Geles TBE Novex™, 4-12 %, 12 pocillos

Citas y referencias (15)

Invitrogen™

Geles TBE Novex™, 4-12 %, 12 pocillos

Los geles de Tris-borato-EDTA (TBE) Novex™ proporcionan la máxima resolución posible para analizar digestiones de restricción y productos de PCR.Más información

Número de catálogo	Cantidad
EC62352BOX	1 caja

Número de catálogo EC62352BOX

Precio (CLP)

Cantidad:

1 caja

Pedido a granel o personalizado

Los geles de Tris-borato-EDTA (TBE) Novex™ proporcionan la máxima resolución posible para analizar digestiones de restricción y productos de PCR. Los fragmentos de ADN se resuelven con claridad en bandas nítidas y ajustadas. Los geles de Novex™ TBE están diseñados para ejecutarse en la minicelda XCell SureLock™

Formulación
Los geles de Novex™ TBE se fabrican con una base de Tris de alta pureza, ácido bórico, EDTA, acrilamida, bisacrilamida, TEMED y APS.

Tampones recomendados
Para un rendimiento óptimo, con estos geles se recomienda el uso del tampón de desplazamiento Novex™ TBE y el tampón de muestras de alta densidad Novex™ TBE. El tampón de muestras de TBE de alta densidad Novex™ contiene el agente de densidad FICOLL™, que genera bandas más nítidas y rectas que los agentes de densidad tradicionales, y los tintes de seguimiento azul de bromofenol y xileno cianol.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

DescripciónNovex TBE Gels, 4 to 12%, 12-well

Tipo de productoGel de TBE

Cantidad1 caja

Tipo de muestraDouble-stranded DNA (dsDNA)

Condiciones de envíoHielo húmedo

Para utilizar con (equipo)XCell SureLock Mini-Cell

Porcentaje del gelDel 4 al 12%

Tipo de gelTBE

Intervalo de separación35 to 400 bp

Pocillos12 pocillo

Unit SizeEach

Contenido y almacenamiento

• El número de catálogo hace referencia a una sola caja de 10 geles

Almacenar a 4 °C.

Preguntas frecuentes

Can I stain my TBE gel? How?

Yes, you can stain your TBE gels with ethidium bromide, SYBR Green I, SYBR Green II, and the SilverXpress Silver Staining Kit. For ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light.

What are the smallest fragments that can be visualized on the TBE gels?

On the Invitrogen 10% TBE gels, a 51 bp marker can be clearly seen. On the Invitrogen 20% TBE gel, the 18 and 12 bp markers can be clearly seen.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the concentration of EDTA in the TBE gels? How about glycerol?

The EDTA concentration in our TBE gels is 0.06% (w/v). The 20% TBE gels contain 4% glycerol for maximal resolution. All other TBE gels contain 0.8% glycerol in a layer that represents the bottom 9% of the gel. There is no glycerol in the rest of the gel.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I stain my TBE gel or my TBE-urea gel? How?

Yes, for ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What should the running conditions be for the TBE gels (voltage, current, run time, etc.)?

Voltage: 200 V constant*
Approximate current at start: 10-18 mA/gel
Approximate current at end: 4-6 mA/gel
Run time: Approximately 30-90 minutes, dependent on gel percentage. The run is complete when the bromophenol blue (darker) tracking dye reaches the bottom of the gel.

* Voltages up to 250 V may be used to reduce run time.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

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Citations & References (15)

Citations & References

Abstract

Prevalence and correlates of GB virus C infection in HIV-infected and HIV-uninfected pregnant women in Bangkok, Thailand.

Authors:Bhanich Supapol W, Remis RS, Raboud J, Millson M, Tappero J, Kaul R, Kulkarni P, McConnell MS, Mock PA, McNicholl JM, Roongpisuthipong A, Chotpitayasunondh T, Shaffer N, Butera S

Journal:J Med Virol

PubMed ID:21108337

'GB virus C (GBV-C) is an apathogenic virus that has been shown to inhibit HIV replication. This study examined the prevalence and correlates of GBV-C infection and clearance in three cohorts of pregnant women in Thailand. The study population consisted of 1,719 (1,387 HIV-infected and 332 HIV-uninfected) women from three ... More

An in vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides.

Authors:Datta K, Purkayastha S, Neumann RD, Winters TA

Journal:Methods Mol Biol

PubMed ID:22941624

'DNA double-strand breaks (DSBs) are caused by endogenous cellular processes such as oxidative metabolism, or by exogenous events like exposure to ionizing radiation or other genotoxic agents. Repair of these DSBs is essential for the maintenance of cellular genomic integrity. In human cells, and cells of other higher eukaryotes, DSBs ... More

Smooth muscle phenotypic diversity is mediated through alterations in myocardin gene splicing.

Authors:Ilagan RM, Genheimer CW, Quinlan SF, Guthrie KI, Sangha N, Ramachandrannair S, Kelley RW, Presnell SC, Basu J, Ludlow JW

Journal:J Cell Physiol

PubMed ID:21792927

'Myocardin (MYOCD) is a smooth and cardiac muscle-specific transcriptional coactivator that is required for the proper expression of contraction-related genes. Through its function to transactivate effector genes, MYOCD plays an essential role in mediating the switch between contractile and non-contractile phenotypes, particularly in smooth muscle cells (SMC). There are at ... More

Genome wide full-length transcript analysis using 5' and 3' paired-end-tag next generation sequencing (RNA-PET).

Authors:Ruan X, Ruan Y

Journal:Methods Mol Biol

PubMed ID:22113299

'RNA-PET is a paired end tag (PET) sequencing method for full-length mRNA transcripts analysis using the next generation sequencer platforms such as Illumina GA and SOLiD. Unlike RNA-Seq method that sequences randomly sheared shotgun RNA short fragments, RNA-PET captures and sequences the 5'' and 3'' end tags of full-length cDNA ... More

Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition.

Authors:Adey A, Morrison HG, Asan Xun X, Kitzman JO, Turner EH, Stackhouse B, MacKenzie AP, Caruccio NC, Zhang X, Shendure J

Journal:Genome Biol

PubMed ID:21143862

We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its ... More

View all Geles TBE Novex™, 4-12 %, 12 pocillos citations