Tinción de lípidos neutros HCS LipidTOX™ Deep Red, para adquisición de imágenes celulares

Citas y referencias (18)

Invitrogen™

Tinción de lípidos neutros HCS LipidTOX™ Deep Red, para adquisición de imágenes celulares

La acumulación intracelular de lípidos neutros, esteatosis, se desencadena a menudo por medicamentos que afectan al metabolismo de los ácidosMás información

Número de catálogo	Cantidad
H34477	1 unidad

Número de catálogo H34477

Precio (CLP)

611.672

Añadir al carro de la compra

Cantidad:

1 unidad

Precio (CLP)

611.672

Añadir al carro de la compra

La acumulación intracelular de lípidos neutros, esteatosis, se desencadena a menudo por medicamentos que afectan al metabolismo de los ácidos grasos o lípidos neutros. El tinte de lípidos neutros HCS LipidTOX™ rojo oscuro se desarrolló para caracterizar los efectos potencialmente tóxicos de compuestos sobre el metabolismo lipídico en líneas celulares de mamíferos. El tinte de lípidos neutros LipidTOX™ tiene una afinidad extraordinariamente alta para las microgotas de lípidos neutros y se puede detectar por microscopía de fluorescencia o un lector de HCS. Esta sonda es compatible con los reactivos de detección de fosfolípidos (H34350, H34351) LipidTOX™ HCS. Los tintes de lípidos neutros LipidTOX™ HCS también pueden utilizarse para supervisar la formación y la diferenciación de los adipocitos, un proceso llamado adipogénesis.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorRojo oscuro

Método de detecciónFluorescente

Para utilizar con (equipo)Instrumentos de alto contenido

Línea de productosLipidTOX

Cantidad1 unidad

Condiciones de envíoTemperatura ambiente

Tipo de etiquetaFluorescent Dye

Tipo de productoTinción lipídica

SubCellular LocalizationMembranas celulares y lípidos, Lipids

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

What kind of filter sets can I use with HCS LipidTOX neutral lipid stains?

LipidTOX Green neutral lipid stain can be imaged with filter sets appropriate for Alexa Fluor 488 dye or fluorescein. LipidTOX Red neutral lipid stain is best imaged with filter sets appropriate for Alexa Fluor 594 dye or Texas Red dye. LipidTOX Deep Red neutral lipid stain can imaged with filter sets appropriate for Alexa Fluor 647 dye or Cy5 dye.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the HCS LipidTOX Deep Red Neutral Lipid Stain (Cat. No. H34477) ready to use, or do I need to dilute it in 1X PBS or DMSO?

The HCS LipidTOX Deep Red Neutral Lipid Stain is provided as a solution in DMSO and needs to be diluted before use. The recommended dilution is 1:200 in 1X PBS or other buffer (e.g. HBSS, TBS, etc).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What buffer should I use with HCS LipidTOX™ Deep Red Neutral Lipid Stain, for cellular imaging (Cat. No. H3447)?

The ideal buffer with HCS LipidTOX Deep Red Neutral Lipid Stain is PBS at pH 7.4.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the plasma membrane of my cells, but there are several dyes to choose from. Which one should I use?

For live-cell imaging, the CellVue and CellMask Plasma Membrane Stains are the most uniform and the slowest to be endocytosed. However, they are not the best choice if you wish to fix and permeabilize your cells, such as for antibody labeling. Wheat germ agglutinin (WGA) conjugates are also able to label live cells, or can label already formaldehyde-fixed cells. They can survive subsequent permeabilization with detergents, such as Triton X-100. If cells are already permeabilized, WGA will label internal structures as well. Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods. Following page will help you choose (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (18)

Citations & References

Abstract

Functional analysis of FSP27 protein regions for lipid droplet localization, caspase-dependent apoptosis, and dimerization with CIDEA.

Authors:Liu K, Zhou S, Kim JY, Tillison K, Majors D, Rearick D, Lee JH, Fernandez-Boyanapalli RF, Barricklow K, Houston MS, Smas CM,

Journal:Am J Physiol Endocrinol Metab

PubMed ID:19843876

'The adipocyte-specific protein FSP27, also known as CIDEC, is one of three cell death-inducing DFF45-like effector (CIDE) proteins. The first known function for CIDEs was promotion of apoptosis upon ectopic expression in mammalian cells. Recent studies in endogenous settings demonstrated key roles for CIDEs in energy metabolism. FSP27 is a ... More

Functional Interactions between Mldp (LSDP5) and Abhd5 in the Control of Intracellular Lipid Accumulation.

Authors:Granneman JG, Moore HP, Mottillo EP, Zhu Z,

Journal:J Biol Chem

PubMed ID:19064991

'Cellular lipid metabolism is regulated in part by protein-protein interactions near the surface of intracellular lipid droplets. This work investigated functional interactions between Abhd5, a protein activator of the lipase Atgl, and Mldp, a lipid droplet scaffold protein that is highly expressed in oxidative tissues. Abhd5 was highly targeted to ... More

Fluorescent high-content imaging allows the discrimination and quantitation of E-LDL-induced lipid droplets and Ox-LDL-generated phospholipidosis in human macrophages.

Authors:Grandl M, Schmitz G,

Journal:Cytometry A

PubMed ID:20014301

'Macrophage foam cells formed during uptake of atherogenic lipoproteins are a hallmark of atherosclerotic lesion development. In this study, human macrophages were incubated with two prototypic atherogenic LDL modifications enzymatically degraded LDL (E-LDL) and oxidized LDL (Ox-LDL) prepared from the same donor LDL. To detect differences in macrophage lipid storage, ... More

Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production.

Authors:Brennan L, Blanco Fernández A, Mostaert AS, Owende P,

Journal:J Microbiol Methods

PubMed ID:22521923

'This paper describes a microalgal cell lipid fluorescence enhancement method using BODIPY(505/515), which can be used to screen for lipids in wild-type microalgae and to monitor lipid content within microalgae production processes to determine optimal harvesting time. The study was based on four microalgae species (Dunaliella teteriolecta, Tetraselmis suecica, Nannochloropsis ... More

Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains.

Authors:Work VH, Radakovits R, Jinkerson RE, Meuser JE, Elliott LG, Vinyard DJ, Laurens LM, Dismukes GC, Posewitz MC,

Journal:Eukaryot Cell

PubMed ID:20562225

'The accumulation of bioenergy carriers was assessed in two starchless mutants of Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7-10 [isoamylase] mutants), a control strain (CC124), and two complemented strains of the sta7-10 mutant. The results indicate that the genetic blockage of starch synthesis in the sta6 and sta7-10 mutants ... More

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