FluoSpheres™ Carboxylate-Modified Microspheres
FluoSpheres™ Carboxylate-Modified Microspheres
Citas y referencias (12)
Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Consiga la fluorescencia más brillante gracias a las microesferas FluoSphere modificadas con carboxilos, disponibles en diferentes colores y tamaños de partículas.
Have Questions?
Cambiar vistabuttonViewtableView
Número de catálogoDiámetro (métrico)ColorExcitación/emisiónCantidad
F88130,5 μmAmarillo-verde505/515 nm10 mL
F88030,1 μmAmarillo-verde505/515 nm10 mL
F88161,0 μmCarmesí625/645 nm2 mL
F88231,0 μmAmarillo-verde505/515 nm10 mL
F88010,1 μmRojo580/605 nm10 mL
F88110,2 μmAmarillo-verde505/515 nm10 mL
F88070,2 μmRojo oscuro660/680 nm2 mL
F107200,04 μmAmarillo-verde, Naranja, Rojo, Rojo oscuro505/515, 540/560, 580/605, 660/680 nm1 ml (cada uno)
F208810,2 μmNaranja365/610 nm2 mL
F8783
también denominado F-8783
0,02 μmRojo oscuro660/680 nm2 mL
F87860,02 μmRojo580/605 nm10 mL
F8795
también denominado F-8795
0,04 μmAmarillo-Verde505/515 nm1 mL
F88201,0 μmNaranja540/560 nm10 mL
F88252,0 μmRojo Nilo535/575 nm2 mL
F88272,0 μmAmarillo-verde505/515 nm2 mL
F8781
también denominado F-8781
0,02 μmAzul365/415 nm10 mL
F8782
también denominado F-8782
0,02 μmCarmesí625/645 nm2 mL
F87840,02 μmRojo Nilo535/575 nm10 mL
F87870,02 μmAmarillo-verde505/515 nm10 mL
F8789
también denominado F-8789
0,04 μmRojo oscuro660/680 nm1 mL
F8792
también denominado F-8792
0,04 μmNaranja540/560 nm1 mL
F87930,04 μmRojo580/605 nm1 mL
F87940,04 μmRojo-naranja565/580 nm1 mL
F87970,1 μmAzul350/440 nm10 mL
F87990,1 μmInfrarrojo715/755 nm1 mL
F8800
también denominado F-8800
0,1 μmNaranja540/560 nm10 mL
F88050,2 μmAzul365/415 nm10 mL
F88060,2 μmCarmesí625/645 nm2 mL
F88090,2 μmNaranja540/560 nm10 mL
F88100,2 μmRojo580/605 nm10 mL
F88120,5 μmRojo580/605 nm10 mL
F88141,0 μmAzul365/415 nm10 mL
F88151,0 μmAzul350/440 nm10 mL
F88191,0 μmRojo Nilo535/575 nm10 mL
F88211,0 μmRojo580/605 nm10 mL
F8824
también denominado F-8824
2,0 μmAzul365/415 nm2 mL
F88262,0 μmRojo580/605 nm2 mL
Número de catálogo F8813
Precio (CLP)
478.218
Each
Añadir al carro de la compra
Diámetro (métrico):
0,5 μm
Color:
Amarillo-verde
Excitación/emisión:
505/515 nm
Cantidad:
10 mL
Precio (CLP)
478.218
Each
Añadir al carro de la compra

Utilice nuestra amplia selección de microesferas modificadas con carboxilatos FluoSpheres para realizar fácilmente citometría de flujo, microscopía, HTS, HCS, inmunoensayo, así como otras aplicaciones de laboratorio. Los gránulos FluoSphere se pueden utilizar en adsorción pasiva o activa, acoplamiento covalente de proteínas, ácidos nucleicos y biomoléculas para aplicaciones de captura de partículas. Las microesferas FluoSphere están cargadas de colorantes fluorescentes exclusivos, lo que las convierte en las microesferas más brillantes disponibles.

Visualice la fluorescencia más brillante para aplicaciones de laboratorio, como microscopía de fluorescencia, citometría de flujo, HTS, HCS y rastreo celular, gracias a nuestras microesferas FluoSphere modificadas con carboxilatos, fabricadas con microesferas de poliestireno y cargadas con diferentes colorantes patentados. Gracias al uso de métodos de coloración especializados, permite que todas las moléculas de colorante fluorescente estén contenidas dentro de cada microesfera de poliestireno, en lugar de en la superficie. Este entorno de protección dentro del gránulo protege el tinte de efectos medioambientales perjudiciales, como la decoloración fotográfica. Nuestras microesferas modificadas con carboxilato están recubiertas con un polímero hidrofílico que contiene múltiples ácidos carboxílicos para la fijación covalente de los ligandos. Existen diversos tamaños de partículas disponibles para diferentes usos y experimentos de investigación.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Excitación/emisión505/515 nm
Línea de productosFLUOSPHERES
Cantidad10 mL
Modificación de la superficieCarboxilato
ColorAmarillo-verde
Diámetro (métrico)0,5 μm
Para utilizar con (aplicación)Microscopía de fluorescencia
MaterialPoliestireno
Tipo de productoMicroesfera modificada con carboxilatos
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Bacterial contamination is the most common cause of microspheres becoming unusable. Many of our particles are supplied with a low level of sodium azide to prevent bacterial contamination, but sometimes this can still occur. Bacterial contamination is best assessed by plating on appropriate growth medium and checking the plates after 72 hr.

Find additional tips, troubleshooting help, and resources within ourMicrospheres Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

Citations & References (12)

Citations & References
Abstract
Altered membrane dynamics of quantum dot-conjugated integrins during osteogenic differentiation of human bone marrow derived progenitor cells.
Authors:Chen H,Titushkin I,Stroscio M,Cho M
Journal:Biophysical journal
PubMed ID:17114225
Functionalized quantum dots offer several advantages for tracking the motion of individual molecules on the cell surface, including selective binding, precise optical identification of cell surface molecules, and detailed examination of the molecular motion without photobleaching. We have used quantum dots conjugated with integrin antibodies and performed studies to quantitatively ... More
Quantitating intracellular transport of polyplexes by spatio-temporal image correlation spectroscopy.
Authors:Kulkarni RP, Wu DD, Davis ME, Fraser SE
Journal:Proc Natl Acad Sci U S A
PubMed ID:15897455
'Quantitatively understanding how nonviral gene delivery vectors (polyplexes) are transported inside cells is essential before they can be optimized for gene therapy and medical applications. In this study, we used spatio-temporal image correlation spectroscopy (ICS) to follow polymer-nucleic acid particles (polyplexes) of various sizes and analyze their diffusive-like and flow ... More
The photon counting histogram in fluorescence fluctuation spectroscopy.
Authors:Chen Y, Müller JD, So PT, Gratton E
Journal:Biophys J
PubMed ID:10388780
'Fluorescence correlation spectroscopy (FCS) is generally used to obtain information about the number of fluorescent particles in a small volume and the diffusion coefficient from the autocorrelation function of the fluorescence signal. Here we demonstrate that photon counting histogram (PCH) analysis constitutes a novel tool for extracting quantities from fluorescence ... More
Thermal fluctuations of grafted microtubules provide evidence of a length-dependent persistence length.
Authors:Pampaloni F, Lattanzi G, Jonás A, Surrey T, Frey E, Florin EL
Journal:Proc Natl Acad Sci U S A
PubMed ID:16801537
'Microtubules are hollow cylindrical structures that constitute one of the three major classes of cytoskeletal filaments. On the mesoscopic length scale of a cell, their material properties are characterized by a single stiffness parameter, the persistence length l(p). Its value, in general, depends on the microscopic interactions between the constituent ... More
Why molecules move along a temperature gradient.
Authors:Duhr S, Braun D
Journal:Proc Natl Acad Sci U S A
PubMed ID:17164337
'Molecules drift along temperature gradients, an effect called thermophoresis, the Soret effect, or thermodiffusion. In liquids, its theoretical foundation is the subject of a long-standing debate. By using an all-optical microfluidic fluorescence method, we present experimental results for DNA and polystyrene beads over a large range of particle sizes, salt ... More
View all FluoSpheres™ Carboxylate-Modified Microspheres citations