FluoSpheres™ Carboxylate-Modified Microspheres
FluoSpheres™ Carboxylate-Modified Microspheres
Citas y referencias (22)
Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Consiga la fluorescencia más brillante gracias a las microesferas FluoSphere modificadas con carboxilos, disponibles en diferentes colores y tamaños de partículas.
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Número de catálogoDiámetro (métrico)ColorExcitación/emisiónCantidad
F88030,1 μmAmarillo-verde505/515 nm10 mL
F88161,0 μmCarmesí625/645 nm2 mL
F88231,0 μmAmarillo-verde505/515 nm10 mL
F88010,1 μmRojo580/605 nm10 mL
F88110,2 μmAmarillo-verde505/515 nm10 mL
F88070,2 μmRojo oscuro660/680 nm2 mL
F107200,04 μmAmarillo-verde, Naranja, Rojo, Rojo oscuro505/515, 540/560, 580/605, 660/680 nm1 ml (cada uno)
F208810,2 μmNaranja365/610 nm2 mL
F8783
también denominado F-8783
0,02 μmRojo oscuro660/680 nm2 mL
F87860,02 μmRojo580/605 nm10 mL
F8795
también denominado F-8795
0,04 μmAmarillo-Verde505/515 nm1 mL
F88130,5 μmAmarillo-verde505/515 nm10 mL
F88201,0 μmNaranja540/560 nm10 mL
F88252,0 μmRojo Nilo535/575 nm2 mL
F88272,0 μmAmarillo-verde505/515 nm2 mL
F8781
también denominado F-8781
0,02 μmAzul365/415 nm10 mL
F8782
también denominado F-8782
0,02 μmCarmesí625/645 nm2 mL
F87840,02 μmRojo Nilo535/575 nm10 mL
F87870,02 μmAmarillo-verde505/515 nm10 mL
F8789
también denominado F-8789
0,04 μmRojo oscuro660/680 nm1 mL
F8792
también denominado F-8792
0,04 μmNaranja540/560 nm1 mL
F87930,04 μmRojo580/605 nm1 mL
F87940,04 μmRojo-naranja565/580 nm1 mL
F87970,1 μmAzul350/440 nm10 mL
F87990,1 μmInfrarrojo715/755 nm1 mL
F8800
también denominado F-8800
0,1 μmNaranja540/560 nm10 mL
F88050,2 μmAzul365/415 nm10 mL
F88060,2 μmCarmesí625/645 nm2 mL
F88090,2 μmNaranja540/560 nm10 mL
F88100,2 μmRojo580/605 nm10 mL
F88120,5 μmRojo580/605 nm10 mL
F88141,0 μmAzul365/415 nm10 mL
F88151,0 μmAzul350/440 nm10 mL
F88191,0 μmRojo Nilo535/575 nm10 mL
F88211,0 μmRojo580/605 nm10 mL
F8824
también denominado F-8824
2,0 μmAzul365/415 nm2 mL
F88262,0 μmRojo580/605 nm2 mL
Número de catálogo F8803
Precio (CLP)
474.258
Each
Añadir al carro de la compra
Diámetro (métrico):
0,1 μm
Color:
Amarillo-verde
Excitación/emisión:
505/515 nm
Cantidad:
10 mL
Precio (CLP)
474.258
Each
Añadir al carro de la compra

Utilice nuestra amplia selección de microesferas modificadas con carboxilatos FluoSpheres para realizar fácilmente citometría de flujo, microscopía, HTS, HCS, inmunoensayo, así como otras aplicaciones de laboratorio. Los gránulos FluoSphere se pueden utilizar en adsorción pasiva o activa, acoplamiento covalente de proteínas, ácidos nucleicos y biomoléculas para aplicaciones de captura de partículas. Las microesferas FluoSphere están cargadas de colorantes fluorescentes exclusivos, lo que las convierte en las microesferas más brillantes disponibles.

Visualice la fluorescencia más brillante para aplicaciones de laboratorio, como microscopía de fluorescencia, citometría de flujo, HTS, HCS y rastreo celular, gracias a nuestras microesferas FluoSphere modificadas con carboxilatos, fabricadas con microesferas de poliestireno y cargadas con diferentes colorantes patentados. Gracias al uso de métodos de coloración especializados, permite que todas las moléculas de colorante fluorescente estén contenidas dentro de cada microesfera de poliestireno, en lugar de en la superficie. Este entorno de protección dentro del gránulo protege el tinte de efectos medioambientales perjudiciales, como la decoloración fotográfica. Nuestras microesferas modificadas con carboxilato están recubiertas con un polímero hidrofílico que contiene múltiples ácidos carboxílicos para la fijación covalente de los ligandos. Existen diversos tamaños de partículas disponibles para diferentes usos y experimentos de investigación.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Excitación/emisión505/515 nm
Línea de productosFLUOSPHERES
Cantidad10 mL
Modificación de la superficieCarboxilato
ColorAmarillo-verde
Diámetro (métrico)0,1 μm
Para utilizar con (aplicación)Microscopía de fluorescencia
MaterialPoliestireno
Tipo de productoMicroesfera modificada con carboxilatos
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When does the warranty guarantee expire for FluoSpheres Carboxylated-Modified Microspheres?

FluoSpheres Carboxylate-Modified Microspheres (Cat. Nos. F8783, F8786, F8801, F8807, F8811, F8803, F8816, F8823, F20881, F10720) have a 1-year warranty guarantee, unless otherwise indicated on the Certificate of Analysis (COA).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

Citations & References (22)

Citations & References
Abstract
Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination.
Authors:Gustafsson MG, Shao L, Carlton PM, Wang CJ, Golubovskaya IN, Cande WZ, Agard DA, Sedat JW,
Journal:Biophys J
PubMed ID:18326650
'Structured illumination microscopy is a method that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit by using spatially structured illumination light. Here we describe how this method can be applied in three dimensions to double the axial as well as the lateral resolution, with true ... More
The power of single and multibeam two-photon microscopy for high-resolution and high-speed deep tissue and intravital imaging.
Authors:Niesner R, Andresen V, Neumann J, Spiecker H, Gunzer M,
Journal:Biophys J
PubMed ID:17557785
'Two-photon microscopy is indispensable for deep tissue and intravital imaging. However, current technology based on single-beam point scanning has reached sensitivity and speed limits because higher performance requires higher laser power leading to sample degradation. We utilize a multifocal scanhead splitting a laser beam into a line of 64 foci, ... More
Role of glycocalyx in leukocyte-endothelial cell adhesion.
Authors:Mulivor AW, Lipowsky HH
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:12234777
'The binding of fluorescently labeled microspheres (FLMs, 0.1-microm diameter) coated with antibody (1a29) to ICAM-1 was studied in postcapillary venules during topical application of the chemoattractant N-formylmethionyl-leucyl-phenylalanine (fMLP). FLM adhesion to endothelial cells (ECs) increased dramatically from 50 to 150 spheres per 100-microm length of venule after superfusion of the ... More
a2 Adrenergic receptor-mediated inhibition of thermogenesis.
Authors:Madden CJ, Tupone D, Cano G, Morrison SF,
Journal:J Neurosci
PubMed ID:23365239
'a2 adrenergic receptor (a2-AR) agonists have been used as antihypertensive agents, in the management of drug withdrawal, and as sedative analgesics. Since a2-AR agonists also influence the regulation of body temperature, we explored their potential as antipyretic agents. This study delineates the central neural substrate for the inhibition of rat ... More
Glucoprivation in the ventrolateral medulla decreases brown adipose tissue sympathetic nerve activity by decreasing the activity of neurons in raphe pallidus.
Authors:Madden CJ,
Journal:Am J Physiol Regul Integr Comp Physiol
PubMed ID:22071154
'In urethane/a-chloralose anesthetized rats, cold exposure increased brown adipose tissue sympathetic nerve activity (BAT SNA: +699 ± 104% control). Intravenous administration of 2-deoxy-D-glucose (2-DG; 200 mg·ml(-1)·kg(-1)) reversed the cold-evoked activation of BAT SNA (nadir: 139 ± 36% of control) and decreased BAT temperature (-1.1 ± 0.2°C), expired CO(2) (-0.4 ± ... More
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