Preguntas frecuentes

I would like to run a NativePAGE gel. Which of your protein standards should I use?

We recommend using the NativeMark Unstained Protein Standard, Cat. No. LC0725 for native gel electrophoresis with Tris-Glycine, NuPAGE Tris-Acetate or NativePAGE gels.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use the NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels?

We do not recommend using NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels. NuPAGE Invitrogen Bis-Tris gels are optimized for denaturing conditions and have an extremely low operating pH (pH 7.0), which makes it difficult for most proteins to migrate through them in a native state.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the NativePAGE Sample and Running buffers with Invitrogen Tris-Glycine gels or NuPAGE Tris-Acetate gels for native applications?

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate or Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the NativePAGE Sample and Running buffers with Invitrogen Tris-Glycine gels for native applications?

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the NativePAGE Sample and Running buffers with NuPAGE Tris-Acetate gels for native applications?

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate gels. When running Tris-Acetate gels under native conditions, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.