SP-DiOC₁₈(3) (3,3'-Dioctadecyl-5,5'-Di(4-Sulfophenyl)Oxacarbocyanine, Sodium Salt)

SP-DiOC<sub>18</sub>(3) (3,3'-Dioctadecyl-5,5'-Di(4-Sulfophenyl)Oxacarbocyanine, Sodium Salt)

Citas y referencias (11)

Invitrogen™

SP-DiOC₁₈(3) (3,3'-Dioctadecyl-5,5'-Di(4-Sulfophenyl)Oxacarbocyanine, Sodium Salt)

La carbocianina lipofílica SP-DiOC18(3) con fluorescencia de verde es un analógico DiO que contiene grupos de sulfonatos para mejorar laMás información

Número de catálogo	Cantidad
D7778	5 mg

Número de catálogo D7778

Precio (CLP)

455.890

Añadir al carro de la compra

Cantidad:

Precio (CLP)

455.890

Añadir al carro de la compra

La carbocianina lipofílica SP-DiOC₁₈(3) con fluorescencia de verde es un analógico DiO que contiene grupos de sulfonatos para mejorar la solubilidad en agua. Es débilmente fluorescente en agua, pero muy fluorescente y bastante fotoestable cuando se incorpora a membranas. Los grupos de sulfonatos incorporados en este analógico del DiI mejoran la solubilidad en agua. Tiene un coeficiente de extinción extremadamente alto y tiempos de vida de estado excitado cortos (∼ 1 nanosegundo) en entornos lipídicos. Una vez aplicado a las células, el colorante se difunde lateralmente dentro de la membrana plasmática.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorVerde

Método de detecciónFluorescente

Para utilizar con (equipo)Microscopio de fluorescencia

Cantidad5 mg

Condiciones de envíoTemperatura ambiente

Tipo de etiquetaFluorescent Dye

Tipo de productoSP-DiOC18(3)

SubCellular LocalizationMembrana plasmática

Unit SizeEach

Contenido y almacenamiento

Almacenar a temperatura ambiente y proteger de la luz.

Preguntas frecuentes

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label two cell populations and then perform a cell fusion assay. Which reagents are best for imaging this?

Lipophilic cyanine dyes are preferred for this sort of assay, since they insert into cellular membranes and then, upon fusion, are shared by the fused cells as the membranes are shared. For example, one cell population can be labeled with DiI (orange-red) and another cell population can be labeled with DiO (green), and when the cells fuse, the combined color appears yellow (when imaged with a dual-bandpass filter set).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (11)

Citations & References

Abstract

Dynamic behavior of cells within neurospheres in expanding populations of neural precursors.

Authors:Wang TY, Sen A, Behie LA, Kallos MS,

Journal:Brain Res

PubMed ID:16859652

'Large-scale expansion of neural stem and progenitor cells will be essential for clinically treating the large number of patients suffering from neurodegenerative disorders such as Parkinson''s disease. Other applications of neural stem cell technology include further research in areas such as neural development or drug testing. Neural stem cells can ... More

Interaction of Actinobacillus actinomycetemcomitans outer membrane vesicles with HL60 cells does not require leukotoxin.

Authors:Demuth DR, James D, Kowashi Y, Kato S

Journal:Cell Microbiol

PubMed ID:12580947

'Outer membrane derived vesicles (MVs) secreted by Actinobacillus actinomycetemcomitans JP2 contain a membranolytic leukotoxin and are toxic to human HL60 cells. To determine how MVs interact with human target cells, HL60 cells were incubated with vesicles, reacted with anti-vesicle antibodies and a FITC-labelled reporter, and visualized by confocal scanning laser ... More

Use of vascular explants for ex vivo neovascularization of biomaterials.

Authors:Iurlaro M, Sanders JE, Zhu WH, Scatena M, Mitchell SB, Nicosia RF

Journal:Microvasc Res

PubMed ID:12453434

'Biomaterial polymers have been proposed as scaffolds for cell assembly in vascular bioengineering. We describe here a new method for the neovascularization of polyurethane meshes from explants of rat aorta. Aortic rings embedded in collagen-permeated polyurethane meshes and cultured in medium supplemented with fetal bovine serum and vascular endothelial growth ... More

Radiation increases invasion of gene-modified mesenchymal stem cells into tumors.

Authors:Zielske SP, Livant DL, Lawrence TS,

Journal:Int J Radiat Oncol Biol Phys

PubMed ID:18849123

Mesenchymal stem cells (MSCs) are multipotent cells in the bone marrow that have been found to migrate to tumors, suggesting a potential use for cancer gene therapy. MSCs migrate to sites of tissue damage, including normal tissues damaged by radiation. In this study, we investigated the effect of tumor radiotherapy ... More

Suitability of various membrane lipophilic probes for the detection of trogocytosis by flow cytometry.

Authors:Daubeuf S, Bordier C, Hudrisier D, Joly E,

Journal:Cytometry A

PubMed ID:19051238

Trogocytosis is a recently discovered phenomenon whereby lymphocytes capture fragments of the plasma membrane from antigen presenting cells (APCs). Using APCs labeled with widely used fluorescent lipophilic probes, we previously described a trogocytosis analysis protocol (TRAP) useful to understand the mechanisms and biological consequences of this process and to identify ... More

View all SP-DiOC₁₈(3) (3,3'-Dioctadecyl-5,5'-Di(4-Sulfophenyl)Oxacarbocyanine, Sodium Salt) citations

SP-DiOC18(3) (3,3'-Dioctadecyl-5,5'-Di(4-Sulfophenyl)Oxacarbocyanine, Sodium Salt)

Preguntas frecuentes

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

I want to label two cell populations and then perform a cell fusion assay. Which reagents are best for imaging this?

Citations & References (11)

SP-DiOC₁₈(3) (3,3'-Dioctadecyl-5,5'-Di(4-Sulfophenyl)Oxacarbocyanine, Sodium Salt)