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Citas y referencias (5)
Invitrogen™
BODIPY™ FL Sulfonated Succinimidyl Ester
El éster de sulfosuccinimidina BODIPY® FL, reactivo a la amina, puede utilizarse para crear bioconjugados verde-fluorescentes. Esta fracción reactiva seMás información
| Número de catálogo | Cantidad |
|---|---|
| D6140 | 5 mg |
Número de catálogo D6140
Precio (CLP)
-
Cantidad:
5 mg
El éster de sulfosuccinimidina BODIPY® FL, reactivo a la amina, puede utilizarse para crear bioconjugados verde-fluorescentes. Esta fracción reactiva se sulfona para aumentar la solubilidad en agua. El colorante BODIPY® FL electrónicamente neutro tiene el espectro más parecido a la fluoresceína de los colorantes BODIPY® verdes fluorescentes.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Reactividad químicaAmina
Etiqueta o tinteBODIPY™ FL
Tipo de productoÉster de succinimidilo sulfonado FL
Cantidad5 mg
Fracción reactivaEster activo, succinimidilo éster
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaColorantes BODIPY
Línea de productosBODIPY
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Citations & References (5)
Citations & References
Abstract
Fluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY((R)) FL-labeled probe or primer.
Journal:Nucleic Acids Res
PubMed ID:11239011
We have developed a simple method for the quantitative detection of specific DNA or RNA molecules based on the finding that BODIPY((R)) FL fluorescence was quenched by its interaction with a uniquely positioned guanine. This approach makes use of an oligonucleotide probe or primer containing a BODIPY((R)) FL-modified cytosine at
Ultrasensitive fluorescence-based detection of nascent proteins in gels.
Journal:Anal Biochem
PubMed ID:10706791
The most common method of analysis of proteins synthesized in a cell-free translation system (e.g., nascent proteins) involves the use of radioactive amino acids such as [(35)S]methionine or [(14)C]leucine. We report a sensitive, nonisotopic, fluorescence-based method for the detection of nascent proteins directly in polyacrylamide gels. A fluorescent reporter group
Binding and internalization of lipopolysaccharide by Cla-1, a human orthologue of rodent scavenger receptor B1.
Journal:J Biol Chem
PubMed ID:12651854
Scavenger receptor, class B, type I (SR-BI) mediates selective uptake of high density lipoprotein (HDL) cholesteryl ester. SR-BI recognizes HDL, low density lipoprotein (LDL), exchangeable apolipoproteins, and protein-free lipid vesicles containing negatively charged phospholipids. Lipopolysaccharides (LPS) are highly glycosylated anionic phospholipids contributing to septic shock. Despite significant structural similarities between
Synthesis of fluorescent analogs of alpha-conotoxin MII.
Journal:Bioconjug Chem
PubMed ID:17105243
Alpha-conotoxins (alpha-CTxs) are small peptides that are competitive inhibitors of nicotinic acetylcholine receptors (nAChRs) and have been used to study the kinetics of nAChRs. Alpha-CTx MII, from the venom of Conus magus, has been shown to potently block both rat alpha3beta2 and rat chimeric alpha6/alpha3beta2beta3 cloned nAChRs expressed in Xenopus
A high-throughput nonisotopic protein truncation test.
Journal:Nat Biotechnol
PubMed ID:12524552
Nonsense or frameshift mutations, which result in a truncated gene product, are prevalent in a variety of disease-related genes, including APC (implicated in colorectal cancer), BRCA1 and BRCA2 (breast and ovarian cancer), PKD1 (polycystic kidney disease), NF1 and NF2 (neurofibromatosis), and DMD (Duchenne muscular dystrophy). Such chain-truncating mutations can be