Luminaris Probe qPCR Master Mix, low ROX

Name: Luminaris Probe qPCR Master Mix, low ROX
SKU: K0941

Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-stepMás información

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Número de catálogo	N.º de reacciones
K0941	250 reacciones
K0942	500 reacciones
K0943	1250 reacciones
K0944	5000 reacciones

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Número de catálogo K0941

Precio (CLP)

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N.º de reacciones:

250 reacciones

Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• UDG in the master mix to prevent carry-over contamination• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Probe Low ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Fidelidad (frente a Taq)2 X

Para utilizar con (equipo)Sistema 7500, QuantStudio™, Sistema ViiA™ 7, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P

N.º de reacciones250 reacciones

PolimerasaADN polimerasa Taq

Línea de productosLuminaris

Tipo de productoqPCR Master Mix

Cantidad250 reactions

Tipo de muestraADN, ADNbc

Suficiente para250 reacciones

Concentración2X

Método de detecciónSonda de cebado

Para utilizar con (aplicación)Gene Expression

GC-Rich PCR PerformanceHigh

Método de PCRqPCR

Velocidad de reacciónEstándar

Unit SizeEach

Contenido y almacenamiento

Contains:
• 2 x 1.25 mL Luminaris Probe Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer; sufficient for 250 x 20 μL reactions. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms.
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.

Preguntas frecuentes

Does the yellow dye from the Yellow Sample Buffer provided with Luminaris Color and DyNAmo Flash qPCR Master Mixes influence qPCR performance?

The addition of yellow dye from the Yellow Sample Buffer to qPCR reaction has no influence on the efficiency, specificity, sensitivity or Cq values. Using the Yellow Sample Buffer is optional, but the dye helps to see in which wells the sample has been already added.

Does dUTP in Luminaris qPCR master mix influence qPCR efficiency?

Luminaris qPCR master mixes have all dTTP substituted with dUTP. dUTP presence does not influence the reaction speed and efficiency of the master mix. 100% substitution of dTTP with dUTP facilitates an efficient clearance of the contamination from previous PCR reactions.

What enzyme is used in Luminaris qPCR Master Mixes?

Luminaris qPCR master mixes contain Hot Start Taq DNA Polymerase. This is a Taq DNA polymerase, which has been chemically modified by the addition of heat-labile groups to amino acid residues blocking enzyme activity. The Hot Start Taq DNA Polymerase has ultra low residual activity until thermally activated. Therefore reactions can be assembled at room temperature with no risk for the extension of non-specifically annealed primers or primer dimers and providing higher specificity for DNA amplification. The enzyme is activated by 10 minutes incubation at 95 degrees C.

Can I prepare a qPCR plate in advance and store it for later use when using Luminaris qPCR Master Mixes?

When using a hot start polymerase it is not critical to start reactions immediately after setup. The Hot Start Taq DNA Polymerase in Luminaris qPCR Master Mixes has ultra low residual activity until thermally activated. Therefore reactions can be assembled and stored at room temperature for up to 72 hours with no risk for the extension of non-specifically annealed primers or primer dimers.