Cuando el laurdan de la sonda lipofílica se incorpora a las membranas, su espectro de fluorescencia es sensible al estadoMás información
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Número de catálogo
Cantidad
D250
100 mg
Número de catálogo D250
Precio (CLP)
405.941
Each
Añadir al carro de la compra
Cantidad:
100 mg
Precio (CLP)
405.941
Each
Añadir al carro de la compra
Cuando el laurdan de la sonda lipofílica se incorpora a las membranas, su espectro de fluorescencia es sensible al estado físico de los fosfolípidos que los rodean
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorVerde
Método de detecciónFluorescente
Para utilizar con (equipo)Microscopio de fluorescencia
Peso molecular353.55
Cantidad100 mg
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaFluorescent Dye
Tipo de productoLaurdan
SubCellular LocalizationMembranas celulares y lípidos, Lipids
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.
Preguntas frecuentes
What is the recommended solvent and concentration for Laurdan (6-Dodecanoyl-2-Dimethylaminonaphthalene) (Cat. No. D250) stock solution?
We recommend resuspending Laurdan (6-Dodecanoyl-2-Dimethylaminonaphthalene) (Cat. No. D250) in dimethylformamide (DMF) or acetonitrile at a concentration of 1 mM. The molecular weight for this product is 353.55 g/mol.
The product is stable at room temperature and should be protected from light.
Two-photon fluorescence microscopy observation of shape changes at the phase transition in phospholipid giant unilamellar vesicles.
Authors:Bagatolli LA, Gratton E
Journal:Biophys J
PubMed ID:10512829
'Using the sectioning effect of the two-photon fluorescence microscope, we studied the behavior of phospholipid giant unilamellar vesicles (GUVs) composed of pure diacylphosphatidylcholine phospholipids during the gel-to-liquid crystalline phase transition. We used the well-characterized excitation generalized polarization function (GP(ex)) of 6-dodecanoyl-2-dimethylamine-naphthalene (LAURDAN), which is sensitive to the changes in water ... More
Physical properties of erythrocyte ghosts that determine susceptibility to secretory phospholipase A2.
Authors:Harris FM, Smith SK, Bell JD
Journal:J Biol Chem
PubMed ID:11294853
'Artificial membranes may be resistant or susceptible to catalytic attack by secretory phospholipase A(2) (sPLA(2)) depending on the physical properties of the membrane. Living cells are normally resistant but become susceptible during trauma, apoptosis, and/or a significant elevation of intracellular calcium. Intact erythrocytes and ghosts were studied to determine whether ... More
Comparative benzene-induced fatty acid changes in a Rhodococcus species and its benzene-sensitive mutant: possible role of myristic and oleic acids in tolerance.
Authors:Gutiérrez T, Learmonth RP, Nichols PD, Couperwhite I
Journal:J Chem Ecol
PubMed ID:14682518
'A Gram positive bacterium of the genus Rhodococcus was isolated from a contaminated site in Sydney, Australia, for its ability to tolerate and degrade high concentrations of benzene. To identify fatty acids that may impart this Rhodococcus sp. with tolerance to toxic solvents, a benzene-sensitive strain, labeled M2b, was isolated ... More
Interactions of Laurdan with phosphatidylcholine liposomes: a high pressure FTIR study.
Authors:Chong PL, Wong PT
Journal:Biochim Biophys Acta
PubMed ID:8323945
'The interactions of 6-lauroyl-2-dimethylaminonaphthalene (Laurdan) with L-alpha-dimyristoylphosphatidylcholine (DMPC) have been studied isothermally at 28 degrees C by Fourier-transform infrared spectroscopy (FTIR) at two pH values (6.8 and 3.0) and over the pressure range of 0.001-25 kbar. The results obtained with Laurdan are compared with those previously obtained with 6-propionyl-2-dimethylaminonaphthalene (Prodan) ... More
Lipid rafts reconstituted in model membranes.
Authors:Dietrich C, Bagatolli LA, Volovyk ZN, Thompson NL, Levi M, Jacobson K, Gratton E
Journal:Biophys J
PubMed ID:11222302
'One key tenet of the raft hypothesis is that the formation of glycosphingolipid- and cholesterol-rich lipid domains can be driven solely by characteristic lipid-lipid interactions, suggesting that rafts ought to form in model membranes composed of appropriate lipids. In fact, domains with raft-like properties were found to coexist with fluid ... More