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Ensayo de proliferación celular CyQUANT™ NF
Citas y referencias (7)
Invitrogen™

Ensayo de proliferación celular CyQUANT™ NF

El kit de ensayo de proliferación celular CyQUANT™ NF proporciona un método rápido y sensible para el recuento de célulasMás información
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Número de catálogoTipo de célulaCantidad
C35011Direct Cell10 Microplates
C35013Direct Red Cell10 Microplacas
C35012Direct Cell100 Microplates
C35007NF Cell200 Assays
C7026For cells in culture1000 ensayos
C35006NF Cell1000 Assays
C7027Cell Lysis Buffer50 mL
Número de catálogo C35011
Precio (CLP)
652.014
Each
Tipo de célula:
Direct Cell
Cantidad:
10 Microplates
Precio (CLP)
652.014
Each
El kit de ensayo de proliferación celular CyQUANT™ NF proporciona un método rápido y sensible para el recuento de células en una población y la medición de la proliferación en formato de microplaca.

Características del ensayo de proliferación celular CyQUANT™ NF:

• Más sensible que los ensayos MTT o alamarBlue™
• Rango de detección lineal de 100 a 20.000 células por pocillo (microplaca de 96 pocillos)
• Permite completar los ensayos en una hora

Un método rápido y sencillo para medir la proliferación celular
El ensayo de proliferación celular CyQUANT™ NF no requiere lisis celular, incubaciones largas, radioactividad ni eliminación de tinte de las células. El ensayo CyQUANT™ NF elimina el paso de lisis celular con congelación-descongelación del ensayo de proliferación celular CyQUANT™ original utilizando un tinte fijador de ADN y permeable en célula con un reactivo de permeabilización de la membrana de plasma. El ensayo de proliferación celular CyQUANT™ NF se puede utilizar en cualquiera de los formatos de microplacas de 96 pocillos o 384 pocillos, y está disponible en dos configuraciones: un kit de ensayo 200 (C35007) para pequeños tamaños de muestra y un kit de ensayo 1000 (C35006) para aplicaciones de alto rendimiento.

alamarBlue™ es una marca registrada de TREK Diagnostic Systems.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaDirect Cell
Entorno de cultivoCultivo celular en suspensión, cultivo celular adherente
Método de detecciónFluorescente
Tipo de coloranteCyQuant
Longitud de onda de emisión535 nm
Excitación/emisión508/527 nm
Longitud de onda de excitación480 nm
Formato96-well Plate, 384-well Plate, 1536-well Plate
Tiempo de incubación60 min
Cantidad10 Microplates
Condiciones de envíoTemperatura ambiente
ColorVerde
Para utilizar con (aplicación)Cell Proliferation
Para utilizar con (equipo)Lector de microplacas, Espectrofotómetro, Microscopio
Línea de productosCyQUANT, Molecular Probes
Tipo de productoEnsayo de proliferación celular
Unit SizeEach
Contenido y almacenamiento
  • Tinción directa de ácidos nucleicos de 0,5 ml CyQUANT™ (componente A)
  • Supresor de fondo directo de 2,5 ml CyQUANT™ (componente B)
  • Almacenar entre 2 °C y 25 °C. Desecar y proteger de la luz.

Preguntas frecuentes

MTT cell proliferation plate assays require cellular metabolism to modify the reagent and thus, only live cells will be counted. Is this true for CyQUANT Direct Cell Proliferation Assay, too?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye in it is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a non-cell-permeable quenching reagent in the kit which will both quench extracellular fluorescence (and thus this is a no-wash assay) AND will quench the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

MTT cell proliferation assays require cellular metabolism to turn over the reagent, and thus only live cells will be counted. Is this true for CyQUANT Direct as well?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a cell impermeant quenching reagent in the kit which will quench both extracellular fluorescence (and thus this is a no-wash assay) and the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What volumes should I use to make a 10X stock solution for the CyQUANT Direct Cell Proliferation Assay (Cat. No. C35011, C35012)?

To make a 10X stock solution for the CyQUANT Direct Cell Proliferation Assay (Cat. No. C35011, C35012), we suggest using the following volumes:

1.1 mL         PBS
25 µL         CyQUANT Direct nucleic acid stain (Component A)
125 µL         CyQUANT Direct background suppressor I (Component B)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the components of the CyQUANT Direct Red Cell Proliferation Assay and CyQuant Direct Cell Proliferation Assay kits interchangeable?

No. The components of the CyQUANT Direct Red Cell Proliferation Assay and CyQuant Direct Cell Proliferation Assay kits are not interchangeable.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between the CyQUANT Direct Red Cell Proliferation Assay (Cat. Nos. C35013, C35014) and the green fluorescent CyQuant Direct Cell Proliferation Assay (Cat Nos. C35011, C35012)?

The two kits utilize different dyes and background suppressors, but they function in the same way and provide similar results as can be seen from Figures 1 and 2 in the CyQUANT Direct Red Cell Proliferation Assay product sheet (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018644_CyQUANT_direct_red_assay_PI.pdf). Dyes from both kits are nucleic acid stains and the background suppressors are cell impermeant reagents that quench extracellular fluorescence. They are usable within the same range (100 to 20,000 cells/well in a 96-well format) and in the same workflow.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Ensayo de proliferación celular CyQUANT™ NF FAQs

Citations & References (7)

Citations & References
Abstract
Application of a high-content multiparameter cytotoxicity assay to prioritize compounds based on toxicity potential in humans.
Authors:Abraham VC, Towne DL, Waring JF, Warrior U, Burns DJ,
Journal:J Biomol Screen
PubMed ID:18566484
'Prioritization of compounds based on human hepatotoxicity potential is currently a key unmet need in drug discovery, as it can become a major problem for several lead compounds in later stages of the drug discovery pipeline. The authors report the validation and implementation of a high-content multiparametric cytotoxicity assay based ... More
Activator of G protein signaling 3 promotes epithelial cell proliferation in PKD.
Authors:Nadella R, Blumer JB, Jia G, Kwon M, Akbulut T, Qian F, Sedlic F, Wakatsuki T, Sweeney WE, Wilson PD, Lanier SM, Park F,
Journal:J Am Soc Nephrol
PubMed ID:20488951
'The activation of heterotrimeric G protein signaling is a key feature in the pathophysiology of polycystic kidney diseases (PKD). In this study, we report abnormal overexpression of activator of G protein signaling 3 (AGS3), a receptor-independent regulator of heterotrimeric G proteins, in rodents and humans with both autosomal recessive and ... More
The matricellular protein cysteine-rich protein 61 (CCN1/Cyr61) enhances physiological adaptation of retinal vessels and reduces pathological neovascularization associated with ischemic retinopathy.
Authors:Hasan A, Pokeza N, Shaw L, Lee HS, Lazzaro D, Chintala H, Rosenbaum D, Grant MB, Chaqour B,
Journal:J Biol Chem
PubMed ID:21212276
'Retinal vascular damages are the cardinal hallmarks of retinopathy of prematurity (ROP), a leading cause of vision impairment and blindness in childhood. Both angiogenesis and vasculogenesis are disrupted in the hyperoxia-induced vaso-obliteration phase, and recapitulated, although aberrantly, in the subsequent ischemia-induced neovessel formation phase of ROP. Yet, whereas the histopathological ... More
Selective blockade of cytoskeletal actin remodeling reduces experimental choroidal neovascularization.
Authors:Caballero S, Yang R, Grant MB, Chaqour B,
Journal:Invest Ophthalmol Vis Sci
PubMed ID:21178140
'The efficacy of the peptide Ac-EEED on reducing cell adhesion and proliferation in vitro and choroidal neovascularization (CNV) in vivo was examined. The peptide chimera containing the Ac-EEED sequence was chemically linked to the N terminus of the XMTM delivery peptide from the E(rns) viral surface protein. Ac-EEED or scrambled ... More
Loss of activator of G-protein signaling 3 impairs renal tubular regeneration following acute kidney injury in rodents.
Authors:Regner KR, Nozu K, Lanier SM, Blumer JB, Avner ED, Sweeney WE, Park F,
Journal:FASEB J
PubMed ID:21343176
The intracellular mechanisms underlying renal tubular epithelial cell proliferation and tubular repair following ischemia-reperfusion injury (IRI) remain poorly understood. In this report, we demonstrate that activator of G-protein signaling 3 (AGS3), an unconventional receptor-independent regulator of heterotrimeric G-protein function, influences renal tubular regeneration following IRI. In rat kidneys exposed to ... More
View all Ensayo de proliferación celular CyQUANT™ NF citations