NovaFluor™ Antibody Conjugation Kits

Name: NovaFluor™ Antibody Conjugation Kits
SKU: K06T04L019

Saque el máximo partido al diseño del panel con anticuerpos conjugados NovaFluors: los tintes se han diseñado específicamente para que los espectros de emisión sean estrechos y la excitación láser cruzada sea mínima.

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Número de catálogo	Etiqueta o tinte	Tipo de producto	Emisión	Cantidad
K06T04L019	NovaFluor™ Red 700	Kit de conjugado	700 nm	100 μg
K06T04L008	NovaFluor™ Blue 510	Kit de conjugado	510 nm	100 μg
K06T04L002	NovaFluor™ Blue 530	Kit de conjugado	530 nm	100 μg
K06T04L009	NovaFluor™ Blue 555	Kit de conjugado	555 nm	100 μg
K06T04L010	NovaFluor™ Blue 585	Kit de conjugado	585 nm	100 μg
K06T04L003	NovaFluor™ Blue 610-30S	Kit de conjugado	610 nm	100 μg
K06T04L011	NovaFluor™ Blue 610-70S	Kit de conjugado	610 nm	100 μg
K06T04L004	NovaFluor™ Blue 660-40S	Kit de conjugado	660 nm	100 μg
K06T04L021	NovaFluor™ Blue 690	Kit de conjugado	690 nm	100 μg
K06T04L012	NovaFluor™ Blue 660-120S	Kit de conjugado	660 nm	100 μg
K06T04L026	NovaFluor™ Blue 760	Kit de conjugado	760 nm	100 μg
K06T04L017	NovaFluor™ Red 660	Kit de conjugado	660 nm	100 μg
K06T04L018	NovaFluor™ Red 685	Kit de conjugado	685 nm	100 μg
K06T04L020	NovaFluor™ Red 710	Kit de conjugado	710 nm	100 μg
K06T04L023	NovaFluor™ Red 725	Kit de conjugado	725 nm	100 μg
K06T04L024	NovaFluor™ Red 755	Kit de conjugado	755 nm	100 μg
K06T04L005	NovaFluor™ Yellow 570	Kit de conjugado	570 nm	100 μg
K06T04L013	NovaFluor™ Yellow 590	Kit de conjugado	590 nm	100 μg
K06T04L006	NovaFluor™ Yellow 610	Kit de conjugado	610 nm	100 μg
K06T04L007	NovaFluor™ Yellow 660	Kit de conjugado	660 nm	100 μg
K06T04L014	NovaFluor™ Yellow 690	Kit de conjugado	690 nm	100 μg
K06T04L015	NovaFluor™ Yellow 700	Kit de conjugado	700 nm	100 μg
K06T04L016	NovaFluor™ Yellow 730	Kit de conjugado	730 nm	100 μg
K06T04L022	NovaFluor™ Yellow 755	Kit de conjugado	755 nm	100 μg
K06T04L034	NovaFluor™ Blue 745	Conjugation Kit	748	100 μg
K06T04L035	NovaFluor™ Red 800	Conjugation Kit	799	100 μg

26 opciones

Número de catálogo K06T04L019

Precio (CLP)

Etiqueta o tinte:

NovaFluor™ Red 700

Tipo de producto:

Kit de conjugado

Emisión:

700 nm

Cantidad:

100 μg

Los kits de conjugación NovaFluor proporcionan un flujo de trabajo simple para conjugar un fluoróforo excitable azul, amarillo-verde o rojo con el anticuerpo de su elección en solo 3 sencillos pasos. La plataforma Phiton de fluorescencia por diseño permite agregar parámetros a sus paneles actuales en la investigación de descubrimiento y resuelve las poblaciones teñidas con una excitación cruzada cercana a cero, con una falta de emisión en los canales violetas que afectan a otros fluoróforos.

NovaFluor Conjugation Kit provides features including:

A clean and bright antibody label to increase the number of parameters on conventional and spectral flow cytometers
Resolve populations with limited/near-zero cross excitation and the lowest spread performance of any blue, yellow-green or red excited fluorescent labels
Simple 3-step protocol, with 60 sec. of hands-on time for conjugating 10–100 μg of antibody per label
Up to 70% less excitation when compared to PE tandems, resulting in decreased spillover and higher resolution

3-step conjugation method

Attach NovaFluor Linker to antibody of choice
Remove excess NovaFluor Linker from DNA-conjugate antibody
Anneal NovaFluor to oligo-conjugated antibody

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Emisión700 nm

Para utilizar con (aplicación)Citometría de flujo

Etiqueta o tinteNovaFluor™ Red 700

Tipo de productoKit de conjugado

Cantidad100 μg

Línea de productosNovaFluor

Unit SizeEach

Preguntas frecuentes

What is the NovaFluor dye structure and how does this impact the NovaFluor antibody conjugation workflow?

NovaFluor dyes are based on Phiton technology utilizing novel nucleic acid dye structures. The dyes work with FRET technology and have a clear benefit versus tandem dyes, as the structures are extremely stable. More information can be found on our NovaFluor website.

Due to the nucleotide structure, some adherence of the NovaFluor dyes to dead cells is expected. Good viability of the sample and inclusion of a fixable viability stain is recommended. Using nucleotide-staining viability dyes such as DAPI, PI, and 7-AAD dyes is not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use NovaFluor antibody conjugation kits for conjugation of antibodies targeting intracellular epitopes?

NovaFluor antibody conjugation kits can be used for conjugation of antibodies targeting intracellular epitopes. The labeling needs to be performed with 10 µL CellBlox Plus Blocking Buffer (Cat. No. C001T02F01, C001T03F01, C001T06F01) to block non-specific binding of NovaFluor labels with cells, after fixation and permeabilization.

If NovaFluor dyes are used for surface targets as well as intracellular targets, first add 5 µL CellBlox Plus Blocking Buffer during staining with the surface markers. Then, after completing the staining, washing, fixation, and permeabilization, add 10 µL CellBlox Plus Blocking Buffer again to block non-specific intracellular binding while labeling with the NovaFluor conjugates for intracellular targets.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I label my antibody with more than one NovaFluor label if I purchased more than one NovaFluor conjugation kit?

Yes, you can. After performing the steps on Days 1 and 2, your Antibody-NovaFluor Linker conjugate can be attached to the NovaFluor label at whatever volume you like, as long as the ratio of the Antibody-NovaFluor Linker to the NovaFluor label is kept constant.

Example: If you purchased two NovaFluor conjugation kits, one with NovaFluor Blue 610-30S and one with NovaFluor Yellow 610, use the contents of both kits to modify your two antibodies of interest (both 100 µg), completing all steps outlined for Days 1 and 2 with no modification. After resuspending your antibodies into PBS and after the second round of ammonium sulfate precipitation, you should have 100 µL of each Antibody-NovaFluor Linker conjugate. You can now divide the antibody solutions in half (50 µL each) and add half of each NovaFluor label to the divided antibody solutions (150 µL of NovaFluor label to each 50 µL of Antibody-NovaFluor Linker), giving you 200 µL total of each antibody conjugated to both NovaFluor Blue 610-30S and NovaFluor Yellow 610. After mixing, continue with the protocol and incubate overnight at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I store NovaFluor-antibody conjugates?

We recommend storing new NovaFluor-antibody conjugates at 2-8 degrees C, protected from light. If desired, NovaFluor-antibody conjugates can be stored with 0.09% sodium azide. DO NOT store NovaFluor-antibody conjugates at -80 degrees C or -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For the NovaFluor conjugation kits, in Step 16 of the procedure, can you provide more information about how I should orient my tube and describe the appearance of the pellet following precipitation?

We have included Figure 4 in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025061_NovaFluorConjugationKit_UG.pdf) to show how the pellet appears following the precipitation step. Although the microcentrifuge tube can be centrifuged in any orientation, we recommend orienting it in the same direction for both centrifugation steps so that your pellet is in the same place (Figure 3). The pellet is often smaller after the second precipitation, so it is helpful to know where to expect it.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all NovaFluor™ Antibody Conjugation Kits FAQs