H2DCFDA (H2-DCF, DCF)
H2DCFDA (H2-DCF, DCF)
Citas y referencias (1016)
Invitrogen™

H2DCFDA (H2-DCF, DCF)

El diacetato de 2',7'-diclorodihidrofluoresceína (H2DCFDA) (también denominado diacetato de diclorofluores ceína) penetra en la célula. Se trata de una formaMás información
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Número de catálogoCantidad
D399100 mg
Número de catálogo D399
Precio (CLP)
206.931
Each
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Cantidad:
100 mg
Precio (CLP)
206.931
Each
Añadir al carro de la compra
El diacetato de 2',7'-diclorodihidrofluoresceína (H2DCFDA) (también denominado diacetato de diclorofluores ceína) penetra en la célula. Se trata de una forma de fluoresceína reducida químicamente que se utiliza como indicador de especies reactivas de oxígeno (ROS) en las células, por ejemplo, para detectar la generación de productos intermediarios reactivos de oxígeno en neutrófilos y macrófagos. Tras la disociación de los grupos de acetato por parte de las esterasas intracelulares y la oxidación, el H2DCFDA no fluorescente se convierte en 2',7'-diclorofluoresceína (DCF), que es altamente fluorescente. Hay disponibles versiones de este indicador con mayor nivel de retención celular (C400, C2938).

Especificaciones del indicador ROS:

• Ex/Em: ∼ 492–495/517–527 nm
• Este producto es sensible al aire y se debe almacenar bajo nitrógeno o argón seco
• Este producto se puede disolver en DMSO, DMF o etanol para su uso
• El indicador es capaz de penetrar en la célula (en la documentación, se incluyen protocolos de carga de células)
• La fluorescencia puede monitorizarse mediante un citómetro de flujo, un fluorímetro, un lector de microplacas o un microscopio de fluorescencia a través de fuentes de excitación y filtros adecuados para la fluoresceína

Encuentre más indicadores de ROS
Ofrecemos un surtido de productos de Molecular Probes™ para la generación de especies reactivas de oxígeno (ROS), incluido el oxígeno singulete, el superóxido, el radical hidroxilo y diversos peróxidos e hidroperóxidos, así como para su detección fluorimétrica en solución. Consulte el apartado de generación y detección de especies reactivas de oxígeno (sección 18.2) en el manual de Molecular Probes™ para obtener más información sobre estos productos.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Cantidad100 mg
Tipo de productoROS Indicator
Unit SizeEach

Preguntas frecuentes

I want to assay cells for reactive oxygen species using carboxy-H2DCFDA, but I want to do so with a plate reader instead of microscope. Will it work?

It has been done. The problem is that plate readers are less sensitive than microscopes, with far less signal-to-background difference. It is worth trying, but first optimize concentrations and loading times with control cells, use a plate with little to no autofluorescence, and possibly optimize the gain setting in order to get the best signal possible. But don't expect the same sensitivity, even with optimization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have GFP-transfected cells and need to label for reactive oxygen species. Can I use H2DCFDA?

This is not recommended as the two dyes overlap in the emission wavelength. There are other ROS reagents available in different wavelengths, such as CellROX Deep Red, which emits in the far-red range (665 nm), or dihydroethidium, which is emits in the visible red range (620 nm).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cell with CM-H2DCFDA for reactive oxygen detection, but upon illuminating the cell there is a significant increase in fluorescence in the control cells. Why?

If the cell is overloaded with dye, the high intracellular concentration of the dye may lead to dye-dye quenching. Upon illumination, photobleaching will occur, which will reduce the dye-dye quenching and actually increase the fluorescence (for a while, but then it will start decreasing). To solve the problem, reduce the concentration and incubation time, and try a range of incubation times and concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a formaldehyde-fixable reactive oxygen species detection assay. Is H2 DCFDA fixable?

H2DCFDA and similar derivatives are not fixable. The same goes for dihydroethidium and dihydrorhodamine. However, CellROX Deep Red and CellROX Green are retained for a limited time upon fixation with formaldehyde. CellROX Green may be retained upon subsequent Triton X-100 permeabilization. Avoid the use of any acetone or alcohol-based fixatives or fixatives that include alcohol, such as formalin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the recommended storage condition/shelf-life for H2DCFDA (H2-DCF, DCF) (Cat. No. D399)?

We guarantee stability of the product for a period of 3 months when stored as recommended in the freezer (-5 to -30 degrees C).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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Citations & References (1016)

Citations & References
Abstract
Nitric oxide protects cultured rat hepatocytes from tumor necrosis factor-alpha-induced apoptosis by inducing heat shock protein 70 expression.
Authors:Kim YM,de Vera ME,Watkins SC,Billiar TR
Journal:The Journal of biological chemistry
PubMed ID:8995451
Differing effects of copper,zinc superoxide dismutase overexpression on neurotoxicity elicited by nitric oxide, reactive oxygen species, and excitotoxins.
Authors:Ying W,Anderson CM,Chen Y,Stein BA,Fahlman CS,Copin JC,Chan PH,Swanson RA
Journal:Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
PubMed ID:10698074
Authors:
Journal:
PubMed ID:11282963
Imaging of hydroperoxides in a rat glomerulus stimulated by puromycin aminonucleoside.
Authors:Aoyagi K,Akiyama K,Tomida C,Gotoh M,Hirayama A,Takemura K,Ueda A,Nagase S,Koyama A,Narita M
Journal:Kidney international. Supplement
PubMed ID:10412763
Cellular alterations produced by the experimental increase in intracellular calcium and the nature of protective effects from pretreatment with nimodipine.
Authors:Danks AM,Hammond DN,Wainer BH,Van Buskirk RG,Isaacson RL
Journal:Brain research. Molecular brain research
PubMed ID:1334195
The immortalized septal cell line, SN56 B5 G4, generated by the fusion of mouse septal area cells and neuroblastoma cells, was used to determine if nimodipine, an antagonist of voltage sensitive calcium 'L' channels, might act in a neuroprotective fashion when intracellular calcium levels were raised by incubation in ouabain ... More
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