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Citas y referencias (5)
Invitrogen™
Fluorescein Di-β-D-Glucopyranoside (FDGlu)
La di-β-D-glucopiranósido fluoresceína es uno de los sustratos más sensibles para las glucosidasas. El sustrato no fluorescente se hidroliza secuencialmenteMás información
| Número de catálogo | Cantidad |
|---|---|
| F2881 también denominado F-2881 | 5 mg |
Número de catálogo F2881
también denominado F-2881
Precio (CLP)
-
Cantidad:
5 mg
La di-β-D-glucopiranósido fluoresceína es uno de los sustratos más sensibles para las glucosidasas. El sustrato no fluorescente se hidroliza secuencialmente por β-galactosidasa, primero a la monoglucósido fluoresceína y luego a la fluoresceína altamente fluorescente. La hidrólisis mediada por enzimas de la di-β-D-glucopiranósido fluoresceína puede ir seguida del aumento de la absorbancia o de la fluorescencia.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Permeabilidad celularPenetra en la célula
Excitación/emisión490⁄514
Cantidad5 mg
Condiciones de envíoTemperatura ambiente
SustratoSustrato beta-glucosidasa
Método de detecciónFluorescente
Substrate PropertiesSustrato químico
Unit SizeEach
Contenido y almacenamiento
Almacenar en congelador (de -5 a -30 °C).
Citations & References (5)
Citations & References
Abstract
An in situ study of beta-glucosidase activity in normal and Gaucher fibroblasts with fluorogenic probes.
Journal:Cell Biochem Funct
PubMed ID:8403230
'Beta-glucosidase activity was evaluated in situ by means of fluorogenic probes in normal human fibroblasts and fibroblasts from homozygous carriers of the Gaucher trait. Probe internalization, targeting to lysosomes and post-cleavage probe retention were the primary concerns. Internalization and targeting were attempted by in situ photosensitized labilization of lysosomal membranes,
Yeast exo-beta-glucanases can be used as efficient and readily detectable reporter genes in Saccharomyces cerevisiae.
Journal:Yeast
PubMed ID:7975893
'Yeast exo-1,3-beta-glucanases are secretable proteins whose function is basically trophic and may also be involved in cell wall glucan hydrolytic processes. Since fluorescein di(beta-D-glucopyranoside) is a fluorogenic substrate detectable and quantifiable by flow cytometry, it was used for testing the ability of the EXG1 gene product of Saccharomyces cerevisiae and
A flow cytometric assay for lysosomal glucocerebrosidase.
Journal:Anal Biochem
PubMed ID:9177687
'A flow cytometric assay is described for the determination of glucocerebrosidase (GC) activity using fluorescein di-beta-glucopyranoside (FDGlu). Fluorescent product is formed upon intracellular hydrolysis of FDGlu and is measured in the FL1 channel of a flow cytometer. We show that the assay is specific for lysosomal beta-glucosidase or glucocerebrosidase (1)
Directed evolution of a thermophilic beta-glucosidase for cellulosic bioethanol production.
Journal:Appl Biochem Biotechnol
PubMed ID:19834652
Characteristics that would make enzymes more desirable for industrial applications can be improved using directed evolution. We developed a directed evolution technique called random drift mutagenesis (RNDM). Mutant populations are screened and all functional mutants are collected and put forward into the next round of mutagenesis and screening. The goal
Nucleic acid detection using non-radioactive labelling methods.
Journal:Mol Cell Probes
PubMed ID:7477006
Nucleic acid probe-based assays are now widely used in genetic research, human identification, forensics and in a broad spectrum of clinical assays in the fields of microbiology, haematology/oncology and virology. Labelled probes are used in a variety of assay formats including dot-blots, Southern blots (DNA target), Northern blots (RNA target),