Tinción de lípidos neutros HCS LipidTOX™ Red, para adquisición de imágenes celulares

Citas y referencias (16)

Invitrogen™

Tinción de lípidos neutros HCS LipidTOX™ Red, para adquisición de imágenes celulares

La acumulación intracelular de lípidos neutros, esteatosis, se desencadena a menudo por medicamentos que afectan al metabolismo de los ácidosMás información

Número de catálogo	Cantidad
H34476	1 unidad

Número de catálogo H34476

Precio (CLP)

613.963

Añadir al carro de la compra

Cantidad:

1 unidad

Precio (CLP)

613.963

Añadir al carro de la compra

La acumulación intracelular de lípidos neutros, esteatosis, se desencadena a menudo por medicamentos que afectan al metabolismo de los ácidos grasos o lípidos neutros. El tinte de lípidos neutros HCS LipidTOX™ Red se desarrolló para caracterizar los efectos potencialmente tóxicos de compuestos sobre el metabolismo lipídico en líneas celulares de mamíferos. El tinte de lípidos neutros LipidTOX™ tiene una afinidad extraordinariamente alta para las microgotas de lípidos neutros y se puede detectar por microscopía de fluorescencia o un lector de HCS. Esta sonda es compatible con los reactivos de detección de fosfolípidos (H34350, H34351) LipidTOX™ HCS. Los tintes de lípidos neutros LipidTOX™ HCS también pueden utilizarse para supervisar la formación y la diferenciación de los adipocitos, un proceso llamado adipogénesis.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Método de detecciónFluorescente

Para utilizar con (equipo)Instrumentos de alto contenido

Línea de productosLipidTOX

Cantidad1 unidad

Condiciones de envíoTemperatura ambiente

Tipo de etiquetaOtras etiquetas o colorantes

Tipo de productoTinción lipídica

SubCellular LocalizationCell Membranes and Lipids , Lipids

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

What kind of filter sets can I use with HCS LipidTOX neutral lipid stains?

LipidTOX Green neutral lipid stain can be imaged with filter sets appropriate for Alexa Fluor 488 dye or fluorescein. LipidTOX Red neutral lipid stain is best imaged with filter sets appropriate for Alexa Fluor 594 dye or Texas Red dye. LipidTOX Deep Red neutral lipid stain can imaged with filter sets appropriate for Alexa Fluor 647 dye or Cy5 dye.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the plasma membrane of my cells, but there are several dyes to choose from. Which one should I use?

For live-cell imaging, the CellVue and CellMask Plasma Membrane Stains are the most uniform and the slowest to be endocytosed. However, they are not the best choice if you wish to fix and permeabilize your cells, such as for antibody labeling. Wheat germ agglutinin (WGA) conjugates are also able to label live cells, or can label already formaldehyde-fixed cells. They can survive subsequent permeabilization with detergents, such as Triton X-100. If cells are already permeabilized, WGA will label internal structures as well. Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods. Following page will help you choose (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (16)

Citations & References

Abstract

Vascular lipid accumulation, lipoprotein oxidation, and macrophage lipid uptake in hypercholesterolemic zebrafish.

Authors:Stoletov K, Fang L, Choi SH, Hartvigsen K, Hansen LF, Hall C, Pattison J, Juliano J, Miller ER, Almazan F, Crosier P, Witztum JL, Klemke RL, Miller YI,

Journal:Circ Res

PubMed ID:19265037

'Lipid accumulation in arteries induces vascular inflammation and atherosclerosis, the major cause of heart attack and stroke in humans. Extreme hyperlipidemia induced in mice and rabbits enables modeling many aspects of human atherosclerosis, but microscopic examination of plaques is possible only postmortem. Here we report that feeding adult zebrafish (Danio ... More

Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production.

Authors:Brennan L, Blanco Fernández A, Mostaert AS, Owende P,

Journal:J Microbiol Methods

PubMed ID:22521923

'This paper describes a microalgal cell lipid fluorescence enhancement method using BODIPY(505/515), which can be used to screen for lipids in wild-type microalgae and to monitor lipid content within microalgae production processes to determine optimal harvesting time. The study was based on four microalgae species (Dunaliella teteriolecta, Tetraselmis suecica, Nannochloropsis ... More

Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains.

Authors:Work VH, Radakovits R, Jinkerson RE, Meuser JE, Elliott LG, Vinyard DJ, Laurens LM, Dismukes GC, Posewitz MC,

Journal:Eukaryot Cell

PubMed ID:20562225

'The accumulation of bioenergy carriers was assessed in two starchless mutants of Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7-10 [isoamylase] mutants), a control strain (CC124), and two complemented strains of the sta7-10 mutant. The results indicate that the genetic blockage of starch synthesis in the sta6 and sta7-10 mutants ... More

Knockdown of ACAT-1 reduces amyloidogenic processing of APP.

Authors:Huttunen HJ, Greco C, Kovacs DM

Journal:FEBS Lett

PubMed ID:17412327

'Previous studies have shown that acyl-coenzyme A:cholesterol acyl transferase (ACAT), an enzyme that controls cellular equilibrium between free cholesterol and cholesteryl esters, modulates proteolytic processing of APP in cell-based and animal models of Alzheimer''s disease. Here we report that ACAT-1 RNAi reduced cellular ACAT-1 protein by approximately 50% and cholesteryl ... More

Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae.

Authors:Pereira H, Barreira L, Mozes A, Florindo C, Polo C, Duarte CV, Custódio L, Varela J,

Journal:Biotechnol Biofuels

PubMed ID:22192119

We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the ... More

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