Kit de viabilidad de esperma LIVE/DEAD™
Kit de viabilidad de esperma LIVE/DEAD™
Citas y referencias (69)
Invitrogen™

Kit de viabilidad de esperma LIVE/DEAD™

El kit de viabilidad de esperma LIVE/DEAD proporciona un nuevo ensayo basado en la fluorescencia para el análisis de laMás información
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Número de catálogoCantidad
L70111 kit
Número de catálogo L7011
Precio (CLP)
631.684
Each
Añadir al carro de la compra
Cantidad:
1 kit
Precio (CLP)
631.684
Each
Añadir al carro de la compra
El kit de viabilidad de esperma LIVE/DEAD proporciona un nuevo ensayo basado en la fluorescencia para el análisis de la viabilidad y el potencial de fertilización de los espermatozoides por microscopía de fluorescencia o citometría de flujo. El tinte de ácido nucleico permeable en membrana SYBR® 14 etiqueta el esperma vivo con fluorescente verde y el yoduro de propidio no permeable en membrana etiqueta los ácidos nucléicos de esperma con membrana comprometica con fluorescente rojo.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaCélulas eucariotas, células espermáticas
DescripciónKit de viabilidad de esperma LIVE/DEAD™
Método de detecciónFluorescente
Tipo de coloranteOtras etiquetas o colorantes
FormatoTubos, portaobjetos
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
ColorVerde, rojo
Emission516, 617 nm
Para utilizar con (aplicación)Ensayo de viabilidad
Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo
Línea de productosLIVE/DEAD
Tipo de productoKit de viabilidad de espermatozoides
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

What is the fluorescence emission maxima of SYBR 14 dye and propidium iodide?

When bound to DNA, the fluorescence emission maxima of these dyes are 516 nm and 617 nm, respectively. The spectra are available in Figure 1 of the Product Manual.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (69)

Citations & References
Abstract
Seminal plasma addition attenuates the dilution effect in bovine sperm.
Authors:Garner DL,Thomas CA,Gravance CG,Marshall CE,DeJarnette JM,Allen CH
Journal:Theriogenology
PubMed ID:11467516
Dilution of semen to low cell numbers/dose can result in a bull-dependent reduction in the post-thaw viability of cryopreserved bovine spermatozoa. It is possible that essential seminal plasma components are lacking at the greater dilution rates, thereby contributing to the deleterious effects of semen dilution. Ejaculates of 6 Holstein bulls ... More
Identification of amplified restriction fragment length polymorphism markers linked to genes controlling boar sperm viability following cryopreservation.
Authors:Thurston LM, Siggins K, Mileham AJ, Watson PF, Holt WV
Journal:Biol Reprod
PubMed ID:11870056
This study investigated two hypotheses: 1) that consistent between-boar variation in frozen semen quality exists and is genetically determined, and 2) molecular markers linked to genes controlling semen freezability can be identified using amplified restriction fragment length polymorphism (AFLP) technology. Five ejaculates were collected from each of 129 boars. Semen ... More
Comparison of assessment of fowl sperm viability by eosin-nigrosin and dual fluorescence (SYBR-14/PI).
Authors:Chalah T, Brillard JP
Journal:Theriogenology
PubMed ID:10732141
'The kinetics of fowl sperm viability/mortality following short-term and long-term in vitro storage were studied using 2 different staining methods: eosin/nigrosin (observed under light microscopy) and SYBR-14/PI (dual fluorescence). Based on data obtained at 0, 30 min and at 2, 4 and 24 h (T0, T30, T2, T4, and T24) ... More
Seasonal changes of semen quality and freezability in Franches-Montagnes stallions.
Authors:Janett F, Thun R, Bettschen S, Burger D, Hassig M
Journal:Anim Reprod Sci
PubMed ID:12695055
'The objective of this study was to investigate seasonal changes of semen quality parameters in Franches-Montagnes stallions and to compare the freezability of ejaculates collected in autumn and winter. Experiments were performed using 15 stallions from the National Stud Farm in Avenches (Switzerland). Ejaculates were collected and evaluated every month ... More
Flow cytometric assessment of allopurinol susceptibility in Leishmania infantum promastigote.
Authors:Kamau SW, Hurtado M, Müller-Doblies UU, Grimm F, Nunez R
Journal:Cytometry
PubMed ID:10918286
'BACKGROUND: Leishmaniasis is a major tropical and subtropical parasitic disease. Sodium stibogluconate, N-methyl -D-glucamine antimoniate, amphotericin B, pentamidine, and ketoconazole are drugs used to treat this disease. Some of these drugs cause severe adverse side effects and treatment failures are common. Allopurinol, a purine analog, has been used to treat ... More
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