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Citas y referencias (32)
Invitrogen™
ImaGene Green™ C12FDG lacZ Gene Expression Kit
El kit de expresión de genes lacZ ImaGene Green™ contiene un sustrato de galactosidasa a base de fluoresceína que seMás información
| Número de catálogo | Cantidad |
|---|---|
| I2904 | 1 kit |
Número de catálogo I2904
Precio (CLP)
840.762
Each
Cantidad:
1 kit
Precio (CLP)
840.762
Each
El kit de expresión de genes lacZ ImaGene Green™ contiene un sustrato de galactosidasa a base de fluoresceína que se ha modificado covalentemente para incluir una fracción lipofílica de 12 carbonos, C12FDG. Una vez dentro de la célula, los sustratos son escindidos por la β-galactosidasa, generando un producto fluorescente que es bien retenido por las células, probablemente por la incorporación de la cola lipofílica dentro de la membrana celular. Además de la C12FDG, los kits también incluyen el inhibidor de la β-galactosidasa de amplio espectro, PETG, y difosfato de cloroquina para inhibir la hidrólisis ácida del sustrato.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ensayoEnsayo de β-Gal (lacZ)
Método de detecciónFluorescente
Línea de productosImaGene Green
Tipo de productoSistema de ensayo de gen indicador de β-galactosidasa
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
SustratoC12FDG
Propiedades de sustratoSustrato químico
Tipo de sustratoSustrato Beta-Gal
Enzima dianaBeta-galactosidasa
FormatoKit
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.
Citations & References (32)
Citations & References
Abstract
Rapid flow cytometric method for measuring senescence associated beta-galactosidase activity in human fibroblasts.
Journal:Cytometry A
PubMed ID:19777541
'Senescence associated-beta-galactosidase (SA-beta-gal) activity is a widely used marker for cellular senenescence. SA-beta-gal activity is routinely detected cytochemically, manually discriminating negative from positive cells. This method is time-consuming, subjective and therefore prone to operator-error. We aimed to optimize a flow cytometric method described by other workers using endothelial cells to
Optical imaging fiber-based single live cell arrays: a high-density cell assay platform.
Journal:Anal Chem
PubMed ID:12141663
'A high-density, ordered array containing thousands of microwells is fabricated on an optical imaging fiber. Each individually addressable microwell is used to accommodate a single living cell. A charged coupled device (CCD) detector is employed to monitor and spatially resolve the fluorescence signals obtained from each individual cell, allowing simultaneous
An optimized electroporation protocol applicable to a wide range of cell lines.
Journal:Biotechniques
PubMed ID:7873174
'A number of transfection methods for mammalian cells are available; however, many cell lines may appear resistant to efficient transfection, or at best, necessitate lengthy optimization procedures in recommended protocols. We describe here an electroporation protocol that yields highly efficient gene transfer (20%-100% of surviving cells) in all 19 cell
Mapping mechanisms and charting the time course of premature cell senescence and apoptosis: lysosomal dysfunction and ganglioside accumulation in endothelial cells.
Journal:Am J Physiol Renal Physiol
PubMed ID:17928415
'Endothelial cells subjected to glycated collagen I develop premature senescence within 3-5 days, as revealed by increased senescence-associated beta-galactosidase activity, decreased proliferation, and an increase in cell size. Here, we analyzed the time course and possible mechanisms of this process. Lysosomal integrity studies revealed a rapid collapse of pH gradient
Use of fluorescence-activated cell sorting for rapid isolation of insect cells harboring recombinant baculovirus.
Journal:Methods Cell Biol
PubMed ID:7877509