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Citas y referencias (24)
Invitrogen™
Esfingomielina BODIPY™ FL C12 (N-(4,4-difluoro-5,7-dimetil-4-bora-3a,4a-diaza-s-indaceno-3-dodecanoil)esfingosil fosfocolina)
La esfingomielina BODIPY™ FL C12 verde fluorescente puede usarse para estudiar los mecanismos de transporte y metabolismo de esfingolípidos.Más información
| Número de catálogo | Cantidad |
|---|---|
| D7711 | 250 μL |
Número de catálogo D7711
Precio (CLP)
481.090
Each
Cantidad:
250 μL
Precio (CLP)
481.090
Each
La esfingomielina BODIPY™ FL C12 verde fluorescente puede usarse para estudiar los mecanismos de transporte y metabolismo de esfingolípidos.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Nombre del producto químico o materialEsfingolípidos
Almacenamiento recomendadoAlmacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.
Línea de productosBODIPY
Cantidad250 μL
Unit SizeEach
Citations & References (24)
Citations & References
Abstract
Fluorescence-based selection of gene-corrected hematopoietic stem and progenitor cells from acid sphingomyelinase-deficient mice: implications for Niemann-Pick disease gene therapy and the development of improved stem cell gene transfer procedures.
Journal:Blood
PubMed ID:9864149
'The general utility of a novel, fluorescence-based procedure for assessing gene transfer and expression has been demonstrated using hematopoietic stem and progenitor cells. Lineage-depleted hematopoietic cells were isolated from the bone marrow or fetal livers of acid sphingomyelinase-deficient mice, and retrovirally transduced with amphotropic or ecotropic vectors encoding a normal
gamma-cyclodextrins greatly enhance translocation of hydrophobic fluorescent phospholipids from vesicles to cells in culture. Importance of molecular hydrophobicity in phospholipid trafficking studies.
Journal:J Biol Chem
PubMed ID:10585403
'Short-chain, fluorescent derivatives are commonly used to investigate intracellular phospholipid trafficking. However, their use can yield misleading results because they, unlike the native species, can rapidly distribute between organelles due to their low hydrophobicity. On the other hand, hydrophobic derivatives are very difficult to introduce to cells and thus have
A fluorescent glycolipid-binding peptide probe traces cholesterol dependent microdomain-derived trafficking pathways.
Journal:PLoS ONE
PubMed ID:18716682
'BACKGROUND: The uptake and intracellular trafficking of sphingolipids, which self-associate into plasma membrane microdomains, is associated with many pathological conditions, including viral and toxin infection, lipid storage disease, and neurodegenerative disease. However, the means available to label the trafficking pathways of sphingolipids in live cells are extremely limited. In order
A fluorescence-based, high-performance liquid chromatographic assay to determine acid sphingomyelinase activity and diagnose types A and B Niemann-Pick disease.
Journal:Anal Biochem
PubMed ID:12633609
'Acid sphingomyelinase (ASM; sphingomyelin phosphodiesterase, EC 3.1.4.12) is the lysosomal enzyme that hydrolyzes sphingomyelin (SPM) to phosphorylcholine and ceramide. An inherited deficiency of ASM activity results in Types A and B Niemann-Pick disease (NPD). In this study we report a new assay method to detect ASM activity and diagnose NPD
Synthesis of fluorescent substrates and their application to study of sphingolipid metabolism in vitro and in intact cells.
Journal:Methods Enzymol
PubMed ID:11070879