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Citas y referencias (52)
Invitrogen™
Ethidium Homodimer-2 (EthD-2) - 1 mM Solution in DMSO
El homodímero-2 de etidio (EthD-2) es una indicador de viabilidad de permeabilidad celular de ácido nucleico de alta afinidad queMás información
| Número de catálogo | Cantidad |
|---|---|
| E3599 | 200 μl |
Número de catálogo E3599
Precio (CLP)
462.763
Each
Cantidad:
200 μl
Precio (CLP)
462.763
Each
El homodímero-2 de etidio (EthD-2) es una indicador de viabilidad de permeabilidad celular de ácido nucleico de alta afinidad que es débilmente fluorescente hasta que se une al ADN y emite fluorescencia roja (excitación/emisión máximas de ∼535/624).
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorNaranja
DescripciónHomodímero de etidio-2 (EthD-2): solución de 1 mM en DMSO
Método de detecciónFluorescente
Tipo de coloranteImpenetrable en células
Emisión624 nm
Intervalo de longitud de onda de excitación535 nm
Para utilizar con (aplicación)Ensayos de tinción celular
Para utilizar con (equipo)Microscopio de fluorescencia
Cantidad200 μl
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaFluorescent Dye
Tipo de productoHomodímero de etidio-2
SubCellular LocalizationNucleicos, ácidos nucleicos, Nucleus
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Citations & References (52)
Citations & References
Abstract
Differential effects of deuterium oxide on the fluorescence lifetimes and intensities of dyes with different modes of binding to DNA.
Journal:J Histochem Cytochem
PubMed ID:9016307
'Deuterium oxide (D2O) increases both the fluorescence lifetime and the fluorescence intensity of the intercalating dyes propidium iodide (PI) and ethidium bromide (EB) when bound to nucleic acid structures. We have used spectroscopic analysis coupled with conventional and phase-sensitive flow cytometry to compare the alterations in intensity and lifetime of
Diverse microglial motility behaviors during clearance of dead cells in hippocampal slices.
Journal:Glia
PubMed ID:15042586
'We used two-channel three-dimensional time-lapse fluorescence confocal imaging in live rat hippocampal slice cultures (1-7 days in vitro) to determine the motility behaviors of activated microglia as they engage dead and dying cells following traumatic brain tissue injury. Live microglia were labeled with a fluorescently conjugated lectin (IB(4)), and dead
Calmodulin antagonists differentially affect capacitation-associated protein tyrosine phosphorylation of mouse sperm components.
Journal:J Cell Sci
PubMed ID:12668727
'Sperm capacitation in vitro is thought to be correlated with the increased protein tyrosine phosphorylation of a subset of sperm components. Our group recently used a pharmacological approach to demonstrate that calmodulin (CaM), a 17 kDa calcium sensor protein, has a role in sperm capacitation. In the present study, we
Automated image analysis of live/dead staining of the fungus Aureobasidium pullulans on microscope slides and leaf surfaces.
Journal:Biotechniques
PubMed ID:11056819
'An image analysis program and protocol for the identification and enumeration of live versus dead cells of the yeast-like fungus Aureobasidium pullulans was developed for both populations on microscope slides and leaf surfaces. Live cells took up CellTracker Blue, while nonviable cells stained with DEAD Red. Image analysis macro programs
A2E-epoxides damage DNA in retinal pigment epithelial cells. Vitamin E and other antioxidants inhibit A2E-epoxide formation.
Journal:J Biol Chem
PubMed ID:12646558
'The autofluorescent pigments that accumulate in retinal pigment epithelial cells with aging and in some retinal disorders have been implicated in the etiology of macular degeneration. The major constituent is the fluorophore A2E, a pyridinium bisretinoid. Light-exposed A2E-laden retinal pigment epithelium exhibits a propensity for apoptosis with light in the