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Citas y referencias (8)
Invitrogen™
5-TAMRA (5-Carboxytetramethylrhodamine), single isomer
La tetrametilrodamina (TMR, TRITC) ha sido un fluoróforo ampliamente utilizado para preparar bioconjugados, especialmente anticuerpos fluorescentes y derivados de avidinaMás información
| Número de catálogo | Cantidad |
|---|---|
| C6121 | 10 mg |
Número de catálogo C6121
Precio (CLP)
400.908
Each
Cantidad:
10 mg
Precio (CLP)
400.908
Each
La tetrametilrodamina (TMR, TRITC) ha sido un fluoróforo ampliamente utilizado para preparar bioconjugados, especialmente anticuerpos fluorescentes y derivados de avidina utilizados en inmunoquímica. Bajo el nombre de TAMRA, el ácido carboxílico de 5-TAMRA también ha alcanzado prominencia como colorante para el etiquetado de oligonucleótidos y aplicaciones automatizadas de secuenciación de ADN.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Reactividad químicaAmina
Etiqueta o tinteIsómeros TAMRA™, TMR (tetrametilrodamina)
Tipo de producto5-TAMRA
Cantidad10 mg
Fracción reactivaÁcido carboxílico
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaColorantes clásicos
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.
Citations & References (8)
Citations & References
Abstract
Extending the applicability of carboxyfluorescein in solid-phase synthesis.
Journal:Bioconjug Chem
PubMed ID:12757391
'Optimized coupling protocols are presented for the efficient and automated generation of carboxyfluorescein-labeled peptides. Side products, generated when applying earlier protocols for the in situ activation of carboxyfluorescein, were eliminated by a simple procedure, yielding highly pure fluorescent peptides and minimizing postsynthesis workup. For the cost-efficient labeling of large compound
Pathway for polyarginine entry into mammalian cells.
Journal:Biochemistry
PubMed ID:14992581
Cationic peptides known as protein transduction domains (PTDs) provide a means to deliver molecules into mammalian cells. Here, nonaarginine (R(9)), the most efficacious of known PTDs, is used to elucidate the pathway for PTD internalization. Although R(9) is found in the cytosol as well as the nucleolus when cells are
Insertion and organization within membranes of the delta-endotoxin pore-forming domain, helix 4-loop-helix 5, and inhibition of its activity by a mutant helix 4 peptide.
Journal:J Biol Chem
PubMed ID:10811807
The pore-forming domain of Bacillus thuringiensis Cry1Ac insecticidal protein comprises of a seven alpha-helix bundle (alpha1-alpha7). According to the "umbrella model," alpha4 and alpha5 helices form a hairpin structure thought to be inserted into the membrane upon binding. Here, we have synthesized and characterized the hairpin domain, alpha4-loop-alpha5, its alpha4
A 'puff and advance' technique for visually controlled staining of turtle retinal ganglion cells.
Journal:J Neurosci Methods
PubMed ID:1696675
We describe a 'puff and advance' technique for visually controlled staining of retinal ganglion cells (GCs) in the unfixed, living retina for light and electron microscopy. Glass microelectrodes are filled with rhodamine-isothiocyanate labeled horseradish peroxidase (Rh-HRP), or Lucifer yellow (LY), or a mixture of both, or with 5,6-carboxytetramethylrhodamine (5,6-Rh) and
High-resolution liquid chromatography of fluorescent dye-labeled nucleic acids.
Journal:Anal Biochem
PubMed ID:7695100
Using 100 mM of triethylammonium acetate as ion-pairing reagent, phosphodiester oligonucleotides labeled fluorescently at their 5' terminus could be separated successfully on alkylated nonporous 2.3-microns poly(styrene-divinylbenzene) particles by means of high-resolution liquid chromatography. Applying excitation wavelengths of 490, 520, 550, and 575 nm, respectively, optimum sensitivity was achieved for the