LC3B Antibody Kit for Autophagy
LC3B Antibody Kit for Autophagy
Citas y referencias (19)
Invitrogen™

LC3B Antibody Kit for Autophagy

El kit de anticuerpos LC3 para la autofagia incluye un anticuerpo policlonal de conejo contra LC3B que se ha validadoMás información
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Número de catálogoCantidad
L103821 kit
Número de catálogo L10382
Precio (CLP)
611.672
Each
Añadir al carro de la compra
Cantidad:
1 kit
Precio (CLP)
611.672
Each
Añadir al carro de la compra
El kit de anticuerpos LC3 para la autofagia incluye un anticuerpo policlonal de conejo contra LC3B que se ha validado para su uso en microscopía de fluorescencia y análisis e imágenes de alto contenido. También se incluye el difosfato de cloroquina: después del tratamiento con este compuesto, el flujo autofágico normal se interrumpe y los autofagosomas se acumulan como resultado de la interrupción en el flujo autofágico normal.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteSin marcar
FormatoPlaca de 96 pocillos, portaobjetos
Cantidad1 kit
Condiciones de envíoHielo seco
Para utilizar con (equipo)Microscopio confocal, Microscopio de fluorescencia, Instrumentos de alto contenido
Tipo de productoTinte
Unit SizeEach
Contenido y almacenamiento
El kit incluye un anticuerpo policlonal de conejo contra LC3B y difosfato de cloroquina.
  • Almacenar a ≤ -20 °C

    • Preguntas frecuentes

    How does the LC3B Antibody Kit for Autophagy work?

    The LC3B protein plays a critical role in autophagy. This protein resides in the cytosol, but following cleavage and lipidation with phosphatidylethanolamine, LC3 associates with the phagophore. This localization can be used as a general marker for autophagic membranes. The LC3B Antibody Kit for Autophagy includes a rabbit polyclonal antibody against LC3B that has been validated for use in fluorescence microscopy and high content imaging and analysis. The kit also includes chloroquine diphosphate for artificially generating autophagosomes. Following chloroquine diphosphate treatment, lysosomal pH increases and the normal autophagic flux is disrupted, resulting in autophagosome accumulation.

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    Citations & References (19)

    Citations & References
    Abstract
    WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition.
    Authors:Ho H, Kapadia R, Al-Tahan S, Ahmad S, Ganesan AK,
    Journal:J Biol Chem
    PubMed ID:21317285
    'Recent studies implicate a role for WD repeat domain, phosphoinositide-interacting 1 (WIPI1) in the biogenesis of melanosomes, cell type-specific lysosome-related organelles. In this study, we determined that WIPI1, an ATG18 homologue that is shown to localize to both autophagosomes and early endosomes, inhibited mammalian target of rapamycin (MTOR) signaling, leading ... More
    Membrane fusion-mediated autophagy induction enhances morbillivirus cell-to-cell spread.
    Authors:Delpeut S, Rudd PA, Labonté P, von Messling V,
    Journal:J Virol
    PubMed ID:22647692
    In the context of viral infections, autophagy induction can be beneficial or inhibitory. Within the Paramyxoviridae family, only morbilliviruses have been investigated and are reported to induce autophagy. Here we show that morbilliviruses rapidly induce autophagy and require this induction for efficient cell-to-cell spread. Coexpression of both glycoproteins in cells ... More
    Hormonally active vitamin D3 (1alpha,25-dihydroxycholecalciferol) triggers autophagy in human macrophages that inhibits HIV-1 infection.
    Authors:Campbell GR, Spector SA,
    Journal:J Biol Chem
    PubMed ID:21454634
    Autophagy is a self-digestion pathway essential for maintaining cellular homeostasis and cell survival and for degrading intracellular pathogens. Human immunodeficiency virus-1 (HIV-1) may utilize autophagy for replication as the autophagy-related protein-7 (ATG-7), microtubule-associated protein 1 light chain 3, ATG-12, and ATG-16L2 are required for productive HIV-1 infection; however, the effects ... More
    Galectin-8 Senses Phagosomal Damage and Recruits Selective Autophagy Adapter TAX1BP1 To Control Mycobacterium tuberculosis Infection in Macrophages.
    Authors:
    Journal:MBio
    PubMed ID:34225486
    Malpighian Tubule Cells in Overwintering Cave Crickets Troglophilus cavicola (Kollar, 1833) and T. neglectus Krauss, 1879 (Rhaphidophoridae, Ensifera).
    Authors:Lipovšek S, Novak T, Janžekovic F, Weiland N, Leitinger G
    Journal:PLoS One
    PubMed ID:27379687
    'During winter, cave cricket larvae undergo dormancy in subterranean habitats; this dormancy is termed diapause in second year Troglophilus cavicola larvae because they mature during this time, and termed quiescence in T. neglectus, because they mature after dormancy. Here we used electron microscopy to analyze ultrastructural changes in the epithelial ... More
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