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Citas y referencias (31)
Invitrogen™
β-BODIPY™ FL C12-HPC (2-(4,4-difluoro-5,7-dimetil-4-bora-3a,4a-diaza-s-indaceno-3-dodecanoil)-1-hexadecanoil-sn-glicero-3-fosfocolina)
El fluoróforo BODIPY® (4,4-difluoro-3a,4a-diaza-s-indacene) es intensamente fluorescente y lipofílico a diferencia de la mayoría de los colorantes de longitud deMás información
| Número de catálogo | Cantidad |
|---|---|
| D3792 | 100 μg |
Número de catálogo D3792
Precio (CLP)
178.691
Each
Cantidad:
100 μg
Precio (CLP)
178.691
Each
El fluoróforo BODIPY® (4,4-difluoro-3a,4a-diaza-s-indacene) es intensamente fluorescente y lipofílico a diferencia de la mayoría de los colorantes de longitud de onda prolongada. Por consiguiente, es más probable que las sondas que incorporan este fluoróforo imiten las propiedades de los lípidos naturales. Los analógicos de ácidos grasos BODIPY® como el ácido dodecanoico BODIPY® 500/510 (D-3823) y el ácido dodecanoico BODIPY FL (D-3822) experimentan un transporte nativo y metabolismo en las células. Por lo tanto, son trazadores eficaces de tráfico de lípidos, siendo también sondas de membrana útiles de uso general.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Nombre del producto químico o materialFosfolípidos
Almacenamiento recomendadoAlmacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Forma físicaSolid
Línea de productosBODIPY
Cantidad100 μg
Unit SizeEach
Citations & References (31)
Citations & References
Abstract
Separation and characterization of late endosomal membrane domains.
Journal:J Biol Chem
PubMed ID:12065580
'Very little is known about the biophysical properties and the lipid or protein composition of membrane domains presumably present in endocytic and biosynthetic organelles. Here we analyzed the membrane composition of late endosomes by suborganellar fractionation in the absence of detergent. We found that the internal membranes of this multivesicular
Multidrug transporter activity in lymphocytes.
Journal:Br J Pharmacol
PubMed ID:15492020
'Multidrug transporters play a dual role in haematopoietic cells, mediating the efflux of xenobiotics and regulating cell migration. For several reasons including the lack of specific antibodies, reports of multidrug transporter distribution on lymphocytes conflict. Murine B cells have been reported to completely lack transporter activity. Through analysis of parental
gamma-cyclodextrins greatly enhance translocation of hydrophobic fluorescent phospholipids from vesicles to cells in culture. Importance of molecular hydrophobicity in phospholipid trafficking studies.
Journal:J Biol Chem
PubMed ID:10585403
'Short-chain, fluorescent derivatives are commonly used to investigate intracellular phospholipid trafficking. However, their use can yield misleading results because they, unlike the native species, can rapidly distribute between organelles due to their low hydrophobicity. On the other hand, hydrophobic derivatives are very difficult to introduce to cells and thus have
Fluorescent membrane probes incorporating dipyrrometheneboron difluoride fluorophores.
Journal:Anal Biochem
PubMed ID:1799206
'The spectroscopic properties of a new series of fatty acid analogs in which a dipyrrometheneboron difluoride fluorophore forms a segment of the acyl methylene chain are presented and their characteristics as fluorescent membrane probes are examined. When incorporated as a low mole fraction component in model phospholipid membranes, the probes
Intracellular surfactant removal from phagocytized minerals: development of a fluorescent method using a BODIPY-labeled phospholipid.
Journal:Inhal Toxicol
PubMed ID:10880156
'Lung surfactant serves as a protective coating when adsorbed on particle surfaces, so its removal or rate of removal in vivo may affect expression of mineral cytotoxicity. Removal of phospholipid surfactant components from the surface of mineral particles ingested by alveolar macrophages (AM) was measured using fluorescence microscopy. Dipalmitoylphosphatidylcholine with