FluoSpheres™ Carboxylate-Modified Microspheres

Citas y referencias (4)

Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Name: FluoSpheres™ Carboxylate-Modified Microspheres
SKU: F8792

Consiga la fluorescencia más brillante gracias a las microesferas FluoSphere modificadas con carboxilos, disponibles en diferentes colores y tamaños de partículas.

Cambiar vista

Número de catálogo	Diámetro (métrico)	Color	Excitación/emisión	Cantidad
F8792 también denominado F-8792	0,04 μm	Naranja	540/560 nm	1 mL
F8803	0,1 μm	Amarillo-verde	505/515 nm	10 mL
F8816	1,0 μm	Carmesí	625/645 nm	2 mL
F8823	1,0 μm	Amarillo-verde	505/515 nm	10 mL
F8801	0,1 μm	Rojo	580/605 nm	10 mL
F8811	0,2 μm	Amarillo-verde	505/515 nm	10 mL
F8807	0,2 μm	Rojo oscuro	660/680 nm	2 mL
F10720	0,04 μm	Amarillo-verde, Naranja, Rojo, Rojo oscuro	505/515, 540/560, 580/605, 660/680 nm	1 ml (cada uno)
F20881	0,2 μm	Naranja	365/610 nm	2 mL
F8783 también denominado F-8783	0,02 μm	Rojo oscuro	660/680 nm	2 mL
F8786	0,02 μm	Rojo	580/605 nm	10 mL
F8795 también denominado F-8795	0,04 μm	Amarillo-Verde	505/515 nm	1 mL
F8813	0,5 μm	Amarillo-verde	505/515 nm	10 mL
F8820	1,0 μm	Naranja	540/560 nm	10 mL
F8825	2,0 μm	Rojo Nilo	535/575 nm	2 mL
F8827	2,0 μm	Amarillo-verde	505/515 nm	2 mL
F8781 también denominado F-8781	0,02 μm	Azul	365/415 nm	10 mL
F8782 también denominado F-8782	0,02 μm	Carmesí	625/645 nm	2 mL
F8784	0,02 μm	Rojo Nilo	535/575 nm	10 mL
F8787	0,02 μm	Amarillo-verde	505/515 nm	10 mL
F8789 también denominado F-8789	0,04 μm	Rojo oscuro	660/680 nm	1 mL
F8793	0,04 μm	Rojo	580/605 nm	1 mL
F8794	0,04 μm	Rojo-naranja	565/580 nm	1 mL
F8797	0,1 μm	Azul	350/440 nm	10 mL
F8799	0,1 μm	Infrarrojo	715/755 nm	1 mL
F8800 también denominado F-8800	0,1 μm	Naranja	540/560 nm	10 mL
F8805	0,2 μm	Azul	365/415 nm	10 mL
F8806	0,2 μm	Carmesí	625/645 nm	2 mL
F8809	0,2 μm	Naranja	540/560 nm	10 mL
F8810	0,2 μm	Rojo	580/605 nm	10 mL
F8812	0,5 μm	Rojo	580/605 nm	10 mL
F8814	1,0 μm	Azul	365/415 nm	10 mL
F8815	1,0 μm	Azul	350/440 nm	10 mL
F8819	1,0 μm	Rojo Nilo	535/575 nm	10 mL
F8821	1,0 μm	Rojo	580/605 nm	10 mL
F8824 también denominado F-8824	2,0 μm	Azul	365/415 nm	2 mL
F8826	2,0 μm	Rojo	580/605 nm	2 mL

37 opciones

Número de catálogo F8792

también denominado F-8792

Precio (CLP)

Diámetro (métrico):

0,04 μm

Color:

Naranja

Excitación/emisión:

540/560 nm

Cantidad:

1 mL

Utilice nuestra amplia selección de microesferas modificadas con carboxilatos FluoSpheres para realizar fácilmente citometría de flujo, microscopía, HTS, HCS, inmunoensayo, así como otras aplicaciones de laboratorio. Los gránulos FluoSphere se pueden utilizar en adsorción pasiva o activa, acoplamiento covalente de proteínas, ácidos nucleicos y biomoléculas para aplicaciones de captura de partículas. Las microesferas FluoSphere están cargadas de colorantes fluorescentes exclusivos, lo que las convierte en las microesferas más brillantes disponibles.

Visualice la fluorescencia más brillante para aplicaciones de laboratorio, como microscopía de fluorescencia, citometría de flujo, HTS, HCS y rastreo celular, gracias a nuestras microesferas FluoSphere modificadas con carboxilatos, fabricadas con microesferas de poliestireno y cargadas con diferentes colorantes patentados. Gracias al uso de métodos de coloración especializados, permite que todas las moléculas de colorante fluorescente estén contenidas dentro de cada microesfera de poliestireno, en lugar de en la superficie. Este entorno de protección dentro del gránulo protege el tinte de efectos medioambientales perjudiciales, como la decoloración fotográfica. Nuestras microesferas modificadas con carboxilato están recubiertas con un polímero hidrofílico que contiene múltiples ácidos carboxílicos para la fijación covalente de los ligandos. Existen diversos tamaños de partículas disponibles para diferentes usos y experimentos de investigación.

For Research Use Only. Not for use in diagnostic procedures.

Especificaciones

Excitación/emisión540/560 nm

Línea de productosFLUOSPHERES

Cantidad1 mL

Modificación de la superficieCarboxilato

ColorNaranja

Diámetro (métrico)0,04 μm

Para utilizar con (aplicación)Microscopía de fluorescencia

MaterialPoliestireno

Tipo de productoMicroesfera modificada con carboxilatos

Unit SizeEach

Contenido y almacenamiento

Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

I read that carboxylate-modified latex (CML) beads have a "fluffy coat" of carboxyl groups on their surface. What is meant by this?

The CML beads have a high density of carboxyl groups at the surface. The surface layer is quite hydrophilic and at the appropriate pH (pH>5), are charged; due to electrostatic repulsion, this type of surface is 3-dimensional and may be considered analogous to the fuzz on a tennis ball.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

Citations & References (4)

Citations & References

Abstract

Viral nanoparticles as tools for intravital vascular imaging.

Authors:Lewis JD, Destito G, Zijlstra A, Gonzalez MJ, Quigley JP, Manchester M, Stuhlmann H

Journal:Nat Med

PubMed ID:16501571

'A significant impediment to the widespread use of noninvasive in vivo vascular imaging techniques is the current lack of suitable intravital imaging probes. We describe here a new strategy to use viral nanoparticles as a platform for the multivalent display of fluorescent dyes to image tissues deep inside living organisms. ... More

Angiotensin II attenuates synaptic GABA release and excites paraventricular-rostral ventrolateral medulla output neurons.

Authors:Li DP, Pan HL

Journal:J Pharmacol Exp Ther

PubMed ID:15681656

'The hypothalamic paraventricular nucleus (PVN) neurons regulate sympathetic outflow through projections to the spinal cord and rostral ventrolateral medulla (RVLM). Although the PVN-RVLM pathway is important for the action of brain angiotensin II (Ang II) on autonomic control, the cellular mechanisms involved are not fully known. In this study, we ... More

In situ fluorescent protein imaging with metal film-enhanced total internal reflection microscopy.

Authors:Burghardt TP, Charlesworth JE, Halstead MF, Tarara JE, Ajtai K

Journal:Biophys J

PubMed ID:16565065

Fluorescence detection of single molecules provides a means to investigate protein dynamics minus ambiguities introduced by ensemble averages of unsynchronized protein movement or of protein movement mimicking a local symmetry. For proteins in a biological assembly, taking advantage of the single molecule approach could require single protein isolation from within ... More

Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.

Authors:Mlodzianoski MJ, Curthoys NM, Gunewardene MS, Carter S, Hess ST

Journal:PLoS One

PubMed ID:27002724

'Localization microscopy can image nanoscale cellular details. To address biological questions, the ability to distinguish multiple molecular species simultaneously is invaluable. Here, we present a new version of fluorescence photoactivation localization microscopy (FPALM) which detects the emission spectrum of each localized molecule, and can quantify changes in emission spectrum of ... More

FluoSpheres™ Carboxylate-Modified Microspheres

Preguntas frecuentes

I read that carboxylate-modified latex (CML) beads have a "fluffy coat" of carboxyl groups on their surface. What is meant by this?

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

I sonicated my 2.0 &micro;m carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

What is the warranty for FluoSpheres microspheres?

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Citations & References (4)

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?