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Citas y referencias (751)
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Fura-2, sal pentapotásica, impenetrable en células
La sal de pentapotasio Fura-2 es un indicador de calcio intracelular de alta afinidad radiométrico y excitable mediante luz UV.Más información
| Número de catálogo | Cantidad |
|---|---|
| F1200 | 1 mg |
Número de catálogo F1200
Precio (CLP)
278.345
Each
Cantidad:
1 mg
Precio (CLP)
278.345
Each
La sal de pentapotasio Fura-2 es un indicador de calcio intracelular de alta afinidad radiométrico y excitable mediante luz UV. Esta forma de sal soluble en agua es útil para la carga intracelular mediante microinyección, infusión a partir de una pipeta de parche o absorción inducida por nuestro reactivo de carga celular pinocítica Influx(I14402).
Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de membrana ›
Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de membrana ›
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteA base de colorantes fluorescentes
Cantidad1 mg
Condiciones de envíoTemperatura ambiente
Para utilizar con (equipo)Microscopio de fluorescencia
Tipo de productoFura-2, sal pentapotásica
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.
Citations & References (751)
Citations & References
Abstract
Simultaneous measurement of intracellular pH and Ca2+ in insulin-secreting cells by spectral imaging microscopy.
Journal:The American journal of physiology
PubMed ID:8967445
Arabidopsis abi1-1 and abi2-1 phosphatase mutations reduce abscisic acid-induced cytoplasmic calcium rises in guard cells.
Journal:The Plant cell
PubMed ID:10488243
Elevations in cytoplasmic calcium ([Ca(2)+](cyt)) are an important component of early abscisic acid (ABA) signal transduction. To determine whether defined mutations in ABA signal transduction affect [Ca(2)+](cyt) signaling, the Ca(2)+-sensitive fluorescent dye fura 2 was loaded into the cytoplasm of Arabidopsis guard cells. Oscillations in [Ca(2)+](cyt) could be induced when
Ca2+ transients in cardiac myocytes measured with high and low affinity Ca2+ indicators.
Journal:Biophysical journal
PubMed ID:8274651
Intracellular calcium ion ([Ca2+]i) transients were measured in voltage-clamped rat cardiac myocytes with fura-2 or furaptra to quantitate rapid changes in [Ca2+]i. Patch electrode solutions contained the K+ salt of fura-2 (50 microM) or furaptra (300 microM). With identical experimental conditions, peak amplitude of stimulated [Ca2+]i transients in furaptra-loaded myocytes
Evidence for role of cytosolic free calcium in hypoxia-induced proximal tubule injury.
Journal:J Clin Invest
PubMed ID:8182125
The role of cytosolic free Ca2+ ([Ca2+]i) in hypoxic injury was investigated in rat proximal tubules. [Ca2+]i was measured using fura-2 and cell injury was estimated with propidium iodide (PI) in individual tubules using video imaging fluorescence microscopy. [Ca2+]i increased from approximately 170 to approximately 390 nM during 5 min
Hyposmotic activation hyperpolarizes outer hair cells of guinea pig cochlea.
Journal:Brain Res
PubMed ID:8348313
The electrophysiological responses of isolated guinea pig outer hair cells (OHCs) to hyposmotic activation were studied using the whole-cell patch-clamp technique. The cell swelling by hyposmotic activation hyperpolarized OHCs by 6.6 +/- 2.3 mV from the resting membrane potential of -58.5 +/- 5.9 mV (n = 48). This hyperpolarization was