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Citas y referencias (28)
Invitrogen™
Guanosine 5'-O-(3-Thiotriphosphate), BODIPY™ FL Thioester, Sodium Salt (BODIPY™ FL GTP-γ-S, Thioester)
GTP-γ-S BODIPY FL se une a proteínas de unión a GTP. Para crear este nucleótido fluorescente, el fluoróforo BODIPY FLMás información
| Número de catálogo | Cantidad |
|---|---|
| G22183 también denominado G-22183 | 50 μl |
Número de catálogo G22183
también denominado G-22183
Precio (CLP)
-
Cantidad:
50 μl
GTP-γ-S BODIPY FL se une a proteínas de unión a GTP. Para crear este nucleótido fluorescente, el fluoróforo BODIPY FL se vinculó a través de γ-tiol de GTP-γ-S. La fluorescencia de BODIPY FL GTP-γ-S se desactiva un ∼90 % en relación con la del colorante libre, pero puede recuperarse al unirse a las proteínas G. Además de su uso potencial para estudios de unión, el tioéter GTP-γ-S BODIPY FL es un nuevo sustrato importante para Fhit, un miembro de la superfamilia de proteínas de unión a nucleótidos de la tríada de histidina.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de etiquetadoMarcaje químico, marcaje directo
Etiqueta o tinteBODIPY™ FL
Tipo de productoGuanosina 5'-O
Cantidad50 μl
Condiciones de envíoHielo húmedo
Concentración5 mM
Línea de productosBODIPY
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Citations & References (28)
Citations & References
Abstract
Detection of G proteins by affinity probe capillary electrophoresis using a fluorescently labeled GTP analogue.
Journal:Anal Chem
PubMed ID:14632149
'An affinity probe capillary electrophoresis (APCE) assay for guanine-nucleotide-binding proteins (G proteins) was developed using BODIPY FL GTPgammaS (BGTPgammaS), a fluorescently labeled GTP analogue, as the affinity probe. In the assay, BGTPgammaS was incubated with samples containing G proteins and the resulting mixtures of BGTPgammaS-G protein complexes and free BGTPgammaS
Designed FHIT alleles establish that Fhit-induced apoptosis in cancer cells is limited by substrate binding.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12574506
'The FHIT gene is inactivated early in the development of many human tumors, and Fhit-deficient mice have increased cancer incidence. Viral reexpression of Fhit kills Fhit-deficient cells by induction of apoptosis. Fhit, a member of branch 2 of the histidine-triad superfamily of nucleoside monophosphate hydrolases and transferases, is a diadenosine
Fhit-nucleotide specificity probed with novel fluorescent and fluorogenic substrates.
Journal:J Biol Chem
PubMed ID:10671479
'Fhit, a member of the histidine triad superfamily of nucleotide-binding proteins, binds and cleaves diadenosine polyphosphates and functions as a tumor suppressor in human epithelial cancers. Function of Fhit in tumor suppression does not require diadenosine polyphosphate cleavage but correlates with the ability to form substrate complexes. As diadenosine polyphosphates
Mechanism of regulation of prokaryotic tubulin-like GTPase FtsZ by membrane protein EzrA.
Journal:J Biol Chem
PubMed ID:17043359
'At initiation of cell division, FtsZ, a tubulin-like GTPase, assembles into a so-called Z-ring structure at the site of division. The formation of Z ring is negatively regulated by EzrA, which ensures only one ring at the midcell per cell cycle. The mechanism leading to the negative regulation of Z-ring
Use of a native affinity ligand for the detection of G proteins by capillary isoelectric focusing with laser-induced fluorescence detection.
Journal:Electrophoresis
PubMed ID:15274014
'Affinity probe capillary isoelectric focusing (CIEF) with laser-induced fluorescence was explored for detection of Ras-like G proteins. In the assay, a fluorescent BODIPY FL GTP analogue (BGTPgammaS) and G protein were incubated resulting in formation of BGTPgammaS-G protein complex. Excess BGTPgammaS was separated from BGTPgammaS-G protein complex by CIEF using