OxyBURST™ Green H2DCFDA, SE
OxyBURST™ Green H2DCFDA, SE
Citas y referencias (19)
Invitrogen™

OxyBURST™ Green H2DCFDA, SE

El éster de succinimidilo OxyBURST™ Green H2DCFDA (diacetato de 2',7'-diclorodihidrofluoresceína, SE) es un reactivo de ensayo aminorreactivo que puede utilizarseMás información
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Número de catálogoCantidad
D29355 mg
Número de catálogo D2935
Precio (CLP)
224.753
Each
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Cantidad:
5 mg
Precio (CLP)
224.753
Each
Añadir al carro de la compra
El éster de succinimidilo OxyBURST™ Green H2DCFDA (diacetato de 2',7'-diclorodihidrofluoresceína, SE) es un reactivo de ensayo aminorreactivo que puede utilizarse para preparar conjugados sensibles a la oxidación de una amplia variedad de biomoléculas y partículas, incluidos anticuerpos, antígenos, péptidos, proteínas, dextranos, bacterias, levaduras y microesferas de poliestireno. Después de la conjugación con aminas, los dos acetatos de OxyBURST™ Green H2DCFDA pueden eliminarse mediante tratamiento con hidroxilamina con pH neutro para producir el conjugado de dihidrofluoresceína. Los conjugados de OxyBURST™ Green H2DCFDA no son fluorescentes hasta que se oxidan a los correspondientes derivados de la fluoresceína.

Especificaciones del indicador ROS:
• Ex/Em: ∼492–495/517–527 nm
• Este producto es sensible al aire y se debe almacenar bajo nitrógeno o argón seco
• Este producto se puede disolver en DMSO, DMF o etanol para su uso
• El indicador es capaz de penetrar en la célula (en la documentación, se incluyen protocolos de carga de células)
• La fluorescencia puede monitorizarse mediante un citómetro de flujo, un fluorímetro, un lector de microplacas o un microscopio de fluorescencia a través de fuentes de excitación y filtros adecuados para la fluoresceína

Busque más indicadores de ROS
Ofrecemos un surtido de productos de Molecular Probes™ para la generación de especies reactivas de oxígeno (ROS), incluido el oxígeno singlete, el superóxido, el radical hidroxilo y diversos peróxidos e hidroperóxidos, así como para su detección fluorimétrica en solución. Consulte el apartado de generación y detección de especies reactivas de oxígeno (sección 18.2) en el manual de Molecular Probes™ para obtener más información sobre estos productos.

Para uso en investigación. No apto para uso diagnóstico o terapéutico en humanos ni en animales.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Cantidad5 mg
Tipo de productoROS Indicator
Unit SizeEach

Preguntas frecuentes

I want to assay cells for reactive oxygen species using carboxy-H2DCFDA, but I want to do so with a plate reader instead of microscope. Will it work?

It has been done. The problem is that plate readers are less sensitive than microscopes, with far less signal-to-background difference. It is worth trying, but first optimize concentrations and loading times with control cells, use a plate with little to no autofluorescence, and possibly optimize the gain setting in order to get the best signal possible. But don't expect the same sensitivity, even with optimization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have GFP-transfected cells and need to label for reactive oxygen species. Can I use H2DCFDA?

This is not recommended as the two dyes overlap in the emission wavelength. There are other ROS reagents available in different wavelengths, such as CellROX Deep Red, which emits in the far-red range (665 nm), or dihydroethidium, which is emits in the visible red range (620 nm).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cell with CM-H2DCFDA for reactive oxygen detection, but upon illuminating the cell there is a significant increase in fluorescence in the control cells. Why?

If the cell is overloaded with dye, the high intracellular concentration of the dye may lead to dye-dye quenching. Upon illumination, photobleaching will occur, which will reduce the dye-dye quenching and actually increase the fluorescence (for a while, but then it will start decreasing). To solve the problem, reduce the concentration and incubation time, and try a range of incubation times and concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a formaldehyde-fixable reactive oxygen species detection assay. Is H2 DCFDA fixable?

H2DCFDA and similar derivatives are not fixable. The same goes for dihydroethidium and dihydrorhodamine. However, CellROX Deep Red and CellROX Green are retained for a limited time upon fixation with formaldehyde. CellROX Green may be retained upon subsequent Triton X-100 permeabilization. Avoid the use of any acetone or alcohol-based fixatives or fixatives that include alcohol, such as formalin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (19)

Citations & References
Abstract
Effect of C1q on the processing of immune complexes by human neutrophils.
Authors:Ohkuro M, Kobayashi K, Takahashi K, Nagasawa S
Journal:Immunology
PubMed ID:7835978
'We prepared immune complexes (IC) composed of human anti-tetanus toxoid IgG and tetanus toxoid, and examined the effect of C1q on the processing of IC by human neutrophils. Treating IC with increasing amounts of C1q enhanced the binding and phagocytosis of IC by neutrophils, unless the amounts of C1q added ... More
Initial cytoplasmic and phagosomal consequences of human neutrophil exposure to Staphylococcus epidermidis.
Authors:Bernardo J, Long HJ, Simons ER,
Journal:Cytometry A
PubMed ID:19937952
'Microorganisms are recognized by specific phagocyte surface receptors. Liganded receptors then signal a series of events leading to phagocytosis and destruction of the organism by oxidative, lytic, and associated processes. Some organisms, such as Mycobacterium tuberculosis (Mtb), Cryptococcus neoformans (Cf), and others, evade such destruction, surviving and sometimes multiplying within ... More
Stable accumulation of p67phox at the phagosomal membrane and ROS production within the phagosome.
Authors:Tlili A, Erard M, Faure MC, Baudin X, Piolot T, Dupré-Crochet S, Nüsse O,
Journal:J Leukoc Biol
PubMed ID:21954286
'Production of ROS by the leukocyte NADPH oxidase is essential for the destruction of pathogenic bacteria inside phagosomes. The enzyme is a complex of cytosolic and membranous subunits that need to assemble upon activation. Biochemical data suggest that the complex is renewed continuously during activity. Furthermore, it is generally assumed ... More
Reactive oxygen species-producing site in radiation and hydrogen peroxide-induced apoptosis of human peripheral T cells: Involvement of lysosomal membrane destabilization.
Authors:Ogawa Y, Kobayashi T, Nishioka A, Kariya S, Ohnishi T, Hamasato S, Seguchi H, Yoshida S
Journal:Int J Mol Med
PubMed ID:15067365
'In our previous studies, we showed that the apoptotic resistance of the human osteosarcoma cell line HS-Os-1 against irradiation was easily converted to a state of apoptotic-susceptibility by the addition of a relatively low concentration of hydrogen peroxide to the culture medium just prior to irradiation. When we consider the ... More
Malaria inhibits surface expression of complement receptor 1 in monocytes/macrophages, causing decreased immune complex internalization.
Authors:Fernandez-Arias C, Lopez JP, Hernandez-Perez JN, Bautista-Ojeda MD, Branch O, Rodriguez A,
Journal:J Immunol
PubMed ID:23440418
Complement receptor 1 (CR1) expressed on the surface of phagocytic cells binds complement-bound immune complexes (IC), playing an important role in the clearance of circulating IC. This receptor is critical to prevent accumulation of IC, which can contribute to inflammatory pathology. Accumulation of circulating IC is frequently observed during malaria, ... More
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