FluoSpheres™ Carboxylate-Modified Microspheres

Citas y referencias (3)

Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Name: FluoSpheres™ Carboxylate-Modified Microspheres
SKU: F20881
Price: 530.345 CLP

Consiga la fluorescencia más brillante gracias a las microesferas FluoSphere modificadas con carboxilos, disponibles en diferentes colores y tamaños de partículas.

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Número de catálogo	Diámetro (métrico)	Color	Excitación/emisión	Cantidad
F20881	0,2 μm	Naranja	365/610 nm	2 mL
F8803	0,1 μm	Amarillo-verde	505/515 nm	10 mL
F8816	1,0 μm	Carmesí	625/645 nm	2 mL
F8823	1,0 μm	Amarillo-verde	505/515 nm	10 mL
F8801	0,1 μm	Rojo	580/605 nm	10 mL
F8811	0,2 μm	Amarillo-verde	505/515 nm	10 mL
F8807	0,2 μm	Rojo oscuro	660/680 nm	2 mL
F10720	0,04 μm	Amarillo-verde, Naranja, Rojo, Rojo oscuro	505/515, 540/560, 580/605, 660/680 nm	1 ml (cada uno)
F8783 también denominado F-8783	0,02 μm	Rojo oscuro	660/680 nm	2 mL
F8786	0,02 μm	Rojo	580/605 nm	10 mL
F8795 también denominado F-8795	0,04 μm	Amarillo-Verde	505/515 nm	1 mL
F8813	0,5 μm	Amarillo-verde	505/515 nm	10 mL
F8820	1,0 μm	Naranja	540/560 nm	10 mL
F8825	2,0 μm	Rojo Nilo	535/575 nm	2 mL
F8827	2,0 μm	Amarillo-verde	505/515 nm	2 mL
F8781 también denominado F-8781	0,02 μm	Azul	365/415 nm	10 mL
F8782 también denominado F-8782	0,02 μm	Carmesí	625/645 nm	2 mL
F8784	0,02 μm	Rojo Nilo	535/575 nm	10 mL
F8787	0,02 μm	Amarillo-verde	505/515 nm	10 mL
F8789 también denominado F-8789	0,04 μm	Rojo oscuro	660/680 nm	1 mL
F8792 también denominado F-8792	0,04 μm	Naranja	540/560 nm	1 mL
F8793	0,04 μm	Rojo	580/605 nm	1 mL
F8794	0,04 μm	Rojo-naranja	565/580 nm	1 mL
F8797	0,1 μm	Azul	350/440 nm	10 mL
F8799	0,1 μm	Infrarrojo	715/755 nm	1 mL
F8800 también denominado F-8800	0,1 μm	Naranja	540/560 nm	10 mL
F8805	0,2 μm	Azul	365/415 nm	10 mL
F8806	0,2 μm	Carmesí	625/645 nm	2 mL
F8809	0,2 μm	Naranja	540/560 nm	10 mL
F8810	0,2 μm	Rojo	580/605 nm	10 mL
F8812	0,5 μm	Rojo	580/605 nm	10 mL
F8814	1,0 μm	Azul	365/415 nm	10 mL
F8815	1,0 μm	Azul	350/440 nm	10 mL
F8819	1,0 μm	Rojo Nilo	535/575 nm	10 mL
F8821	1,0 μm	Rojo	580/605 nm	10 mL
F8824 también denominado F-8824	2,0 μm	Azul	365/415 nm	2 mL
F8826	2,0 μm	Rojo	580/605 nm	2 mL

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Número de catálogo F20881

Precio (CLP)

530.345

Each

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Diámetro (métrico):

0,2 μm

Color:

Naranja

Excitación/emisión:

365/610 nm

Cantidad:

2 mL

Precio (CLP)

530.345

Each

Añadir al carro de la compra

Utilice nuestra amplia selección de microesferas modificadas con carboxilatos FluoSpheres para realizar fácilmente citometría de flujo, microscopía, HTS, HCS, inmunoensayo, así como otras aplicaciones de laboratorio. Los gránulos FluoSphere se pueden utilizar en adsorción pasiva o activa, acoplamiento covalente de proteínas, ácidos nucleicos y biomoléculas para aplicaciones de captura de partículas. Las microesferas FluoSphere están cargadas de colorantes fluorescentes exclusivos, lo que las convierte en las microesferas más brillantes disponibles.

Visualice la fluorescencia más brillante para aplicaciones de laboratorio, como microscopía de fluorescencia, citometría de flujo, HTS, HCS y rastreo celular, gracias a nuestras microesferas FluoSphere modificadas con carboxilatos, fabricadas con microesferas de poliestireno y cargadas con diferentes colorantes patentados. Gracias al uso de métodos de coloración especializados, permite que todas las moléculas de colorante fluorescente estén contenidas dentro de cada microesfera de poliestireno, en lugar de en la superficie. Este entorno de protección dentro del gránulo protege el tinte de efectos medioambientales perjudiciales, como la decoloración fotográfica. Nuestras microesferas modificadas con carboxilato están recubiertas con un polímero hidrofílico que contiene múltiples ácidos carboxílicos para la fijación covalente de los ligandos. Existen diversos tamaños de partículas disponibles para diferentes usos y experimentos de investigación.

For Research Use Only. Not for use in diagnostic procedures.

Especificaciones

Excitación/emisión365/610 nm

Línea de productosFLUOSPHERES

Cantidad2 mL

Modificación de la superficieCarboxilato

ColorNaranja

Diámetro (métrico)0,2 μm

Para utilizar con (aplicación)Microscopía de fluorescencia

MaterialPoliestireno

Tipo de productoMicroesfera modificada con carboxilatos

Unit SizeEach

Contenido y almacenamiento

Almacenar en el refrigerador (2 °C a 8 °C) y proteger de la luz.

Preguntas frecuentes

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When does the warranty guarantee expire for FluoSpheres Carboxylated-Modified Microspheres?

FluoSpheres Carboxylate-Modified Microspheres (Cat. Nos. F8783, F8786, F8801, F8807, F8811, F8803, F8816, F8823, F20881, F10720) have a 1-year warranty guarantee, unless otherwise indicated on the Certificate of Analysis (COA).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the refraction index of FluoSpheres Carboxylate-Modified Microspheres, 0.2 µm, Europium luminescent (365/610), 0.5% solids (Cat. No. F20881)?

We do not measure the refractive index of FluoSpheres Carboxylate-Modified Microspheres, 0.2 µm, Europium luminescent (365/610), 0.5% solids (Cat. No. F20881), but the refractive index of unlabeled polystyrene is 1.591 at 590 nm and that of labeled microspheres is about 1.6.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

Citations & References (3)

Citations & References

Abstract

Integrin beta1-focal adhesion kinase signaling directs the proliferation of metastatic cancer cells disseminated in the lungs.

Authors:Shibue T, Weinberg RA,

Journal:Proc Natl Acad Sci U S A

PubMed ID:19502425

The development of metastases is an extended and inefficient process involving multiple steps. The last of these involves the growth of micrometastases into macroscopic tumors. We show here that intravenously injected, nonmetastatic cancer cells cease proliferating after extravasating into the parenchyma of the lungs; this response is attributable to the ... More

Microfluidic flow-flash: method for investigating protein dynamics.

Authors:Toepke MW, Brewer SH, Vu DM, Rector KD, Morgan JE, Gennis RB, Kenis PJ, Dyer RB

Journal:Anal Chem

PubMed ID:17194129

We report a new method, microfluidic flow-flash, for measuring protein reaction kinetics. The method couples a microscope imaging detection system with a microfluidic flow cell to reduce data acquisition times and sample consumption. This combination allows for the simultaneous collection of spectral and temporal information. The microfluidic flow cell design ... More

Utilization of kinetically enhanced monovalent binding affinity by immunoassays based on multivalent nanoparticle-antibody bioconjugates.

Authors:Soukka T, Härmä H, Paukkunen J, Lövgren T

Journal:Anal Chem

PubMed ID:11393849

The monovalent binding affinity of high binding site density nanoparticle-antibody bioconjugates is shown to exceed the intrinsic affinity of the original, monoclonal antibody. The nanoparticle-antibody bioconjugates were prepared by covalent coupling of antibodies to long-lifetime fluorescent, europium(III) chelate nanoparticles, 107 nm in diameter. Experiments were carried out in standard microtitration ... More

FluoSpheres™ Carboxylate-Modified Microspheres

Preguntas frecuentes

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

I sonicated my 2.0 &micro;m carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

When does the warranty guarantee expire for FluoSpheres Carboxylated-Modified Microspheres?

What is the warranty for FluoSpheres microspheres?

What is the refraction index of FluoSpheres Carboxylate-Modified Microspheres, 0.2 &micro;m, Europium luminescent (365/610), 0.5% solids (Cat. No. F20881)?

Citations & References (3)

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

What is the refraction index of FluoSpheres Carboxylate-Modified Microspheres, 0.2 µm, Europium luminescent (365/610), 0.5% solids (Cat. No. F20881)?