Células estrelladas hepáticas miofibroblásticas humanas activadas (MF-HSC)

Las células hepáticas miofibroblásticas humanas y activadas Gibco con forma de estrella (MF-HSC) se producen a partir de células humanasMás información

Número de catálogo	Cantidad
HMFHSC	1 vial

Número de catálogo HMFHSC

Precio (CLP)

Cantidad:

1 vial

Las células hepáticas miofibroblásticas humanas y activadas Gibco con forma de estrella (MF-HSC) se producen a partir de células humanas quiescentes aisladas con forma de estrella (q- HSC). Las células se obtienen de un solo donante y se suministran en formato crioconservado idéntico a los hepatocitos humanos plateables de un solo donante.

Cada vial contiene un mínimo de un millón de células viables en pase cinco, suficientes para al menos cuatro placas de 96 pocillos en cultivo 2D.

Protocolo simplificado
Los MF-HSC activados mediante protocolos simplificados están indicados para construir cultivos hepáticos simultáneos con hepatocitos primarios para estudiar modelos de enfermedades in vitro como la fibrosis hepática. Las células crioconservadas pueden descongelarse y cultivadas a su conveniencia en tan solo 30 minutos. Pruébelo por su cuenta, o solicite una demostración con un científico experimentado.

Control de calidad exhaustivo
Cada lote se somete a pruebas para:
• Viabilidad > 75 %
• Eficacia de los accesorios
• Morfología apropiada
• Pureza basada en actina α de músculo liso (α-SMA)
• Activación en respuesta al factor de crecimiento transformador-β1 (TGF-β1)

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de productoCélulas estrelladas hepáticas miofibroblásticas (MF-HSC)

Cantidad1 vial

Condiciones de envíoContenedor de transporte en seco

Fuente de dadoresDonante único

Especie del huéspedHumano

Forma físicaCrioconservado

Unit SizeEach

Contenido y almacenamiento

>1 millones de células viables/vial

Preguntas frecuentes

Who should I contact if I am looking for hepatocytes with particular characteristics?

Our hepatocytes are prequalified and sold according to application, such as induction, short-term metabolism, and transporter uptake. If you require hepatocytes with particular P450 values or donor specifications, contact our Hepatic Biology Tech Support Team (hepaticproducts@thermofisher.com.com) or (866) 952-3559 to help with specific lot recommendations.

Who can I call if I have technical questions about hepatocyte cell culture?

Thermo Fisher Scientific has a dedicated team to answer questions regarding hepatocyte cell culture. You can send a question by email (hepaticproducts@thermofisher.com) or contact us by phone: US toll-free: (866) 952-3559

Are there guidelines for the procurement of human liver tissue?

Thermofisher Scientific works with a variety of human tissue sources, including Tissue and Organ Procurement Organizations, qualified Research Tissue Organizations and prominent academic and medical centers through collaborations that follow rigorous regulations, certifications and/or accreditations (“Source Facilities”). Tissues obtained through source facilities are consistent with the applicable legal and ethical practices of the United States.

Source Facilities are required by federal law to maintain accurate records and donor confidentiality as well as adhere to certain applicable laws governing tissue used for research globally. These include, but are not limited to:

- 45 CFR Part 46, subparts A, B, C, D where applicable (US federal guidance on the use of human subjects in research)
- Health Insurance Portability and Accountability Act (HIPAA) (addressing issues of confidentiality of personal information)
- National Organ Transplant Act (42 CFR Part 482)
- The Uniform Anatomical Gift Act (UAGA) of 1968, revised 1984

Where do you get your human liver tissue?

The human liver cells are derived from tissue obtained from accredited institutions. Consent is obtained by these institutions from the donor or the donor’s legal next of kin, for the use of the tissue and its derivatives for research purpose.

How many times do you passage quiescent human stellate cells (q-HSCs)?

The qHSCs only go through a few passages, just sufficient for them to get activated. However, the low number of passages maintains their ability to get induced by inflammatory cytokines.

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