DRAQ7™ Dye

Name: DRAQ7™ Dye
SKU: D15106

La tinción DRAQ7 es un tinte fluorescente de ADN brillante y fácil de usar que se utiliza para excluir célulasMás información

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Número de catálogo	Cantidad
D15106	200 ensayos
D15105	50 ensayos

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Número de catálogo D15106

Precio (CLP)

Cantidad:

200 ensayos

La tinción DRAQ7 es un tinte fluorescente de ADN brillante y fácil de usar que se utiliza para excluir células no viables por citometría de flujo. Este tinte tiene una excitación máxima de 599/644 nm y una emisión máxima de 678/697 nm.

• Estable a temperatura ambiente
• Tinción rápida de células muertas sin pasos de lavado
• Sin excitación UV ni superposición espectral de emisión con PE

La tinción DRAQ7 es un tinte impermeable a la membrana que tiñe rápidamente el ADN bicatenario (ADNdc) de células muertas o permeables. Las longitudes de onda de 488 a 647 nm pueden excitar este tinte, aunque se excita óptimamente con líneas láser rojas. Su emisión máxima es de 697 nm cuando está intercalada con ADNd.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Cantidad200 ensayos

Tipo de productoTinte

Unit Size200 assays

Contenido y almacenamiento

Almacenar a temperatura ambiente.

Citations & References (4)

Citations & References

Abstract

Real-time cell viability assays using a new anthracycline derivative DRAQ7®.

Authors:Akagi J, Kordon M, Zhao H, Matuszek A, Dobrucki J, Errington R, Smith PJ, Takeda K, Darzynkiewicz Z, Wlodkowic D,

Journal:Cytometry A

PubMed ID:23165976

'The exclusion of charged fluorescent dyes by intact cells has become a well-established assay for determining viability of cells. In search for a noninvasive fluorescent probe capable of long-term monitoring of cell death in real-time, we evaluated a new anthracycline derivative DRAQ7. The novel probe does not penetrate the plasma ... More

BET Bromodomain Inhibitor iBET151 Impedes Human ILC2 Activation and Prevents Experimental Allergic Lung Inflammation.

Authors:Kerscher B, Barlow JL, Rana BM, Jolin HE, Gogoi M, Bartholomew MA, Jhamb D, Pandey A, Tough DF, van Oosterhout AJM, McKenzie ANJ,

Journal:Front Immunol

PubMed ID:31024538

'Group 2 innate lymphoid cells (ILC2) increase in frequency in eczema and allergic asthma patients, and thus represent a new therapeutic target cell for type-2 immune-mediated disease. The bromodomain and extra-terminal (BET) protein family of epigenetic regulators are known to support the expression of cell cycle and pro-inflammatory genes during ... More

Lysosomotropic challenge of mast cells causes intra-granular reactive oxygen species production.

Authors:Paivandy A, Eriksson J, Melo FR, Sellin ME, Pejler G,

Journal:Cell Death Discov

PubMed ID:31123601

'Mast cells contribute to the pathology of allergic and other disorders. Strategies to interfere with harmful mast cell-related activities are therefore warranted. Previously we established a principle for inducing selective apoptosis of mast cells, by the use of lysosomotropic agents that cause secretory granule permeabilization, leading to production of reactive ... More

Cytokine-induced translocation of GRP78 to the plasma membrane triggers a pro-apoptotic feedback loop in pancreatic beta cells.

Authors:Vig S, Buitinga M, Rondas D, Crèvecoeur I, van Zandvoort M, Waelkens E, Eizirik DL, Gysemans C, Baatsen P, Mathieu C, Overbergh L,

Journal:Cell Death Dis

PubMed ID:30952835

The 78-kDa glucose-regulated protein (GRP78) is an ubiquitously expressed endoplasmic reticulum chaperone, with a central role in maintaining protein homeostasis. Recently, an alternative role for GRP78 under stress conditions has been proposed, with stress-induced extracellular secretion and translocation of GRP78 to the cell surface where it acts as a multifunctional ... More