Fluorescein Di-β-D-Glucuronide (FDGlcU)
Fluorescein Di-β-D-Glucuronide (FDGlcU)
Citas y referencias (7)
Invitrogen™

Fluorescein Di-β-D-Glucuronide (FDGlcU)

La di-β-D-glucurónido fluoresceína (FDGlcU) es incolora y no fluorescente hasta que se hidroliza al monoglucurónido y luego a la fluoresceínaMás información
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Número de catálogoCantidad
F2915
también denominado F-2915
5 mg
Número de catálogo F2915
también denominado F-2915
Precio (CLP)
-
Cantidad:
5 mg
La di-β-D-glucurónido fluoresceína (FDGlcU) es incolora y no fluorescente hasta que se hidroliza al monoglucurónido y luego a la fluoresceína altamente fluorescente.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Permeabilidad celularPenetra en la célula
Excitación/emisión490⁄514
Cantidad5 mg
Condiciones de envíoTemperatura ambiente
SustratoSustrato beta-glucuronidasa (GUS)
Método de detecciónFluorescente
Substrate PropertiesSustrato químico
Unit SizeEach
Contenido y almacenamiento
Almacenar en congelador (de -5 a -30 °C).

Preguntas frecuentes

Can I make a higher stock solution of Fluorescein Di-β-D-Glucuronide (FDGlcU) (Cat. No. F2915) e.g., 50 mM in PBS?

Unfortunately, we do not have data on the upper limit of solubility for this reagent in PBS. This is something you would need to test empirically.br/>
Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

What is the molecular weight of Fluorescein Di-β-D-Glucuronide (FDGlcU) (Cat. No. F2915)?

The molecular weight of the above product is 684.56 g/mol.br/>
Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

Citations & References (7)

Citations & References
Abstract
Single cell analysis and selection of living retrovirus vector-corrected mucopolysaccharidosis VII cells using a fluorescence-activated cell sorting-based assay for mammalian beta-glucuronidase enzymatic activity.
Authors:Lorincz MC, Parente MK, Roederer M, Nolan GP, Diwu Z, Martin DI, Herzenberg LA, Wolfe JH
Journal:J Biol Chem
PubMed ID:9872999
'Mutations in the acid beta-glucuronidase gene lead to systemic accumulation of undegraded glycosaminoglycans in lysosomes and ultimately to clinical manifestations of mucopolysaccharidosis VII (Sly disease). Gene transfer by retrovirus vectors into murine mucopolysaccharidosis VII hematopoietic stem cells or fibroblasts ameliorates glycosaminoglycan accumulation in some affected tissues. The efficacy of gene ... More
Gene expression imaging by enzymatic catalysis of a fluorescent probe via membrane-anchored beta-glucuronidase.
Authors:Su YC, Chuang KH, Wang YM, Cheng CM, Lin SR, Wang JY, Hwang JJ, Chen BM, Chen KC, Roffler S, Cheng TL,
Journal:Gene Ther
PubMed ID:17235292
'Development of nonimmunogenic and specific reporter genes to monitor gene expression in vivo is important for the optimization of gene therapy protocols. We developed a membrane-anchored form of mouse beta-glucuronidase (mbetaG) as a reporter gene to hydrolyze a nonfluorescent glucuronide probe (fluorescein di-beta-D-glucuronide, (FDGlcU) to a highly fluorescent reporter to ... More
Enzyme-generated intracellular fluorescence for single-cell reporter gene analysis utilizing Escherichia coli beta-glucuronidase.
Authors:Lorincz M, Roederer M, Diwu Z, Herzenberg LA, Nolan GP
Journal:Cytometry
PubMed ID:8866216
'We report the development of a new fluorescence-activated cell sorter (FACS)-based reporter gene system utilizing the enzymatic activity of the E. coli beta-glucuronidase (gus) gene. When loaded with the Gus substrate fluorescein-di-beta-D-glucuronide (FDGlcu), individual mammalian cells expressing and translating gus mRNA liberate sufficient levels of intracellular fluorescein for quantitative analysis ... More
Direct influence of S9 liver homogenate on fluorescence signals: impact on practical applications in a bacterial genotoxicity assay.
Authors:Dreier J, Breitmaier EB, Gocke E, Apfel CM, Page MG
Journal:Mutat Res
PubMed ID:11719102
Assays based on the bacterial SOS-response offer the possibility of automatization of genotoxicity testing for screening of large compound libraries. While existing assays use colorimetric detection or luminescence read-out, we describe here the use of a fluorescence-based system to achieve high sensitivity of detection required for assay miniaturization. Three commonly ... More
Critical elements of oligosaccharide acceptor substrates for the Pasteurella multocida hyaluronan synthase.
Authors:Williams KJ, Halkes KM, Kamerling JP, DeAngelis PL
Journal:J Biol Chem
PubMed ID:16361253
Three-dimensional structures are not available for polysaccharide synthases and only minimal information on the molecular basis for catalysis is known. The Pasteurella multocida hyaluronan synthase (PmHAS) catalyzes the polymerization of the alternating beta1,3-N-acetylglucosamine-beta1,4-glucuronic acid sugar chain by the sequential addition of single monosaccharides to the non-reducing terminus. Therefore, PmHAS possesses ... More
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