Human Umbilical Vein Endothelial Cells (HUVEC)
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Citas y referencias (2)
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Human Umbilical Vein Endothelial Cells (HUVEC)

Las células primarias endoteliales de vena de cordón umbilical humano (HUVEC) se aíslan de la vena del cordón umbilical humanoMás información
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Número de catálogoCantidad
C0035C1 vial
Número de catálogo C0035C
Precio (CLP)
-
Cantidad:
1 vial
Las células primarias endoteliales de vena de cordón umbilical humano (HUVEC) se aíslan de la vena del cordón umbilical humano y se crioconservan al final del cultivo primario.

Las células:
• Se han comprobado en cuanto a rendimiento: al menos 16 duplicaciones de población cuando se cultiva en el medio mediano 200 complementado con el suplemento endotelial para recipientes grandes (LVES)
• Se garantiza que sean ≥70 % viables
• Se han comprobado en cuanto a micoplasma, bacterias, levaduras u otros hongos, hepatitis B, hepatitis C y virus VIH-1

Cada vial contiene más de 0,5 x 106 células viables que se han crioconservado al final de la fase de cultivo primario en un medio que contiene DMSO al 10 %.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Fuente de dadoresDonante único
N.° de celdas≥500000
Línea de productosCascade Biologics
Tipo de productoCélula endotelial de la vena umbilical humana
Cantidad1 vial
Probado paraHepatitis B (VHB), Hepatitis C (VHC), pruebas de esterilidad (hongos y bacterias), micoplasma, ensayo de rendimiento del crecimiento, VIH-1
AgeRecién nacido (14 días o menos)
Tipo de célulaCélulas endoteliales (vena del cordón umbilical)
Culture TypeCultivo celular adherente
FormularioCrioconservado
EspecieHumano
Unit SizeEach
Contenido y almacenamiento
Almacenar en nitrógeno líquido.

Preguntas frecuentes

What types of large vessel endothelial cells do you offer?

We carry primary human umbilical vein endothelial cells (HUVEC) from one donor or pooled from multiple donors.

What media do you use during the first passage of primary cells?

We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).

How is the population doubling calculated for primary cells?

Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.

What are typical primary cell yields from tissue?

It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.

Are primary cells isolated from tissue using enzymatic digestion?

Yes, our primary cells are isolated from tissue using enzymatic digestion.

View all Human Umbilical Vein Endothelial Cells (HUVEC) FAQs

Citations & References (2)

Citations & References
Abstract
Generation of iPSC line epiHUVEC from human umbilical vein endothelial cells.
Authors:Matz P, Adjaye J,
Journal:
PubMed ID:26987922
Human umbilical vein endothelial cells (HUVECs) were used to generate the iPSC line epiHUVEC employing a combination of three episomal-based plasmids expressing OCT4, SOX2, NANOG, LIN28, c-MYC and KLF4. Pluripotency was confirmed both in vivo and in vitro. The transcriptome profile of epiHUVEC and the human embryonic stem cell line ... More
Angiogenic properties of dehydrated human amnion/chorion allografts: therapeutic potential for soft tissue repair and regeneration.
Authors:Koob TJ, Lim JJ, Massee M, Zabek N, Rennert R, Gurtner G, Li WW,
Journal:
PubMed ID:24817999
Chronic wounds are associated with a number of deficiencies in critical wound healing processes, including growth factor signaling and neovascularization. Human-derived placental tissues are rich in regenerative cytokines and have been shown in randomized clinical trials to be effective for healing chronic wounds. In this study, PURION® Processed (MiMedx Group, ... More