Histogene™ Refill Kit, includes dehydration chemicals & stain only

This kit contains only the dehydration chemicals and stain used with the ARCTURUS™ HistoGene™ LCM Frozen Section Staining Kit. Designed as a fast-penetrating stain, this kit provides excellent contrast while preserving nucleic acid integrity and retaining low-abundance mRNAs.

Key product features:
• Optimized process—provide superior staining while preserving RNA
• Comprehensive kit—simplify tissue staining and dehydration
• High-quality RNA yield—retain low-abundance mRNA and maintain RNA integrity
• Versatile application—obtain high-quality RNA from many tissue types
• Modular design—use with other ARCTURUS™ products to produce superior microarray data

Provide Superior Staining while Preserving Nucleic Acids
HistoGene™ Staining Solution has been developed by ARCTURUS™ to stain tissues for LCM subsequently used as sources of nucleic acids. It is a fast-penetrating stain that provides good contrast by differential staining of nuclei (purple) and cytoplasm (light pink). By minimizing the exposure of tissues to water where nucleases may be activated, HistoGene™ stain helps to preserve nucleic integrity that may be otherwise compromised when using longer staining protocols.

Retain Low-abundance mRNA
RT-PCR analysis of specific genes from cells captured from tissues processed using the HistoGene™ LCM Frozen Section Staining Kit shows retention of both low-abundance mRNA and higher-abundance species. The mRNA profile of HistoGene™ samples appears free of degradation.

Maintain RNA Integrity
Tissues prepared for LCM using HistoGene™ LCM Frozen Section Staining Kit reagents, supplies and instructions yield high quality RNA.

Obtain High-quality RNA from Many Tissue Types
ARCTURUS™ has validated the HistoGene™ Kit by examining RNA integrity on many tissue types. All tissues tested to date using the HistoGene™ Kit have yielded quality RNA. Validated tissue types include (Table 1):

Part of the Arcturus™ System for Microgenomics
The HistoGene™ LCM Frozen Section Staining Kit is part of the ARCTURUS™ Complete System of Microgenomics and is designed to seamlessly work together to produce high quality expression microarrays. With the PicoPure™ RNA Isolation Kit, you can recover a high yield of RNA from as few as 10 cells in a minimal volume. The RiboAmp™ RNA Amplification Kit can amplify picograms of your RNA sample into micrograms of amplified RNA (aRNA) that is ready for labeling and hybridization to microarrays. ARCTURUS™ instruments and kits increase the reliability and reproducibility of gene expression studies.

For Research Use Only. Not for use in diagnostics procedures.